High-Resolution Melting of 18S rDNA sequences (18S-HRM) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey
International audience It is particularly difficult to identify bivalve species at early development stages (larvae and spat) by morphological examination. Targeting genetic marker commonly used for species identification, we developed a cheap and user friendly tool for bivalve identification throug...
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Online Access: | https://univ-perp.hal.science/hal-01740083 https://doi.org/10.1007/s00227-017-3162-5 |
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ftinsu:oai:HAL:hal-01740083v1 2024-02-11T10:03:14+01:00 High-Resolution Melting of 18S rDNA sequences (18S-HRM) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey Meistertzheim, Anne-Leila Héritier, Laurent Lejart, Morgane Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centre de recherches insulaires et observatoire de l'environnement (CRIOBE) Université de Perpignan Via Domitia (UPVD)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Centre de Formation et de Recherche sur les Environnements Méditérranéens (CEFREM) Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) France Energies Marines Brest 2017-06 https://univ-perp.hal.science/hal-01740083 https://doi.org/10.1007/s00227-017-3162-5 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00227-017-3162-5 hal-01740083 https://univ-perp.hal.science/hal-01740083 doi:10.1007/s00227-017-3162-5 ISSN: 0025-3162 EISSN: 1432-1793 Marine Biology https://univ-perp.hal.science/hal-01740083 Marine Biology, 2017, 164 (6), pp.133. ⟨10.1007/s00227-017-3162-5⟩ ACL Bivalve Oyster Reef Bivalve Species Oyster Species Subtidal Area [SDV]Life Sciences [q-bio] [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2017 ftinsu https://doi.org/10.1007/s00227-017-3162-5 2024-01-24T17:37:30Z International audience It is particularly difficult to identify bivalve species at early development stages (larvae and spat) by morphological examination. Targeting genetic marker commonly used for species identification, we developed a cheap and user friendly tool for bivalve identification through High-Resolution Melting (HRM) analysis. Based on the polymorphism of the nuclear 18S ribosomal RNA gene among 11 orders of bivalve, the 18S-HRM method allowed discriminating species of the Ostreida, Mytilida and Pectinida orders. In this study, this method proved to be a very sensitive tool able to authenticate one species that differed by only one mutation from another one. We applied the method by identifying bivalve species in spat collections from different benthic substrates moored at subtidal and intertidal areas in euhaline conditions. This field survey showed that the settlement of the invasive species Crassostrea gigas was limited vertically to intertidal substrates. In conclusion, the 18S-HRM analysis is a fast, closed-tube method with high resolution and cost effectiveness, and is thus a good alternative method to identify bivalve species. This HRM assay can by applied for species identification of bivalves in the context of ecological management as well as food traceability for bivalves with economical interests. Article in Journal/Newspaper Crassostrea gigas Institut national des sciences de l'Univers: HAL-INSU Marine Biology 164 6 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
ACL Bivalve Oyster Reef Bivalve Species Oyster Species Subtidal Area [SDV]Life Sciences [q-bio] [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL Bivalve Oyster Reef Bivalve Species Oyster Species Subtidal Area [SDV]Life Sciences [q-bio] [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDE.BE]Environmental Sciences/Biodiversity and Ecology Meistertzheim, Anne-Leila Héritier, Laurent Lejart, Morgane High-Resolution Melting of 18S rDNA sequences (18S-HRM) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey |
topic_facet |
ACL Bivalve Oyster Reef Bivalve Species Oyster Species Subtidal Area [SDV]Life Sciences [q-bio] [SDV.BA]Life Sciences [q-bio]/Animal biology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience It is particularly difficult to identify bivalve species at early development stages (larvae and spat) by morphological examination. Targeting genetic marker commonly used for species identification, we developed a cheap and user friendly tool for bivalve identification through High-Resolution Melting (HRM) analysis. Based on the polymorphism of the nuclear 18S ribosomal RNA gene among 11 orders of bivalve, the 18S-HRM method allowed discriminating species of the Ostreida, Mytilida and Pectinida orders. In this study, this method proved to be a very sensitive tool able to authenticate one species that differed by only one mutation from another one. We applied the method by identifying bivalve species in spat collections from different benthic substrates moored at subtidal and intertidal areas in euhaline conditions. This field survey showed that the settlement of the invasive species Crassostrea gigas was limited vertically to intertidal substrates. In conclusion, the 18S-HRM analysis is a fast, closed-tube method with high resolution and cost effectiveness, and is thus a good alternative method to identify bivalve species. This HRM assay can by applied for species identification of bivalves in the context of ecological management as well as food traceability for bivalves with economical interests. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centre de recherches insulaires et observatoire de l'environnement (CRIOBE) Université de Perpignan Via Domitia (UPVD)-École Pratique des Hautes Études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Centre de Formation et de Recherche sur les Environnements Méditérranéens (CEFREM) Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) France Energies Marines Brest |
format |
Article in Journal/Newspaper |
author |
Meistertzheim, Anne-Leila Héritier, Laurent Lejart, Morgane |
author_facet |
Meistertzheim, Anne-Leila Héritier, Laurent Lejart, Morgane |
author_sort |
Meistertzheim, Anne-Leila |
title |
High-Resolution Melting of 18S rDNA sequences (18S-HRM) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey |
title_short |
High-Resolution Melting of 18S rDNA sequences (18S-HRM) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey |
title_full |
High-Resolution Melting of 18S rDNA sequences (18S-HRM) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey |
title_fullStr |
High-Resolution Melting of 18S rDNA sequences (18S-HRM) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey |
title_full_unstemmed |
High-Resolution Melting of 18S rDNA sequences (18S-HRM) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey |
title_sort |
high-resolution melting of 18s rdna sequences (18s-hrm) for discrimination of bivalve’s species at early juvenile stage: application to a spat survey |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://univ-perp.hal.science/hal-01740083 https://doi.org/10.1007/s00227-017-3162-5 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0025-3162 EISSN: 1432-1793 Marine Biology https://univ-perp.hal.science/hal-01740083 Marine Biology, 2017, 164 (6), pp.133. ⟨10.1007/s00227-017-3162-5⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00227-017-3162-5 hal-01740083 https://univ-perp.hal.science/hal-01740083 doi:10.1007/s00227-017-3162-5 |
op_doi |
https://doi.org/10.1007/s00227-017-3162-5 |
container_title |
Marine Biology |
container_volume |
164 |
container_issue |
6 |
_version_ |
1790599428141219840 |