Sperm handling in aquatic animals for artificial reproduction

• Published in: Theriogenology
• Main Authors: Beirao, Jose, Boulais, Myrina, Gallego, Victor, O'Brien, Justine K., Peixoto, Silvio, Robeck, Todd R., Cabrita, Elsa
• Other Authors: Nord University Bodø, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Universitat Politècnica de València = Universitad Politecnica de Valencia = Polytechnic University of Valencia (UPV), Universidade Federal de Pernambuco Recife (UFPE), Centre of Marine Sciences Faro (CCMAR), University of Algarve Portugal, ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2019
• Subjects: Sperm extraction; short-term storage; ACL; cod gadus-morhua; fertilization success; Assisted reproduction; black tiger shrimp; egg ratio; In vitro reproduction; In vitro fertilization; lip-pearl oyster; Litopenaeus stylirostris; spermatozoa motility parameters; Spermatozoa:oocyte ratio; trout oncorhynchus-mykiss; turbot scophthalmus-maximus; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Gadus morhua; Scophthalmus maximus; Turbot
• Online Access: https://hal.science/hal-02749006; https://hal.science/hal-02749006/document; https://hal.science/hal-02749006/file/Beirao_etal_Theriogenology_2019.pdf; https://doi.org/10.1016/j.theriogenology.2019.05.004

WOS:000471207900021 International audience Artificial reproduction involves collection and handling of gametes in a way that secures their quality and maximizes the fertilization outcome. In addition to initial sperm quality, numerous steps can affect the final result of fertilization, from the sperm collection process until gamete mixing (or co-incubation) when the spermatozoon enters or fuses with the oocyte. In this review, we summarize the whole process of sperm handling, from collection until fertilization for fish, penaeid shrimp, bivalve mollusks and marine mammals. To obtain sperm from captive animals, techniques vary widely across taxa, and include stripping by abdominal massage or testis surgical removal in fish, spermatophore collection in penaeid shrimps, gonadal scarification or temperature shock in bivalve mollusks, and voluntary collection via positive reinforcement in mammals. In most cases, special care is needed to avoid contamination by mucus, seawater, urine, or feces that can either activate sperm motility and/or decrease its quality. We also review techniques and extender solutions used for refrigerated storage of sperm across the afore-mentioned taxa. Finally, we give an overview of the different protocols for in vivo and in vitro fertilization including activation of sperm motility and methods for gamete co-incubation. The present study provides valuable information regarding breeder management either for animal production or species conservation.