pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas)
WOS:000429100500005 International audience Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and inv...
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Online Access: | https://hal.science/hal-02614271 https://hal.science/hal-02614271/document https://hal.science/hal-02614271/file/Boulais_etal_BO_2018.pdf https://doi.org/10.1242/bio.031427 |
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ftinsu:oai:HAL:hal-02614271v1 2024-02-11T10:03:06+01:00 pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) University of South Bohemia ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2018 https://hal.science/hal-02614271 https://hal.science/hal-02614271/document https://hal.science/hal-02614271/file/Boulais_etal_BO_2018.pdf https://doi.org/10.1242/bio.031427 en eng HAL CCSD Royal Society info:eu-repo/semantics/altIdentifier/doi/10.1242/bio.031427 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02614271 https://hal.science/hal-02614271 https://hal.science/hal-02614271/document https://hal.science/hal-02614271/file/Boulais_etal_BO_2018.pdf doi:10.1242/bio.031427 info:eu-repo/semantics/OpenAccess ISSN: 2046-6390 Biology Open https://hal.science/hal-02614271 Biology Open, 2018, 7 (3), pp.UNSP bio031427. ⟨10.1242/bio.031427⟩ ACL marine fish ocean acidification Spermatozoa Motility pecten-maximus respiration pH Salinity metabolism acrosome reaction internal ph intracellular-ph Ions osmolality sea-urchin sperm Seminal fluid [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2018 ftinsu https://doi.org/10.1242/bio.031427 2024-01-24T17:34:11Z WOS:000429100500005 International audience Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82 +/- 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7%(within a range of 0 to 50%), and pH values above 7.5 (within a range of 4.5 to 9.5). Article in Journal/Newspaper Crassostrea gigas Ocean acidification Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Biology Open |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
ACL marine fish ocean acidification Spermatozoa Motility pecten-maximus respiration pH Salinity metabolism acrosome reaction internal ph intracellular-ph Ions osmolality sea-urchin sperm Seminal fluid [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL marine fish ocean acidification Spermatozoa Motility pecten-maximus respiration pH Salinity metabolism acrosome reaction internal ph intracellular-ph Ions osmolality sea-urchin sperm Seminal fluid [SDE.BE]Environmental Sciences/Biodiversity and Ecology Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
topic_facet |
ACL marine fish ocean acidification Spermatozoa Motility pecten-maximus respiration pH Salinity metabolism acrosome reaction internal ph intracellular-ph Ions osmolality sea-urchin sperm Seminal fluid [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
WOS:000429100500005 International audience Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82 +/- 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7%(within a range of 0 to 50%), and pH values above 7.5 (within a range of 4.5 to 9.5). |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) University of South Bohemia ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky |
author_facet |
Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky |
author_sort |
Boulais, Myrina |
title |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_short |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_full |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_fullStr |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_full_unstemmed |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_sort |
ph controls spermatozoa motility in the pacific oyster (crassostrea gigas) |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.science/hal-02614271 https://hal.science/hal-02614271/document https://hal.science/hal-02614271/file/Boulais_etal_BO_2018.pdf https://doi.org/10.1242/bio.031427 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification Pacific oyster |
genre_facet |
Crassostrea gigas Ocean acidification Pacific oyster |
op_source |
ISSN: 2046-6390 Biology Open https://hal.science/hal-02614271 Biology Open, 2018, 7 (3), pp.UNSP bio031427. ⟨10.1242/bio.031427⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1242/bio.031427 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02614271 https://hal.science/hal-02614271 https://hal.science/hal-02614271/document https://hal.science/hal-02614271/file/Boulais_etal_BO_2018.pdf doi:10.1242/bio.031427 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1242/bio.031427 |
container_title |
Biology Open |
_version_ |
1790599265301561344 |