Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition

• Published in: Aquaculture Nutrition
• Main Authors: da Costa, F., Petton, Bruno, Mingant, C., Bougaran, G., Rouxel, C., Quéré, C., Wikfors, G. H., Soudant, Philippe, Robert, R.
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), NOAA National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2016
• Subjects: oyster; Tisochrysis lutea; ACL; lipids; larvae; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Crassostrea gigas
• Online Access: https://hal.science/hal-01483249; https://doi.org/10.1111/anu.12301

International audience Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+ Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93-95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31-33%) and total fatty acids (4.0-4.7 pg cell(-1)). Larval oyster survival and growth rate were positively correlated with 18: 1n-7 and 20: 1n-7, in storage lipids (SL), and negatively related to 14: 0, 18: 1n-9, 20: 1n-9, 20: 4n-6 and trans-22-dehydrocholesterol in membrane lipids (ML). Surprisingly, only the essential fatty acid 20: 5n-3 in SL was correlated positively with larval survival. Correlations suggest that physiological disruption by overabundance of TAG, FFA and certain fatty acids in larvae fed T+ was largely responsible for the poor performance of these larvae. 'High-lipid' strains of microalgae, without regard to qualitative lipid composition, do not always improve bivalve larval performance.