Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses
International audience The Antarctic krill, Euphausia superba, is a Southern Ocean endemic species of proven ecological importance to the region. In the context of predicted global warming, it is particularly important to understand how classic biomarkers of heat stress function in this species. In...
Published in: | Cell Stress and Chaperones |
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ftinsu:oai:HAL:hal-02862448v1 2023-05-15T13:40:33+02:00 Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses Toullec, Jean-Yves Cascella, Kévin Ruault, Stéphanie Geffroy, Alexandre Lorieux, David Montagne, Nicolas Ollivaux, Céline Lee, Chi-Ying Adaptation et diversité en milieu marin (ADMM) Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) National Changhua University of Education 2020 https://hal.sorbonne-universite.fr/hal-02862448 https://hal.sorbonne-universite.fr/hal-02862448/document https://hal.sorbonne-universite.fr/hal-02862448/file/Toullec%20et%20al.%20final_2.pdf https://doi.org/10.1007/s12192-020-01103-2 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s12192-020-01103-2 hal-02862448 https://hal.sorbonne-universite.fr/hal-02862448 https://hal.sorbonne-universite.fr/hal-02862448/document https://hal.sorbonne-universite.fr/hal-02862448/file/Toullec%20et%20al.%20final_2.pdf doi:10.1007/s12192-020-01103-2 info:eu-repo/semantics/OpenAccess ISSN: 1355-8145 EISSN: 1466-1268 Cell Stress and Chaperones https://hal.sorbonne-universite.fr/hal-02862448 Cell Stress and Chaperones, 2020, 25 (3), pp.519-531. ⟨10.1007/s12192-020-01103-2⟩ Krill CTmax Hsp70 expression 19 Euphausia superba heat shock [SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/Bioclimatology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2020 ftinsu https://doi.org/10.1007/s12192-020-01103-2 2022-12-14T17:21:49Z International audience The Antarctic krill, Euphausia superba, is a Southern Ocean endemic species of proven ecological importance to the region. In the context of predicted global warming, it is particularly important to understand how classic biomarkers of heat stress function in this species. In this respect, Hsp70s are acknowledged as good candidates. However, previous studies of expression kinetics have not been able to demonstrate significant upregulation of these genes in response to heat shocks at 3 °C and 6 °C for 3 and 6 h. The current work complements these previous results and broadens the prospects for the use of Hsp70s as a relevant marker of thermal shock in this krill species. New experiments demonstrate that induction of Hsp70 isoforms was not detected during exposure to heat shock, but increased expression was observed after several hours of recovery. To complete the analysis of the expression kinetics of the different isoforms, experiments were carried out over short time scales (1 and 2 h at 3 °C and 6 °C) as well as at higher temperatures (9 °C, 12 °C, and 15 °C for 3 h), without any significant response. A 6-week monitoring of animals at 3 °C showed that the time factor is decisive in the establishment of the response. CTmax experiments with incremental times of 1 °C per day or 1 °C every 3 days have shown a particularly high resilience of the animals. The demonstration of the abundance of Hsp70s present before thermal stress in various species of krill, as well as in specimens of E. superba of various origins, showed that the delay in the response in expression could be related to the high constitutive levels of Hsp70 available before the stress experiments. The alternative labelling of the two main isoforms of Hsp70 according to the origin of the animals allowed hypotheses to be put forward on the functioning of thermoregulation in Antarctic krill as well as ice krill. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean Institut national des sciences de l'Univers: HAL-INSU Antarctic Southern Ocean The Antarctic Cell Stress and Chaperones 25 3 519 531 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Krill CTmax Hsp70 expression 19 Euphausia superba heat shock [SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/Bioclimatology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
Krill CTmax Hsp70 expression 19 Euphausia superba heat shock [SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/Bioclimatology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Toullec, Jean-Yves Cascella, Kévin Ruault, Stéphanie Geffroy, Alexandre Lorieux, David Montagne, Nicolas Ollivaux, Céline Lee, Chi-Ying Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
topic_facet |
Krill CTmax Hsp70 expression 19 Euphausia superba heat shock [SDV.EE.BIO]Life Sciences [q-bio]/Ecology environment/Bioclimatology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience The Antarctic krill, Euphausia superba, is a Southern Ocean endemic species of proven ecological importance to the region. In the context of predicted global warming, it is particularly important to understand how classic biomarkers of heat stress function in this species. In this respect, Hsp70s are acknowledged as good candidates. However, previous studies of expression kinetics have not been able to demonstrate significant upregulation of these genes in response to heat shocks at 3 °C and 6 °C for 3 and 6 h. The current work complements these previous results and broadens the prospects for the use of Hsp70s as a relevant marker of thermal shock in this krill species. New experiments demonstrate that induction of Hsp70 isoforms was not detected during exposure to heat shock, but increased expression was observed after several hours of recovery. To complete the analysis of the expression kinetics of the different isoforms, experiments were carried out over short time scales (1 and 2 h at 3 °C and 6 °C) as well as at higher temperatures (9 °C, 12 °C, and 15 °C for 3 h), without any significant response. A 6-week monitoring of animals at 3 °C showed that the time factor is decisive in the establishment of the response. CTmax experiments with incremental times of 1 °C per day or 1 °C every 3 days have shown a particularly high resilience of the animals. The demonstration of the abundance of Hsp70s present before thermal stress in various species of krill, as well as in specimens of E. superba of various origins, showed that the delay in the response in expression could be related to the high constitutive levels of Hsp70 available before the stress experiments. The alternative labelling of the two main isoforms of Hsp70 according to the origin of the animals allowed hypotheses to be put forward on the functioning of thermoregulation in Antarctic krill as well as ice krill. |
author2 |
Adaptation et diversité en milieu marin (ADMM) Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) National Changhua University of Education |
format |
Article in Journal/Newspaper |
author |
Toullec, Jean-Yves Cascella, Kévin Ruault, Stéphanie Geffroy, Alexandre Lorieux, David Montagne, Nicolas Ollivaux, Céline Lee, Chi-Ying |
author_facet |
Toullec, Jean-Yves Cascella, Kévin Ruault, Stéphanie Geffroy, Alexandre Lorieux, David Montagne, Nicolas Ollivaux, Céline Lee, Chi-Ying |
author_sort |
Toullec, Jean-Yves |
title |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_short |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_full |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_fullStr |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_full_unstemmed |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_sort |
antarctic krill (euphausia superba) in a warming ocean: thermotolerance and deciphering hsp70 responses |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.sorbonne-universite.fr/hal-02862448 https://hal.sorbonne-universite.fr/hal-02862448/document https://hal.sorbonne-universite.fr/hal-02862448/file/Toullec%20et%20al.%20final_2.pdf https://doi.org/10.1007/s12192-020-01103-2 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
op_source |
ISSN: 1355-8145 EISSN: 1466-1268 Cell Stress and Chaperones https://hal.sorbonne-universite.fr/hal-02862448 Cell Stress and Chaperones, 2020, 25 (3), pp.519-531. ⟨10.1007/s12192-020-01103-2⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12192-020-01103-2 hal-02862448 https://hal.sorbonne-universite.fr/hal-02862448 https://hal.sorbonne-universite.fr/hal-02862448/document https://hal.sorbonne-universite.fr/hal-02862448/file/Toullec%20et%20al.%20final_2.pdf doi:10.1007/s12192-020-01103-2 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s12192-020-01103-2 |
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Cell Stress and Chaperones |
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