Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles
International audience A nanometric revolution is underway, promising technical innovations in a wide range of applications and leading to a potential boost in environmental discharges. The propensity of nanoparticles (NPs) to be transferred throughout trophic chains and to generate toxicity was mai...
Published in: | Environmental Toxicology and Chemistry |
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Main Authors: | , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2020
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Online Access: | https://hal.science/hal-02975506 https://hal.science/hal-02975506/document https://hal.science/hal-02975506/file/2020_FPerrier_EnvToxicolChem.pdf https://doi.org/10.1002/etc.4858 |
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Institut national des sciences de l'Univers: HAL-INSU |
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English |
topic |
Gold nanoparticles Anguilla anguilla RNAseq diet-borne exposure immunity [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
spellingShingle |
Gold nanoparticles Anguilla anguilla RNAseq diet-borne exposure immunity [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Perrier, Fanny Bertucci, Anthony Pierron, Fabien Feurtet‐mazel, Agnès Simon, Olivier Klopp, Christophe, C. Candaudap, Frédéric Pokrovski, Oleg Etcheverria, Bruno Mornet, Stéphane Baudrimont, Magalie Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
topic_facet |
Gold nanoparticles Anguilla anguilla RNAseq diet-borne exposure immunity [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
description |
International audience A nanometric revolution is underway, promising technical innovations in a wide range of applications and leading to a potential boost in environmental discharges. The propensity of nanoparticles (NPs) to be transferred throughout trophic chains and to generate toxicity was mainly assessed in primary consumers, whereas a lack of knowledge for higher trophic levels persists. The present study focused on a predatory fish, the European eel (Anguilla anguilla) exposed to gold NPs (AuNPs; 10 nm, polyethylene glycol–coated) for 21 d at 3 concentration levels in food: 0 (NP0), 1 (NP1), and 10 (NP10) mg Au kg−1. Transfer was assessed by Au quantification in eel tissues, and transcriptomic responses in the liver and brain were revealed by a high‐throughput RNA‐sequencing approach. Eels fed at NP10 presented an erratic feeding behavior, whereas Au quantification only indicated transfer to intestine and kidney of NP1‐exposed eels. Sequencing of RNA was performed in NP0 and NP1 eels. A total of 258 genes and 156 genes were significantly differentially transcribed in response to AuNP trophic exposure in the liver and brain, respectively. Enrichment analysis highlighted modifications in the immune system–related processes in the liver. In addition, results pointed out a shared response of both organs regarding 13 genes, most of them being involved in immune functions. This finding may shed light on the mode of action and toxicity of AuNPs in fish. |
author2 |
Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'écotoxicologie des radionucléides (IRSN/PRP-ENV/SERIS/LECO) Service de Recherche et d'Expertise sur les Risques environnementaux (IRSN/PRP-ENV/SERIS) Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN) Plateforme Bio-Informatique - Génotoul Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL) Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-IR BioInfOmics Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Géosciences Environnement Toulouse (GET) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France-Centre National de la Recherche Scientifique (CNRS) Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB) Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) The authors thank F. Daniel for her daily help and her technical cooperation; M. Gonzalez, P.‐Y. Gourves, and N. Mesmer‐Dudons‐Dufraisse for eel dissections; and H. Bouillard and C. Portier for the experimental room setup. The present study was supported by the CITTOXIC‐Nano program of the French National Research Agency (ANR‐14‐CE21‐0001‐01) and the Investments for the Future Program, within the Cluster of Excellence COTE (ANR‐10‐LABX‐45). F. Perrier was supported by a grant from the French Ministry of Research. ANR-14-CE21-0001,CITTOXIC-Nano,Approches à différentes échelles pour caractériser les interactions cellulaires, le transfert trophique et les impacts toxiques de nanoparticules métalliques chez les organismes aquatiques(2014) ANR-10-LABX-0045,COTE,COntinental To coastal Ecosystems: evolution, adaptability and governance(2010) |
format |
Article in Journal/Newspaper |
author |
Perrier, Fanny Bertucci, Anthony Pierron, Fabien Feurtet‐mazel, Agnès Simon, Olivier Klopp, Christophe, C. Candaudap, Frédéric Pokrovski, Oleg Etcheverria, Bruno Mornet, Stéphane Baudrimont, Magalie |
author_facet |
Perrier, Fanny Bertucci, Anthony Pierron, Fabien Feurtet‐mazel, Agnès Simon, Olivier Klopp, Christophe, C. Candaudap, Frédéric Pokrovski, Oleg Etcheverria, Bruno Mornet, Stéphane Baudrimont, Magalie |
author_sort |
Perrier, Fanny |
title |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_short |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_full |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_fullStr |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_full_unstemmed |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_sort |
