The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect
WOS:000424459500008 International audience Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This prote...
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Online Access: | https://hal.science/hal-02619272 https://hal.science/hal-02619272/document https://hal.science/hal-02619272/file/Delisle_etal_MB_2018.pdf https://doi.org/10.1007/s10126-017-9789-x |
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ftinsu:oai:HAL:hal-02619272v1 2024-02-11T10:03:03+01:00 The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect Delisle, Lizenn Fuhrmann, Marine Quere, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Università degli Studi di Padova = University of Padua (Unipd) 2018 https://hal.science/hal-02619272 https://hal.science/hal-02619272/document https://hal.science/hal-02619272/file/Delisle_etal_MB_2018.pdf https://doi.org/10.1007/s10126-017-9789-x en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-017-9789-x hal-02619272 https://hal.science/hal-02619272 https://hal.science/hal-02619272/document https://hal.science/hal-02619272/file/Delisle_etal_MB_2018.pdf doi:10.1007/s10126-017-9789-x info:eu-repo/semantics/OpenAccess ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://hal.science/hal-02619272 Marine Biotechnology, 2018, 20 (1), pp.87-97. ⟨10.1007/s10126-017-9789-x⟩ ACL Crassostrea gigas shrimp france membrane expression analysis protein mortality spot syndrome virus cancer hypoxia Ostreid herpes virus Voltage-dependent anion channel Warburg effect [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2018 ftinsu https://doi.org/10.1007/s10126-017-9789-x 2024-01-24T17:34:09Z WOS:000424459500008 International audience Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Marine Biotechnology 20 1 87 97 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
ACL Crassostrea gigas shrimp france membrane expression analysis protein mortality spot syndrome virus cancer hypoxia Ostreid herpes virus Voltage-dependent anion channel Warburg effect [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL Crassostrea gigas shrimp france membrane expression analysis protein mortality spot syndrome virus cancer hypoxia Ostreid herpes virus Voltage-dependent anion channel Warburg effect [SDE.BE]Environmental Sciences/Biodiversity and Ecology Delisle, Lizenn Fuhrmann, Marine Quere, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
topic_facet |
ACL Crassostrea gigas shrimp france membrane expression analysis protein mortality spot syndrome virus cancer hypoxia Ostreid herpes virus Voltage-dependent anion channel Warburg effect [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
WOS:000424459500008 International audience Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Università degli Studi di Padova = University of Padua (Unipd) |
format |
Article in Journal/Newspaper |
author |
Delisle, Lizenn Fuhrmann, Marine Quere, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte |
author_facet |
Delisle, Lizenn Fuhrmann, Marine Quere, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte |
author_sort |
Delisle, Lizenn |
title |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_short |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_full |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_fullStr |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_full_unstemmed |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_sort |
voltage-dependent anion channel (vdac) of pacific oysters crassostrea gigas is upaccumulated during infection by the ostreid herpesvirus-1 (oshv-1): an indicator of the warburg effect |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.science/hal-02619272 https://hal.science/hal-02619272/document https://hal.science/hal-02619272/file/Delisle_etal_MB_2018.pdf https://doi.org/10.1007/s10126-017-9789-x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://hal.science/hal-02619272 Marine Biotechnology, 2018, 20 (1), pp.87-97. ⟨10.1007/s10126-017-9789-x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-017-9789-x hal-02619272 https://hal.science/hal-02619272 https://hal.science/hal-02619272/document https://hal.science/hal-02619272/file/Delisle_etal_MB_2018.pdf doi:10.1007/s10126-017-9789-x |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s10126-017-9789-x |
container_title |
Marine Biotechnology |
container_volume |
20 |
container_issue |
1 |
container_start_page |
87 |
op_container_end_page |
97 |
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1790599191987224576 |