Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome
International audience Background As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some...
Published in: | BMC Genomics |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2020
|
Subjects: | |
Online Access: | https://hal.science/hal-02432454 https://hal.science/hal-02432454/document https://hal.science/hal-02432454/file/DeLorgeril-ACCEPTED-BMCGenom-Differential.pdf https://doi.org/10.1186/s12864-020-6471-x |
id |
ftinsu:oai:HAL:hal-02432454v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Pacific oyster Oyster disease Resistance OsHV-1 Antiviral molecular pathways Invertebrate immunity Acl [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Pacific oyster Oyster disease Resistance OsHV-1 Antiviral molecular pathways Invertebrate immunity Acl [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology de Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Stenger, Pierre-Louis Dégremont, Lionel Montagnani, Caroline Escoubas, Jean-Michel Haffner, Philippe Allienne, Jean-François Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jeremie Gueguen, Yannick Mitta, Guillaume Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome |
topic_facet |
Pacific oyster Oyster disease Resistance OsHV-1 Antiviral molecular pathways Invertebrate immunity Acl [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Background As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. Conclusions We found differences in basal expression levels of genes related to TLR-NF kappa B, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function. |
author2 |
Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD) Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) The present study was supported by the ANR project DECIPHER (ANR-14-CE19–0023), by the ANR project DECICOMP and by Ifremer, CNRS, Université de Montpellier and Université de Perpignan via Domitia. ANR-14-CE19-0023,DECIPHER,Déchiffrage des maladies multifactorielles: cas des mortalités de l'huître(2014) |
format |
Article in Journal/Newspaper |
author |
de Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Stenger, Pierre-Louis Dégremont, Lionel Montagnani, Caroline Escoubas, Jean-Michel Haffner, Philippe Allienne, Jean-François Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jeremie Gueguen, Yannick Mitta, Guillaume |
author_facet |
de Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Stenger, Pierre-Louis Dégremont, Lionel Montagnani, Caroline Escoubas, Jean-Michel Haffner, Philippe Allienne, Jean-François Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jeremie Gueguen, Yannick Mitta, Guillaume |
author_sort |
de Lorgeril, Julien |
title |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome |
title_short |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome |
title_full |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome |
title_fullStr |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome |
title_full_unstemmed |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome |
title_sort |
differential basal expression of immune genes confers crassostrea gigas resistance to pacific oyster mortality syndrome |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.science/hal-02432454 https://hal.science/hal-02432454/document https://hal.science/hal-02432454/file/DeLorgeril-ACCEPTED-BMCGenom-Differential.pdf https://doi.org/10.1186/s12864-020-6471-x |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1471-2164 BMC Genomics https://hal.science/hal-02432454 BMC Genomics, 2020, 21 (1), pp.63. ⟨10.1186/s12864-020-6471-x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-020-6471-x hal-02432454 https://hal.science/hal-02432454 https://hal.science/hal-02432454/document https://hal.science/hal-02432454/file/DeLorgeril-ACCEPTED-BMCGenom-Differential.pdf doi:10.1186/s12864-020-6471-x |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s12864-020-6471-x |
container_title |
BMC Genomics |
container_volume |
21 |
container_issue |
1 |
_version_ |
1796941184035192832 |
spelling |
ftinsu:oai:HAL:hal-02432454v1 2024-04-21T08:00:45+00:00 Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome de Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Stenger, Pierre-Louis Dégremont, Lionel Montagnani, Caroline Escoubas, Jean-Michel Haffner, Philippe Allienne, Jean-François Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jeremie Gueguen, Yannick Mitta, Guillaume Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Ecosystèmes Insulaires Océaniens (UMR 241) (EIO) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé Papeete (ILM) Institut de Recherche pour le Développement (IRD) Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) The present study was supported by the ANR project DECIPHER (ANR-14-CE19–0023), by the ANR project DECICOMP and by Ifremer, CNRS, Université de Montpellier and Université de Perpignan via Domitia. ANR-14-CE19-0023,DECIPHER,Déchiffrage des maladies multifactorielles: cas des mortalités de l'huître(2014) 2020-01-20 https://hal.science/hal-02432454 https://hal.science/hal-02432454/document https://hal.science/hal-02432454/file/DeLorgeril-ACCEPTED-BMCGenom-Differential.pdf https://doi.org/10.1186/s12864-020-6471-x en eng HAL CCSD BioMed Central info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-020-6471-x hal-02432454 https://hal.science/hal-02432454 https://hal.science/hal-02432454/document https://hal.science/hal-02432454/file/DeLorgeril-ACCEPTED-BMCGenom-Differential.pdf doi:10.1186/s12864-020-6471-x info:eu-repo/semantics/OpenAccess ISSN: 1471-2164 BMC Genomics https://hal.science/hal-02432454 BMC Genomics, 2020, 21 (1), pp.63. ⟨10.1186/s12864-020-6471-x⟩ Pacific oyster Oyster disease Resistance OsHV-1 Antiviral molecular pathways Invertebrate immunity Acl [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2020 ftinsu https://doi.org/10.1186/s12864-020-6471-x 2024-04-05T00:43:15Z International audience Background As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. Conclusions We found differences in basal expression levels of genes related to TLR-NF kappa B, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU BMC Genomics 21 1 |