Polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels.
International audience This study investigated the contribution of two biomarkers, bile polycyclic aromatic hydrocarbon (PAH) metabolites and 7-ethoxyresorufin O-deethylase (EROD), activity in the assessment of PAH contaminated sites. European eels (Anguilla anguilla) were caged in a freshwater stre...
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ftinsu:oai:HAL:hal-02879918v1 2023-12-17T10:18:35+01:00 Polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels. Fenet, Hélène Gomez, Elena Rosain, D. Casellas, Claude Hydrosciences Montpellier (HSM) Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) 2006-08-01 https://hal.umontpellier.fr/hal-02879918 https://doi.org/10.1007/s00244-005-0064-1 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00244-005-0064-1 info:eu-repo/semantics/altIdentifier/pmid/16783620 hal-02879918 https://hal.umontpellier.fr/hal-02879918 doi:10.1007/s00244-005-0064-1 PUBMED: 16783620 ISSN: 0090-4341 EISSN: 1432-0703 Archives of Environmental Contamination and Toxicology https://hal.umontpellier.fr/hal-02879918 Archives of Environmental Contamination and Toxicology, 2006, 51 (2), pp.232-236. ⟨10.1007/s00244-005-0064-1⟩ Polycyclic aromatic hydrocarbons Carbon Metabolites [SDV]Life Sciences [q-bio] [SDV.TOX]Life Sciences [q-bio]/Toxicology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology info:eu-repo/semantics/article Journal articles 2006 ftinsu https://doi.org/10.1007/s00244-005-0064-1 2023-11-22T17:40:38Z International audience This study investigated the contribution of two biomarkers, bile polycyclic aromatic hydrocarbon (PAH) metabolites and 7-ethoxyresorufin O-deethylase (EROD), activity in the assessment of PAH contaminated sites. European eels (Anguilla anguilla) were caged in a freshwater stream upstream and downstream from local industrial effluent outlets. Bile PAH metabolites were recorded as fluorescent aromatic compounds by synchronous fluorescence spectroscopy and as a marker for total PAH metabolism: 1-hydroxypyrene (1-OH Pyr) was isolated by high-pressure liquid chromatography and quantified. After 14 and 28 days of caging, EROD activity, bile fluorescence (synchronous fluorometric measurement), and 1-OH Pyr concentrations in bile were higher at the downstream site than at the upstream site. This increase was similar after 2 and 4 weeks of caging. During a reversibility study, EROD activity, bile fluorescence, and 1-OH Pyr concentrations decreased, and this trend was similar for the three markers. These results suggest that PAHs could be the main factor responsible for EROD induction in eels caged at the downstream site. Article in Journal/Newspaper Anguilla anguilla Institut national des sciences de l'Univers: HAL-INSU Archives of Environmental Contamination and Toxicology 51 2 232 236 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Polycyclic aromatic hydrocarbons Carbon Metabolites [SDV]Life Sciences [q-bio] [SDV.TOX]Life Sciences [q-bio]/Toxicology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
spellingShingle |
Polycyclic aromatic hydrocarbons Carbon Metabolites [SDV]Life Sciences [q-bio] [SDV.TOX]Life Sciences [q-bio]/Toxicology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Fenet, Hélène Gomez, Elena Rosain, D. Casellas, Claude Polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels. |
topic_facet |
Polycyclic aromatic hydrocarbons Carbon Metabolites [SDV]Life Sciences [q-bio] [SDV.TOX]Life Sciences [q-bio]/Toxicology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
description |
International audience This study investigated the contribution of two biomarkers, bile polycyclic aromatic hydrocarbon (PAH) metabolites and 7-ethoxyresorufin O-deethylase (EROD), activity in the assessment of PAH contaminated sites. European eels (Anguilla anguilla) were caged in a freshwater stream upstream and downstream from local industrial effluent outlets. Bile PAH metabolites were recorded as fluorescent aromatic compounds by synchronous fluorescence spectroscopy and as a marker for total PAH metabolism: 1-hydroxypyrene (1-OH Pyr) was isolated by high-pressure liquid chromatography and quantified. After 14 and 28 days of caging, EROD activity, bile fluorescence (synchronous fluorometric measurement), and 1-OH Pyr concentrations in bile were higher at the downstream site than at the upstream site. This increase was similar after 2 and 4 weeks of caging. During a reversibility study, EROD activity, bile fluorescence, and 1-OH Pyr concentrations decreased, and this trend was similar for the three markers. These results suggest that PAHs could be the main factor responsible for EROD induction in eels caged at the downstream site. |
author2 |
Hydrosciences Montpellier (HSM) Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Fenet, Hélène Gomez, Elena Rosain, D. Casellas, Claude |
author_facet |
Fenet, Hélène Gomez, Elena Rosain, D. Casellas, Claude |
author_sort |
Fenet, Hélène |
title |
Polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels. |
title_short |
Polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels. |
title_full |
Polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels. |
title_fullStr |
Polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels. |
title_full_unstemmed |
Polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin O-deethylase activity in caged European eels. |
title_sort |
polycyclic aromatic hydrocarbon metabolites and 7-ethoxyresorufin o-deethylase activity in caged european eels. |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.umontpellier.fr/hal-02879918 https://doi.org/10.1007/s00244-005-0064-1 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 0090-4341 EISSN: 1432-0703 Archives of Environmental Contamination and Toxicology https://hal.umontpellier.fr/hal-02879918 Archives of Environmental Contamination and Toxicology, 2006, 51 (2), pp.232-236. ⟨10.1007/s00244-005-0064-1⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00244-005-0064-1 info:eu-repo/semantics/altIdentifier/pmid/16783620 hal-02879918 https://hal.umontpellier.fr/hal-02879918 doi:10.1007/s00244-005-0064-1 PUBMED: 16783620 |
op_doi |
https://doi.org/10.1007/s00244-005-0064-1 |
container_title |
Archives of Environmental Contamination and Toxicology |
container_volume |
51 |
container_issue |
2 |
container_start_page |
232 |
op_container_end_page |
236 |
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1785577683203129344 |