Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas
International audience The impact of dispersed crude oil and dispersant on adult Pacific oysters, Crassostrea gigas, was evaluated through an integrative biomarker approach including (1) biochemical (plasma catecholase- and laccase-type phenoloxidase and superoxide dismutase), (2) histological (dige...
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ftinsu:oai:HAL:hal-02335958v1 2023-05-15T15:58:09+02:00 Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas Luna-Acosta, Andrea Bustamante, Paco Thomas-Guyon, Hélène Zaldibar, Beñat Izagirre, Urtzi Marigómez, Ionan LIttoral ENvironnement et Sociétés - UMR 7266 (LIENSs) Institut national des sciences de l'Univers (INSU - CNRS)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU) 2017-11 https://hal.science/hal-02335958 https://hal.science/hal-02335958/document https://hal.science/hal-02335958/file/Luna%20Acosta%20et%20al%202017%20STOTEN.pdf https://doi.org/10.1016/j.scitotenv.2017.04.001 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2017.04.001 hal-02335958 https://hal.science/hal-02335958 https://hal.science/hal-02335958/document https://hal.science/hal-02335958/file/Luna%20Acosta%20et%20al%202017%20STOTEN.pdf doi:10.1016/j.scitotenv.2017.04.001 info:eu-repo/semantics/OpenAccess ISSN: 0048-9697 EISSN: 1879-1026 Science of the Total Environment https://hal.science/hal-02335958 Science of the Total Environment, 2017, 598, pp.713-721. ⟨10.1016/j.scitotenv.2017.04.001⟩ invertebrate biomarker Integrated biomarker response dispersant oil spill bivalve [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDE.MCG]Environmental Sciences/Global Changes info:eu-repo/semantics/article Journal articles 2017 ftinsu https://doi.org/10.1016/j.scitotenv.2017.04.001 2023-02-15T19:30:51Z International audience The impact of dispersed crude oil and dispersant on adult Pacific oysters, Crassostrea gigas, was evaluated through an integrative biomarker approach including (1) biochemical (plasma catecholase- and laccase-type phenoloxidase and superoxide dismutase), (2) histological (digestive cell lysosomal responses, digestive gland histopathology) and (3) physiological (flesh condition index) endpoints in the haemolymph and digestive gland. Adult oysters were exposed to non-contaminated water (control), chemically-dispersed oil (Brut Arabian Light), mechanically-dispersed oil and dispersant (FINASOL®) alone for 2 days, and further depurated in noncontaminated water for 4 weeks. After exposure to chemically and mechanically dispersed oil oysters exhibited induction of plasma laccase-type phenoloxidase and superoxide dismutase activities, enlargement of digestive cell lysosomes, lipofuscin accumulation, reduced neutral lipid content and atrophy of digestive gland diverticula; more markedly on exposure to chemically dispersed oil. From the studied biomarkers, only lysosomal biomarkers were significantly affected after exposure to the dispersant alone. This included lysosomal enlargement, neutral lipid depletion and lipofuscin accumulation in the digestive gland epithelium. A recovery of plasma enzyme activities was observed after 4 weeks of depuration. The integrative biological response index indicated that chemically dispersed oil caused significantly higher stress to C. gigas than the mechanically-dispersed one or the dispersant alone; nevertheless, the response seems to be reversible after depuration. Article in Journal/Newspaper Crassostrea gigas Institut national des sciences de l'Univers: HAL-INSU Pacific Science of The Total Environment 598 713 721 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
invertebrate biomarker Integrated biomarker response dispersant oil spill bivalve [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDE.MCG]Environmental Sciences/Global Changes |
spellingShingle |
invertebrate biomarker Integrated biomarker response dispersant oil spill bivalve [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDE.MCG]Environmental Sciences/Global Changes Luna-Acosta, Andrea Bustamante, Paco Thomas-Guyon, Hélène Zaldibar, Beñat Izagirre, Urtzi Marigómez, Ionan Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas |
topic_facet |
invertebrate biomarker Integrated biomarker response dispersant oil spill bivalve [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDE.MCG]Environmental Sciences/Global Changes |
description |
International audience The impact of dispersed crude oil and dispersant on adult Pacific oysters, Crassostrea gigas, was evaluated through an integrative biomarker approach including (1) biochemical (plasma catecholase- and laccase-type phenoloxidase and superoxide dismutase), (2) histological (digestive cell lysosomal responses, digestive gland histopathology) and (3) physiological (flesh condition index) endpoints in the haemolymph and digestive gland. Adult oysters were exposed to non-contaminated water (control), chemically-dispersed oil (Brut Arabian Light), mechanically-dispersed oil and dispersant (FINASOL®) alone for 2 days, and further depurated in noncontaminated water for 4 weeks. After exposure to chemically and mechanically dispersed oil oysters exhibited induction of plasma laccase-type phenoloxidase and superoxide dismutase activities, enlargement of digestive cell lysosomes, lipofuscin accumulation, reduced neutral lipid content and atrophy of digestive gland diverticula; more markedly on exposure to chemically dispersed oil. From the studied biomarkers, only lysosomal biomarkers were significantly affected after exposure to the dispersant alone. This included lysosomal enlargement, neutral lipid depletion and lipofuscin accumulation in the digestive gland epithelium. A recovery of plasma enzyme activities was observed after 4 weeks of depuration. The integrative biological response index indicated that chemically dispersed oil caused significantly higher stress to C. gigas than the mechanically-dispersed one or the dispersant alone; nevertheless, the response seems to be reversible after depuration. |
author2 |
LIttoral ENvironnement et Sociétés - UMR 7266 (LIENSs) Institut national des sciences de l'Univers (INSU - CNRS)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU) |
format |
Article in Journal/Newspaper |
author |
Luna-Acosta, Andrea Bustamante, Paco Thomas-Guyon, Hélène Zaldibar, Beñat Izagirre, Urtzi Marigómez, Ionan |
author_facet |
Luna-Acosta, Andrea Bustamante, Paco Thomas-Guyon, Hélène Zaldibar, Beñat Izagirre, Urtzi Marigómez, Ionan |
author_sort |
Luna-Acosta, Andrea |
title |
Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas |
title_short |
Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas |
title_full |
Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas |
title_fullStr |
Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas |
title_full_unstemmed |
Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas |
title_sort |
integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in pacific oysters, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.science/hal-02335958 https://hal.science/hal-02335958/document https://hal.science/hal-02335958/file/Luna%20Acosta%20et%20al%202017%20STOTEN.pdf https://doi.org/10.1016/j.scitotenv.2017.04.001 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0048-9697 EISSN: 1879-1026 Science of the Total Environment https://hal.science/hal-02335958 Science of the Total Environment, 2017, 598, pp.713-721. ⟨10.1016/j.scitotenv.2017.04.001⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2017.04.001 hal-02335958 https://hal.science/hal-02335958 https://hal.science/hal-02335958/document https://hal.science/hal-02335958/file/Luna%20Acosta%20et%20al%202017%20STOTEN.pdf doi:10.1016/j.scitotenv.2017.04.001 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.scitotenv.2017.04.001 |
container_title |
Science of The Total Environment |
container_volume |
598 |
container_start_page |
713 |
op_container_end_page |
721 |
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1766393890441527296 |