A microarray-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus
International audience The European clam, Ruditapes decussatus is a species with a high commercial importance in Portugal and other Southern European countries. Its production is almost exclusively based on natural recruitment, which is subject to high annual fluctuations. Increased knowledge of the...
Published in: | PLoS ONE |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2014
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Online Access: | https://hal.science/hal-02549129 https://doi.org/10.1371/journal.pone.0092202 |
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ftinsu:oai:HAL:hal-02549129v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
ACL VASA-LIKE GENE CARPET SHELL CLAM CRASSOSTREA-GIGAS GONADAL DEVELOPMENT REPRODUCTIVE-CYCLE MATURATION OYSTER EXPRESSION GERMLINE MOLLUSK [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL VASA-LIKE GENE CARPET SHELL CLAM CRASSOSTREA-GIGAS GONADAL DEVELOPMENT REPRODUCTIVE-CYCLE MATURATION OYSTER EXPRESSION GERMLINE MOLLUSK [SDE.BE]Environmental Sciences/Biodiversity and Ecology Sousa, Joana Teixeira, De Milan, Massimo Bargelloni, Luca Pauletto, Marianna Matias, Domitília Joaquim, Sandra Matias, Ana Margarete Quillien, Virgile Leitão, Alexandra Huvet, Arnaud A microarray-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus |
topic_facet |
ACL VASA-LIKE GENE CARPET SHELL CLAM CRASSOSTREA-GIGAS GONADAL DEVELOPMENT REPRODUCTIVE-CYCLE MATURATION OYSTER EXPRESSION GERMLINE MOLLUSK [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience The European clam, Ruditapes decussatus is a species with a high commercial importance in Portugal and other Southern European countries. Its production is almost exclusively based on natural recruitment, which is subject to high annual fluctuations. Increased knowledge of the natural reproductive cycle of R. decussatus and its molecular mechanisms would be particularly important in providing new highly valuable genomic information for better understanding the regulation of reproduction in this economically important aquaculture species. In this study, the transcriptomic bases of R. decussatus reproduction have been analysed using a custom oligonucleotide microarray representing 51,678 assembled contigs. Microarray analyses were performed in four gonadal maturation stages from two different Portuguese wild populations, characterized by different responses to spawning induction when used as progenitors in hatchery. A comparison between the two populations elucidated a specific pathway involved in the recognition signals and binding between the oocyte and components of the sperm plasma membrane. We suggest that this pathway can explain part of the differences in terms of spawning induction success between the two populations. In addition, sexes and reproductive stages were compared and a correlation between mRNA levels and gonadal area was investigated. The lists of differentially expressed genes revealed that sex explains most of the variance in gonadal gene expression. Additionally, genes like Foxl2, vitellogenin, condensing 2, mitotic apparatus protein p62, Cep57, sperm associated antigens 6, 16 and 17, motile sperm domain containing protein 2, sperm surface protein Sp17, sperm flagellar proteins 1 and 2 and dpy-30, were identified as being correlated with the gonad area and therefore supposedly with the number and/or the size of the gametes produced. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Instituto Português de Investigação do Mar e da Atmosfera (IPMA) Università degli Studi di Padova = University of Padua (Unipd) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) |
format |
Article in Journal/Newspaper |
author |
Sousa, Joana Teixeira, De Milan, Massimo Bargelloni, Luca Pauletto, Marianna Matias, Domitília Joaquim, Sandra Matias, Ana Margarete Quillien, Virgile Leitão, Alexandra Huvet, Arnaud |
author_facet |
Sousa, Joana Teixeira, De Milan, Massimo Bargelloni, Luca Pauletto, Marianna Matias, Domitília Joaquim, Sandra Matias, Ana Margarete Quillien, Virgile Leitão, Alexandra Huvet, Arnaud |
author_sort |
Sousa, Joana Teixeira, De |
title |
A microarray-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus |
title_short |
A microarray-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus |
title_full |
A microarray-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus |
title_fullStr |
A microarray-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus |
title_full_unstemmed |
A microarray-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus |
title_sort |
microarray-based analysis of gametogenesis in two portuguese populations of the european clam ruditapes decussatus |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.science/hal-02549129 https://doi.org/10.1371/journal.pone.0092202 |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-02549129 PLoS ONE, 2014, 9 (3), pp.e92202. ⟨10.1371/journal.pone.0092202⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0092202 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02549129 https://hal.science/hal-02549129 doi:10.1371/journal.pone.0092202 PUBMEDCENTRAL: PMC3958495 |
op_doi |
https://doi.org/10.1371/journal.pone.0092202 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
3 |
container_start_page |
e92202 |
_version_ |
1790599464742813696 |
spelling |
ftinsu:oai:HAL:hal-02549129v1 2024-02-11T10:03:16+01:00 A microarray-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus Sousa, Joana Teixeira, De Milan, Massimo Bargelloni, Luca Pauletto, Marianna Matias, Domitília Joaquim, Sandra Matias, Ana Margarete Quillien, Virgile Leitão, Alexandra Huvet, Arnaud Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Instituto Português de Investigação do Mar e da Atmosfera (IPMA) Università degli Studi di Padova = University of Padua (Unipd) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2014 https://hal.science/hal-02549129 https://doi.org/10.1371/journal.pone.0092202 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0092202 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-02549129 https://hal.science/hal-02549129 doi:10.1371/journal.pone.0092202 PUBMEDCENTRAL: PMC3958495 ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-02549129 PLoS ONE, 2014, 9 (3), pp.e92202. ⟨10.1371/journal.pone.0092202⟩ ACL VASA-LIKE GENE CARPET SHELL CLAM CRASSOSTREA-GIGAS GONADAL DEVELOPMENT REPRODUCTIVE-CYCLE MATURATION OYSTER EXPRESSION GERMLINE MOLLUSK [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2014 ftinsu https://doi.org/10.1371/journal.pone.0092202 2024-01-24T17:34:32Z International audience The European clam, Ruditapes decussatus is a species with a high commercial importance in Portugal and other Southern European countries. Its production is almost exclusively based on natural recruitment, which is subject to high annual fluctuations. Increased knowledge of the natural reproductive cycle of R. decussatus and its molecular mechanisms would be particularly important in providing new highly valuable genomic information for better understanding the regulation of reproduction in this economically important aquaculture species. In this study, the transcriptomic bases of R. decussatus reproduction have been analysed using a custom oligonucleotide microarray representing 51,678 assembled contigs. Microarray analyses were performed in four gonadal maturation stages from two different Portuguese wild populations, characterized by different responses to spawning induction when used as progenitors in hatchery. A comparison between the two populations elucidated a specific pathway involved in the recognition signals and binding between the oocyte and components of the sperm plasma membrane. We suggest that this pathway can explain part of the differences in terms of spawning induction success between the two populations. In addition, sexes and reproductive stages were compared and a correlation between mRNA levels and gonadal area was investigated. The lists of differentially expressed genes revealed that sex explains most of the variance in gonadal gene expression. Additionally, genes like Foxl2, vitellogenin, condensing 2, mitotic apparatus protein p62, Cep57, sperm associated antigens 6, 16 and 17, motile sperm domain containing protein 2, sperm surface protein Sp17, sperm flagellar proteins 1 and 2 and dpy-30, were identified as being correlated with the gonad area and therefore supposedly with the number and/or the size of the gametes produced. Article in Journal/Newspaper Crassostrea gigas Institut national des sciences de l'Univers: HAL-INSU Vasa ENVELOPE(25.177,25.177,67.587,67.587) PLoS ONE 9 3 e92202 |