Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas)

• Published in: Nanotoxicology
• Main Authors: Tallec, K., Paul-Pont, Ika, Boulais, M., Le Goïc, Nelly, Gonzalez-Fernandez, C., Le Grand, Fabienne, Bideau, Antoine, Quéré, C., Cassone, A.-L., Lambert, Christophe, Soudant, Philippe, Huvet, Arnaud
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), ANR-15-CE34-0006,Nanoplastics,Microplastiques, nanoplastiques dans l'environnement marin: caractérisation, impacts et évaluation des risques sanitaires.(2015)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: ACL; oyster; flow-cytometry; toxicity; Nanoplastics; paracentrotus-lividus; pacific oyster; reproductive success; spermatozoa; sperm; oxidative stress; fertilization; embryo-larval development; charged polystyrene nanoparticles; motility; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://hal.science/hal-03015209; https://hal.science/hal-03015209/document; https://hal.science/hal-03015209/file/78210.pdf; https://doi.org/10.1080/17435390.2020.1808104

WOS:000560848100001 Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 mu g mL(-1)) of 50-nm polystyrene beads with amine (50-NH(2)beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH(2)beads at 10 mu g mL(-1)induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (-79%) and in the velocity (-62%) compared to control spermatozoa, with an overall drop in embryogenesis success (-59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 mu g mL(-1)and caused a decrease in the percentage of motile spermatozoa (-66%) and in the velocity (-38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment.