Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aqua-culture species such as fishes. The aims of the present study were to identify c...
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Language: | English |
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Online Access: | https://hal.science/hal-02554380 https://doi.org/10.1016/j.aquaculture.2014.11.025 |
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ftinsu:oai:HAL:hal-02554380v1 2024-02-11T10:03:07+01:00 Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismaël Quéré, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2015 https://hal.science/hal-02554380 https://doi.org/10.1016/j.aquaculture.2014.11.025 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2014.11.025 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02554380 https://hal.science/hal-02554380 doi:10.1016/j.aquaculture.2014.11.025 ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-02554380 Aquaculture, 2015, 437, pp.201-207. ⟨10.1016/j.aquaculture.2014.11.025⟩ ACL Crassostrea gigas Oocyte quality Trochophore Lipid Gene expression ATP [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2015 ftinsu https://doi.org/10.1016/j.aquaculture.2014.11.025 2024-01-24T17:34:30Z Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aqua-culture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total lipids and triglycerides for 85% of the neutral lipids. Oocyte fertilization capacity was assessed by determining the D-larval yield. High inter-individual variation of D-larval yield was observed (ranging from 1% to 58%). D-larval yield was positively correlated with the percentage of motile trochophores (R-2 = 0.237) and with trochophore velocity of the average path (VAP; R-2 = 0.255) and negatively correlated with oocyte total steryl esters (R-2 = 0.244). The use of a multivariate model, including D-larval yield, trochophore VAP and total steryl esters, led to a higher coefficient of determination (R-2 = 0.352), highlighting that the prediction of embryo development in mollusks is a complex process requiring sophisticated multi-factor models. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Aquaculture 437 201 207 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
ACL Crassostrea gigas Oocyte quality Trochophore Lipid Gene expression ATP [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL Crassostrea gigas Oocyte quality Trochophore Lipid Gene expression ATP [SDE.BE]Environmental Sciences/Biodiversity and Ecology Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismaël Quéré, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
topic_facet |
ACL Crassostrea gigas Oocyte quality Trochophore Lipid Gene expression ATP [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aqua-culture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total lipids and triglycerides for 85% of the neutral lipids. Oocyte fertilization capacity was assessed by determining the D-larval yield. High inter-individual variation of D-larval yield was observed (ranging from 1% to 58%). D-larval yield was positively correlated with the percentage of motile trochophores (R-2 = 0.237) and with trochophore velocity of the average path (VAP; R-2 = 0.255) and negatively correlated with oocyte total steryl esters (R-2 = 0.244). The use of a multivariate model, including D-larval yield, trochophore VAP and total steryl esters, led to a higher coefficient of determination (R-2 = 0.352), highlighting that the prediction of embryo development in mollusks is a complex process requiring sophisticated multi-factor models. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismaël Quéré, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc |
author_facet |
Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismaël Quéré, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc |
author_sort |
Boulais, Myrina |
title |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_short |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_full |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_fullStr |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_sort |
assessment of oocyte and trochophore quality in pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-02554380 https://doi.org/10.1016/j.aquaculture.2014.11.025 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-02554380 Aquaculture, 2015, 437, pp.201-207. ⟨10.1016/j.aquaculture.2014.11.025⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2014.11.025 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02554380 https://hal.science/hal-02554380 doi:10.1016/j.aquaculture.2014.11.025 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2014.11.025 |
container_title |
Aquaculture |
container_volume |
437 |
container_start_page |
201 |
op_container_end_page |
207 |
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1790599277682098176 |