ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas
WOS:000416186800021 International audience ABC transporters activity and expression have been associated with the multixenobiotic resistance phenotype (MXR). The activity of these proteins leads to a reduction in the intracellular concentration of several xenobiotics, thus reducing their toxicity. H...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , |
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2017
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Online Access: | https://hal.science/hal-02612000 https://doi.org/10.1016/j.fsi.2017.09.014 |
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openpolar |
institution |
Open Polar |
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Institut national des sciences de l'Univers: HAL-INSU |
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ftinsu |
language |
English |
topic |
ACL Oyster hemocytes aquatic organisms gene-expression stress ABC transporter embryonic-cells Immune system cells multixenobiotic resistance mxr mytilus-galloprovincialis p-glycoprotein proteins Sea urchin [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL Oyster hemocytes aquatic organisms gene-expression stress ABC transporter embryonic-cells Immune system cells multixenobiotic resistance mxr mytilus-galloprovincialis p-glycoprotein proteins Sea urchin [SDE.BE]Environmental Sciences/Biodiversity and Ecology Marques-Santos, Luis Fernando Hegaret, Helene Lima-Santos, Leonardo Queiroga, Fernando Ramos Silva, Patricia Mirella, Da ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas |
topic_facet |
ACL Oyster hemocytes aquatic organisms gene-expression stress ABC transporter embryonic-cells Immune system cells multixenobiotic resistance mxr mytilus-galloprovincialis p-glycoprotein proteins Sea urchin [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
WOS:000416186800021 International audience ABC transporters activity and expression have been associated with the multixenobiotic resistance phenotype (MXR). The activity of these proteins leads to a reduction in the intracellular concentration of several xenobiotics, thus reducing their toxicity. However, little attention has been given to the expression of ABC transporters in marine invertebrates and few studies have investigated their role in immune system cells of sea urchins and shellfish bivalves. The aim of the present study was to investigate the activity of the ABC transporters ABCB1 and ABCC1 in immune system cells of sea urchins (coelomocytes) and oysters (hemocytes) from different climatic regions (Brazil and France). Sea urchins and oysters were collected at Paraiba coast; Brazil (Echinometra lucunter and Crassostrea gasar) and Rade of Brest; France (Echinus esculentus and Crassostrea gigas). Coelomocytes and hemocytes were stained with the ABC transporter substrate calcein-AM and dye accumulation analyzed under flow cytometry. Reversin 205 (ABCB1 transporter blocker) and MK571 (ABCC1 transporter blocker) were used as pharmacological tools to investigate ABC transporter activity. A different pattern of calcein accumulation was observed in coelomocytes: phagocytes \textgreater colorless spherulocytes \textgreater vibrate cells \textgreater red spherulocytes. The treatment with MK571 increased calcein fluorescence levels in coelomocytes from both species. However, reversin 205 treatment was not able to increase calcein fluorescence in E. esculentus coelomocytes. These data suggest that ABCC1-like transporter activity is present in both sea urchin species, but ABCB1-like transporter activity might only be present in E. lucunter coelomocytes. The activity of ABCC1-like transporter was observed in all cell types from both bivalve species. However, reversin 205 only increased calcein accumulation in hyalinocytes of the oyster C. gasar, suggesting the absence of ABCB1-like transporter activity in all ... |
author2 |
Universidade Federal da Paraiba (UFPB) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Interdisciplinary Graduate School for the Blue planet (2017) ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017) |
format |
Article in Journal/Newspaper |
author |
Marques-Santos, Luis Fernando Hegaret, Helene Lima-Santos, Leonardo Queiroga, Fernando Ramos Silva, Patricia Mirella, Da |
author_facet |
Marques-Santos, Luis Fernando Hegaret, Helene Lima-Santos, Leonardo Queiroga, Fernando Ramos Silva, Patricia Mirella, Da |
author_sort |
Marques-Santos, Luis Fernando |
title |
ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas |
title_short |
ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas |
title_full |
ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas |
title_fullStr |
ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas |
title_full_unstemmed |
ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas |
title_sort |
abcb1 and abcc1-like transporters in immune system cells from sea urchins echinometra lucunter and echinus esculentus and oysters crassostrea gasar and crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.science/hal-02612000 https://doi.org/10.1016/j.fsi.2017.09.014 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.science/hal-02612000 Fish and Shellfish Immunology, 2017, 70, pp.195-203. ⟨10.1016/j.fsi.2017.09.014⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2017.09.014 hal-02612000 https://hal.science/hal-02612000 doi:10.1016/j.fsi.2017.09.014 |
op_doi |
https://doi.org/10.1016/j.fsi.2017.09.014 |
container_title |
Fish & Shellfish Immunology |
container_volume |
70 |
container_start_page |
195 |
op_container_end_page |
203 |
_version_ |
1790599332448174080 |
spelling |
ftinsu:oai:HAL:hal-02612000v1 2024-02-11T10:03:09+01:00 ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas Marques-Santos, Luis Fernando Hegaret, Helene Lima-Santos, Leonardo Queiroga, Fernando Ramos Silva, Patricia Mirella, Da Universidade Federal da Paraiba (UFPB) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Interdisciplinary Graduate School for the Blue planet (2017) ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017) 2017 https://hal.science/hal-02612000 https://doi.org/10.1016/j.fsi.2017.09.014 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2017.09.014 hal-02612000 https://hal.science/hal-02612000 doi:10.1016/j.fsi.2017.09.014 ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.science/hal-02612000 Fish and Shellfish Immunology, 2017, 70, pp.195-203. ⟨10.1016/j.fsi.2017.09.014⟩ ACL Oyster hemocytes aquatic organisms gene-expression stress ABC transporter embryonic-cells Immune system cells multixenobiotic resistance mxr mytilus-galloprovincialis p-glycoprotein proteins Sea urchin [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2017 ftinsu https://doi.org/10.1016/j.fsi.2017.09.014 2024-01-24T17:34:11Z WOS:000416186800021 International audience ABC transporters activity and expression have been associated with the multixenobiotic resistance phenotype (MXR). The activity of these proteins leads to a reduction in the intracellular concentration of several xenobiotics, thus reducing their toxicity. However, little attention has been given to the expression of ABC transporters in marine invertebrates and few studies have investigated their role in immune system cells of sea urchins and shellfish bivalves. The aim of the present study was to investigate the activity of the ABC transporters ABCB1 and ABCC1 in immune system cells of sea urchins (coelomocytes) and oysters (hemocytes) from different climatic regions (Brazil and France). Sea urchins and oysters were collected at Paraiba coast; Brazil (Echinometra lucunter and Crassostrea gasar) and Rade of Brest; France (Echinus esculentus and Crassostrea gigas). Coelomocytes and hemocytes were stained with the ABC transporter substrate calcein-AM and dye accumulation analyzed under flow cytometry. Reversin 205 (ABCB1 transporter blocker) and MK571 (ABCC1 transporter blocker) were used as pharmacological tools to investigate ABC transporter activity. A different pattern of calcein accumulation was observed in coelomocytes: phagocytes \textgreater colorless spherulocytes \textgreater vibrate cells \textgreater red spherulocytes. The treatment with MK571 increased calcein fluorescence levels in coelomocytes from both species. However, reversin 205 treatment was not able to increase calcein fluorescence in E. esculentus coelomocytes. These data suggest that ABCC1-like transporter activity is present in both sea urchin species, but ABCB1-like transporter activity might only be present in E. lucunter coelomocytes. The activity of ABCC1-like transporter was observed in all cell types from both bivalve species. However, reversin 205 only increased calcein accumulation in hyalinocytes of the oyster C. gasar, suggesting the absence of ABCB1-like transporter activity in all ... Article in Journal/Newspaper Crassostrea gigas Institut national des sciences de l'Univers: HAL-INSU Fish & Shellfish Immunology 70 195 203 |