Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea
WOS:000413256800020 International audience Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. Thes...
Published in: | Algal Research |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2017
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Subjects: | |
Online Access: | https://hal.science/hal-02572314 https://hal.science/hal-02572314/document https://hal.science/hal-02572314/file/Da-Costa_etal_AR-BBaB_2017.pdf https://doi.org/10.1016/j.algal.2017.09.003 |
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ftinsu:oai:HAL:hal-02572314v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
ACL fatty acids larval development biomass production fatty-acid-composition Flow cytometry Growth phase Lipid classes lipid-composition microalga isochrysis-galbana Nitrogen limitation nutrient concentrations oyster crassostrea-gigas pacific oyster pecten-maximus l Tisochrysis lutea triacylglycerol accumulation [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL fatty acids larval development biomass production fatty-acid-composition Flow cytometry Growth phase Lipid classes lipid-composition microalga isochrysis-galbana Nitrogen limitation nutrient concentrations oyster crassostrea-gigas pacific oyster pecten-maximus l Tisochrysis lutea triacylglycerol accumulation [SDE.BE]Environmental Sciences/Biodiversity and Ecology Costa, Fiz, Da Le Grand, Fabienne Quere, Claudie Bougaran, Gael Cadoret, Jean Paul Robert, Rene Soudant, Philippe Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea |
topic_facet |
ACL fatty acids larval development biomass production fatty-acid-composition Flow cytometry Growth phase Lipid classes lipid-composition microalga isochrysis-galbana Nitrogen limitation nutrient concentrations oyster crassostrea-gigas pacific oyster pecten-maximus l Tisochrysis lutea triacylglycerol accumulation [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
WOS:000413256800020 International audience Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates were enhanced at stationary phase in both strains, regardless of N supply. The oleaginous strain (T+) accumulated triacylglycerols; whereas, the standard strain T accumulated alkenones as reserve lipids, especially in Nreduced medium and stationary phase. Each T. lutea strain exhibited an intrinsic specific FA profile in neutral lipids (NL) and, to a lesser extent, in polar lipids (PL) independent of N supply and growth phase. T+ contained more saturated and monounsaturated fatty acids (especially 14:0 and 18:1n-9), but less polyunsaturated fatty acids (18:4n-3 and 22:6n-3) than T. Overall, growth phase induced more changes in fatty acid profiles of both T. lutea strains in NL and PL than N supply in culture medium. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Physiologie et biotechnologie des Algues (PBA) Biotechnologies et Ressources Marines (BRM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Costa, Fiz, Da Le Grand, Fabienne Quere, Claudie Bougaran, Gael Cadoret, Jean Paul Robert, Rene Soudant, Philippe |
author_facet |
Costa, Fiz, Da Le Grand, Fabienne Quere, Claudie Bougaran, Gael Cadoret, Jean Paul Robert, Rene Soudant, Philippe |
author_sort |
Costa, Fiz, Da |
title |
Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea |
title_short |
Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea |
title_full |
Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea |
title_fullStr |
Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea |
title_full_unstemmed |
Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea |
title_sort |
effects of growth phase and nitrogen limitation on biochemical composition of two strains of tisochrysis lutea |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.science/hal-02572314 https://hal.science/hal-02572314/document https://hal.science/hal-02572314/file/Da-Costa_etal_AR-BBaB_2017.pdf https://doi.org/10.1016/j.algal.2017.09.003 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 2211-9264 Algal Research - Biomass, Biofuels and Bioproducts https://hal.science/hal-02572314 Algal Research - Biomass, Biofuels and Bioproducts, 2017, 27, pp.177-189. ⟨10.1016/j.algal.2017.09.003⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2017.09.003 hal-02572314 https://hal.science/hal-02572314 https://hal.science/hal-02572314/document https://hal.science/hal-02572314/file/Da-Costa_etal_AR-BBaB_2017.pdf doi:10.1016/j.algal.2017.09.003 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.algal.2017.09.003 |
container_title |
Algal Research |
container_volume |
27 |
container_start_page |
177 |
op_container_end_page |
189 |
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1790599435751784448 |
spelling |
ftinsu:oai:HAL:hal-02572314v1 2024-02-11T10:03:14+01:00 Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea Costa, Fiz, Da Le Grand, Fabienne Quere, Claudie Bougaran, Gael Cadoret, Jean Paul Robert, Rene Soudant, Philippe Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Physiologie et biotechnologie des Algues (PBA) Biotechnologies et Ressources Marines (BRM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2017 https://hal.science/hal-02572314 https://hal.science/hal-02572314/document https://hal.science/hal-02572314/file/Da-Costa_etal_AR-BBaB_2017.pdf https://doi.org/10.1016/j.algal.2017.09.003 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.algal.2017.09.003 hal-02572314 https://hal.science/hal-02572314 https://hal.science/hal-02572314/document https://hal.science/hal-02572314/file/Da-Costa_etal_AR-BBaB_2017.pdf doi:10.1016/j.algal.2017.09.003 info:eu-repo/semantics/OpenAccess ISSN: 2211-9264 Algal Research - Biomass, Biofuels and Bioproducts https://hal.science/hal-02572314 Algal Research - Biomass, Biofuels and Bioproducts, 2017, 27, pp.177-189. ⟨10.1016/j.algal.2017.09.003⟩ ACL fatty acids larval development biomass production fatty-acid-composition Flow cytometry Growth phase Lipid classes lipid-composition microalga isochrysis-galbana Nitrogen limitation nutrient concentrations oyster crassostrea-gigas pacific oyster pecten-maximus l Tisochrysis lutea triacylglycerol accumulation [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2017 ftinsu https://doi.org/10.1016/j.algal.2017.09.003 2024-01-24T17:34:22Z WOS:000413256800020 International audience Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates were enhanced at stationary phase in both strains, regardless of N supply. The oleaginous strain (T+) accumulated triacylglycerols; whereas, the standard strain T accumulated alkenones as reserve lipids, especially in Nreduced medium and stationary phase. Each T. lutea strain exhibited an intrinsic specific FA profile in neutral lipids (NL) and, to a lesser extent, in polar lipids (PL) independent of N supply and growth phase. T+ contained more saturated and monounsaturated fatty acids (especially 14:0 and 18:1n-9), but less polyunsaturated fatty acids (18:4n-3 and 22:6n-3) than T. Overall, growth phase induced more changes in fatty acid profiles of both T. lutea strains in NL and PL than N supply in culture medium. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Algal Research 27 177 189 |