transfer and transcriptomic profiling in liver and brain of european eels ( anguilla anguilla ) after diet‐borne exposure to gold nanoparticles |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.science/hal-02975506 https://hal.science/hal-02975506/document https://hal.science/hal-02975506/file/2020_FPerrier_EnvToxicolChem.pdf https://doi.org/10.1002/etc.4858 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 0730-7268 EISSN: 1552-8618 Environmental Toxicology and Chemistry https://hal.science/hal-02975506 Environmental Toxicology and Chemistry, 2020, 39 (12), pp.2450-2461. ⟨10.1002/etc.4858⟩ |
op_relation |
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op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1002/etc.4858 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
39 |
container_issue |
12 |
container_start_page |
2450 |
op_container_end_page |
2461 |
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1797578160695410688 |
spelling |
ftinsu:oai:HAL:hal-02975506v1 2024-04-28T07:55:01+00:00 Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles Perrier, Fanny Bertucci, Anthony Pierron, Fabien Feurtet‐mazel, Agnès Simon, Olivier Klopp, Christophe, C. Candaudap, Frédéric Pokrovski, Oleg Etcheverria, Bruno Mornet, Stéphane Baudrimont, Magalie Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Laboratoire d'écotoxicologie des radionucléides (IRSN/PRP-ENV/SERIS/LECO) Service de Recherche et d'Expertise sur les Risques environnementaux (IRSN/PRP-ENV/SERIS) Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN) Plateforme Bio-Informatique - Génotoul Génopole Toulouse Midi-Pyrénées Auzeville (GENOTOUL) Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT) Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-IR BioInfOmics Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Géosciences Environnement Toulouse (GET) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales Toulouse (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France-Centre National de la Recherche Scientifique (CNRS) Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB) Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) The authors thank F. Daniel for her daily help and her technical cooperation; M. Gonzalez, P.‐Y. Gourves, and N. Mesmer‐Dudons‐Dufraisse for eel dissections; and H. Bouillard and C. Portier for the experimental room setup. The present study was supported by the CITTOXIC‐Nano program of the French National Research Agency (ANR‐14‐CE21‐0001‐01) and the Investments for the Future Program, within the Cluster of Excellence COTE (ANR‐10‐LABX‐45). F. Perrier was supported by a grant from the French Ministry of Research. ANR-14-CE21-0001,CITTOXIC-Nano,Approches à différentes échelles pour caractériser les interactions cellulaires, le transfert trophique et les impacts toxiques de nanoparticules métalliques chez les organismes aquatiques(2014) ANR-10-LABX-0045,COTE,COntinental To coastal Ecosystems: evolution, adaptability and governance(2010) 2020 https://hal.science/hal-02975506 https://hal.science/hal-02975506/document https://hal.science/hal-02975506/file/2020_FPerrier_EnvToxicolChem.pdf https://doi.org/10.1002/etc.4858 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.4858 info:eu-repo/semantics/altIdentifier/pmid/32833228 hal-02975506 https://hal.science/hal-02975506 https://hal.science/hal-02975506/document https://hal.science/hal-02975506/file/2020_FPerrier_EnvToxicolChem.pdf doi:10.1002/etc.4858 PUBMED: 32833228 WOS: 000577675500001 info:eu-repo/semantics/OpenAccess ISSN: 0730-7268 EISSN: 1552-8618 Environmental Toxicology and Chemistry https://hal.science/hal-02975506 Environmental Toxicology and Chemistry, 2020, 39 (12), pp.2450-2461. ⟨10.1002/etc.4858⟩ Gold nanoparticles Anguilla anguilla RNAseq diet-borne exposure immunity [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology info:eu-repo/semantics/article Journal articles 2020 ftinsu https://doi.org/10.1002/etc.4858 2024-04-05T00:40:57Z International audience A nanometric revolution is underway, promising technical innovations in a wide range of applications and leading to a potential boost in environmental discharges. The propensity of nanoparticles (NPs) to be transferred throughout trophic chains and to generate toxicity was mainly assessed in primary consumers, whereas a lack of knowledge for higher trophic levels persists. The present study focused on a predatory fish, the European eel (Anguilla anguilla) exposed to gold NPs (AuNPs; 10 nm, polyethylene glycol–coated) for 21 d at 3 concentration levels in food: 0 (NP0), 1 (NP1), and 10 (NP10) mg Au kg−1. Transfer was assessed by Au quantification in eel tissues, and transcriptomic responses in the liver and brain were revealed by a high‐throughput RNA‐sequencing approach. Eels fed at NP10 presented an erratic feeding behavior, whereas Au quantification only indicated transfer to intestine and kidney of NP1‐exposed eels. Sequencing of RNA was performed in NP0 and NP1 eels. A total of 258 genes and 156 genes were significantly differentially transcribed in response to AuNP trophic exposure in the liver and brain, respectively. Enrichment analysis highlighted modifications in the immune system–related processes in the liver. In addition, results pointed out a shared response of both organs regarding 13 genes, most of them being involved in immune functions. This finding may shed light on the mode of action and toxicity of AuNPs in fish. Article in Journal/Newspaper Anguilla anguilla Institut national des sciences de l'Univers: HAL-INSU Environmental Toxicology and Chemistry 39 12 2450 2461 |