In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement
WOS:000459747700197 International audience Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by th...
Published in: | International Journal of Molecular Sciences |
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Online Access: | https://hal.science/hal-02868576 https://hal.science/hal-02868576/document https://hal.science/hal-02868576/file/Foulon_etal_IJoMS_2019.pdf https://doi.org/10.3390/ijms20010197 |
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ftinsu:oai:HAL:hal-02868576v1 2024-02-11T10:03:03+01:00 In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement Foulon, Valentin Boudry, Pierre Artigaud, Sébastien Guérard, Fabienne Hellio, Claire Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010) 2019 https://hal.science/hal-02868576 https://hal.science/hal-02868576/document https://hal.science/hal-02868576/file/Foulon_etal_IJoMS_2019.pdf https://doi.org/10.3390/ijms20010197 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20010197 hal-02868576 https://hal.science/hal-02868576 https://hal.science/hal-02868576/document https://hal.science/hal-02868576/file/Foulon_etal_IJoMS_2019.pdf doi:10.3390/ijms20010197 info:eu-repo/semantics/OpenAccess ISSN: 1661-6596 EISSN: 1422-0067 International Journal of Molecular Sciences https://hal.science/hal-02868576 International Journal of Molecular Sciences, 2019, 20 (1), pp.197. ⟨10.3390/ijms20010197⟩ ACL Crassostrea gigas Pacific oyster panorama growth transcriptome adhesive binding matrix protein bioadhesive byssus catechol oxidase foot histochemistry pediveliger larvae phragmatopoma-californica proteomic analysis sandcastle worm [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis info:eu-repo/semantics/article Journal articles 2019 ftinsu https://doi.org/10.3390/ijms20010197 2024-01-24T17:33:46Z WOS:000459747700197 International audience Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific International Journal of Molecular Sciences 20 1 197 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
ACL Crassostrea gigas Pacific oyster panorama growth transcriptome adhesive binding matrix protein bioadhesive byssus catechol oxidase foot histochemistry pediveliger larvae phragmatopoma-californica proteomic analysis sandcastle worm [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis |
spellingShingle |
ACL Crassostrea gigas Pacific oyster panorama growth transcriptome adhesive binding matrix protein bioadhesive byssus catechol oxidase foot histochemistry pediveliger larvae phragmatopoma-californica proteomic analysis sandcastle worm [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis Foulon, Valentin Boudry, Pierre Artigaud, Sébastien Guérard, Fabienne Hellio, Claire In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
topic_facet |
ACL Crassostrea gigas Pacific oyster panorama growth transcriptome adhesive binding matrix protein bioadhesive byssus catechol oxidase foot histochemistry pediveliger larvae phragmatopoma-californica proteomic analysis sandcastle worm [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis |
description |
WOS:000459747700197 International audience Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010) |
format |
Article in Journal/Newspaper |
author |
Foulon, Valentin Boudry, Pierre Artigaud, Sébastien Guérard, Fabienne Hellio, Claire |
author_facet |
Foulon, Valentin Boudry, Pierre Artigaud, Sébastien Guérard, Fabienne Hellio, Claire |
author_sort |
Foulon, Valentin |
title |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_short |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_full |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_fullStr |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_full_unstemmed |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_sort |
in silico analysis of pacific oyster (crassostrea gigas) transcriptome over developmental stages reveals candidate genes for larval settlement |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02868576 https://hal.science/hal-02868576/document https://hal.science/hal-02868576/file/Foulon_etal_IJoMS_2019.pdf https://doi.org/10.3390/ijms20010197 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1661-6596 EISSN: 1422-0067 International Journal of Molecular Sciences https://hal.science/hal-02868576 International Journal of Molecular Sciences, 2019, 20 (1), pp.197. ⟨10.3390/ijms20010197⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20010197 hal-02868576 https://hal.science/hal-02868576 https://hal.science/hal-02868576/document https://hal.science/hal-02868576/file/Foulon_etal_IJoMS_2019.pdf doi:10.3390/ijms20010197 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/ijms20010197 |
container_title |
International Journal of Molecular Sciences |
container_volume |
20 |
container_issue |
1 |
container_start_page |
197 |
_version_ |
1790599192374149120 |