Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas
In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we...
Published in: | Biology of Reproduction |
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Language: | English |
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ftinsu:oai:HAL:hal-02554379v1 2024-02-11T10:03:09+01:00 Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas Boulais, Myrina Soudant, Philippe Le Goïc, Nelly Quéré, Claudie Boudry, Pierre Suquet, Marc Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2015 https://hal.science/hal-02554379 https://doi.org/10.1095/biolreprod.115.128538 en eng HAL CCSD Society for the Study of Reproduction info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.115.128538 info:eu-repo/semantics/altIdentifier/pmid/26423125 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02554379 https://hal.science/hal-02554379 doi:10.1095/biolreprod.115.128538 PUBMED: 26423125 ISSN: 0006-3363 EISSN: 1529-7268 Biology of Reproduction https://hal.science/hal-02554379 Biology of Reproduction, 2015, 93 (5), pp.118. ⟨10.1095/biolreprod.115.128538⟩ Crassostrea gigas intracellular ATP content mitochondrial membrane potential oxidative phosphorylation sperm motility ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2015 ftinsu https://doi.org/10.1095/biolreprod.115.128538 2024-01-24T17:34:30Z In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied: i) changes in spermatozoa mitochondrial activity (i.e., mitochondrial membrane potential, MMP) and intracellular ATP content in relation to motion parameters and ii) the involvement of OXPHOS for spermatozoa movement using CCCP. The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a constant level. Conversely, spermatozoa ATP content decreased steadily during the first 9 h post activation and was maintained at this level during the following hours of the movement phase. When OXPHOS was decoupled by CCCP, the movement of spermatozoa was maintained 2 h and totally stopped after 4 h of incubation, whereas spermatozoa were still motile in the control after 4 h. Our results suggest that ATP sustaining flagellar movement of spermatozoa may partially originate from glycolysis or from mobilization of stored ATP or potential phosphagens during the first 2 h of movement as OXPHOS was decoupled by CCCP. However, OXPHOS is required to sustain the long motility phase of Pacific oyster spermatozoa. In addition, spermatozoa may hydrolyse intracellular ATP content during the early part of the movement phase, stimulating mitochondrial activity. This stimulation seems to be involved in sustaining a high ATP level until the end of the motility phase. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Biology of Reproduction 93 5 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Crassostrea gigas intracellular ATP content mitochondrial membrane potential oxidative phosphorylation sperm motility ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Crassostrea gigas intracellular ATP content mitochondrial membrane potential oxidative phosphorylation sperm motility ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology Boulais, Myrina Soudant, Philippe Le Goïc, Nelly Quéré, Claudie Boudry, Pierre Suquet, Marc Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
topic_facet |
Crassostrea gigas intracellular ATP content mitochondrial membrane potential oxidative phosphorylation sperm motility ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied: i) changes in spermatozoa mitochondrial activity (i.e., mitochondrial membrane potential, MMP) and intracellular ATP content in relation to motion parameters and ii) the involvement of OXPHOS for spermatozoa movement using CCCP. The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a constant level. Conversely, spermatozoa ATP content decreased steadily during the first 9 h post activation and was maintained at this level during the following hours of the movement phase. When OXPHOS was decoupled by CCCP, the movement of spermatozoa was maintained 2 h and totally stopped after 4 h of incubation, whereas spermatozoa were still motile in the control after 4 h. Our results suggest that ATP sustaining flagellar movement of spermatozoa may partially originate from glycolysis or from mobilization of stored ATP or potential phosphagens during the first 2 h of movement as OXPHOS was decoupled by CCCP. However, OXPHOS is required to sustain the long motility phase of Pacific oyster spermatozoa. In addition, spermatozoa may hydrolyse intracellular ATP content during the early part of the movement phase, stimulating mitochondrial activity. This stimulation seems to be involved in sustaining a high ATP level until the end of the motility phase. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Soudant, Philippe Le Goïc, Nelly Quéré, Claudie Boudry, Pierre Suquet, Marc |
author_facet |
Boulais, Myrina Soudant, Philippe Le Goïc, Nelly Quéré, Claudie Boudry, Pierre Suquet, Marc |
author_sort |
Boulais, Myrina |
title |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_short |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_full |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_fullStr |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_full_unstemmed |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_sort |
involvement of mitochondrial activity and oxphos in atp synthesis during the motility phase of spermatozoa in the pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-02554379 https://doi.org/10.1095/biolreprod.115.128538 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0006-3363 EISSN: 1529-7268 Biology of Reproduction https://hal.science/hal-02554379 Biology of Reproduction, 2015, 93 (5), pp.118. ⟨10.1095/biolreprod.115.128538⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.115.128538 info:eu-repo/semantics/altIdentifier/pmid/26423125 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02554379 https://hal.science/hal-02554379 doi:10.1095/biolreprod.115.128538 PUBMED: 26423125 |
op_doi |
https://doi.org/10.1095/biolreprod.115.128538 |
container_title |
Biology of Reproduction |
container_volume |
93 |
container_issue |
5 |
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1790599314954780672 |