Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions
International audience The Pacific oyster Crassostrea gigas is an important commercial species cultured throughout the world. Oyster production practices often include transfers of animals into new environments that can be stressful, especially at young ages. This study was undertaken to determine i...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2016
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Online Access: | https://hal.science/hal-01483232 https://doi.org/10.1016/j.fsi.2016.02.017 |
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Institut national des sciences de l'Univers: HAL-INSU |
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language |
English |
topic |
ACL Alexandrium Crassostrea gigas Harmful algal blooms hemocyte microbial challenge [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ACL Alexandrium Crassostrea gigas Harmful algal blooms hemocyte microbial challenge [SDE.BE]Environmental Sciences/Biodiversity and Ecology Lassudrie, Malwenn Soudant, Philippe Nicolas, Jean-Louis Miner, Philippe Le Grand, Jacqueline Lambert, Christophe Le Goïc, Nelly Hegaret, Helene Fabioux, Caroline Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions |
topic_facet |
ACL Alexandrium Crassostrea gigas Harmful algal blooms hemocyte microbial challenge [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience The Pacific oyster Crassostrea gigas is an important commercial species cultured throughout the world. Oyster production practices often include transfers of animals into new environments that can be stressful, especially at young ages. This study was undertaken to determine if a toxic Alexandrium bloom, occurring repeatedly in French oyster beds, could modulate juvenile oyster cellular immune responses (i.e. hemocyte variables). We simulated planting on commercial beds by conducting a cohabitation exposure of juvenile, “specific pathogen-free” (SPF) oysters (naïve from the environment) with previously field-exposed oysters to induce interactions with new microorganisms. Indeed, toxic Alexandrium spp. exposures have been reported to modulate bivalve interaction with specific pathogens, as well as physiological and immunological variables in bivalves. In summary, SPF oysters were subjected to an artificial bloom of A. catenella, simultaneously with a cohabitation challenge.ăăExposure to A. catenella, and thus to the paralytic shellfish toxins (PSTs) and extracellular bioactive compounds produced by this alga, induced higher concentration, size, complexity and reactive oxygen species (ROS) production of circulating hemocytes. Challenge by cohabitation with field-exposed oysters also activated these hemocyte responses, suggesting a defense response to new microorganism exposure. These hemocyte responses to cohabitation challenge, however, were partially inhibited by A. catenella exposure, which enhanced hemocyte mortality, suggesting either detrimental effects of the interaction of both stressors on immune capacity, or the implementation of an alternative immune strategy through apoptosis. Indeed, no infection with specific pathogens (herpesvirus OsHV-1 or Vibrio aesturianus) was detected. Additionally, lower PST accumulation in challenged oysters suggests a physiological impairment through alteration of feeding-related processes. Overall, results of this study show that a short-term ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-13-CESA-0019,ACCUTOX,De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire pour l'homme dans son contexte sociétal(2013) |
format |
Article in Journal/Newspaper |
author |
Lassudrie, Malwenn Soudant, Philippe Nicolas, Jean-Louis Miner, Philippe Le Grand, Jacqueline Lambert, Christophe Le Goïc, Nelly Hegaret, Helene Fabioux, Caroline |
author_facet |
Lassudrie, Malwenn Soudant, Philippe Nicolas, Jean-Louis Miner, Philippe Le Grand, Jacqueline Lambert, Christophe Le Goïc, Nelly Hegaret, Helene Fabioux, Caroline |
author_sort |
Lassudrie, Malwenn |
title |
Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions |
title_short |
Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions |
title_full |
Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions |
title_fullStr |
Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions |
title_full_unstemmed |
Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions |
title_sort |
exposure to the toxic dinoflagellate alexandrium catenella modulates juvenile oyster crassostrea gigas hemocyte variables subjected to different biotic conditions |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.science/hal-01483232 https://doi.org/10.1016/j.fsi.2016.02.017 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.science/hal-01483232 Fish and Shellfish Immunology, 2016, 51, pp.104-115. ⟨10.1016/j.fsi.2016.02.017⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2016.02.017 hal-01483232 https://hal.science/hal-01483232 doi:10.1016/j.fsi.2016.02.017 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.02.017 |
container_title |
Fish & Shellfish Immunology |
container_volume |
51 |
container_start_page |
104 |
op_container_end_page |
115 |
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1790599208558919680 |
spelling |
ftinsu:oai:HAL:hal-01483232v1 2024-02-11T10:03:04+01:00 Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions Lassudrie, Malwenn Soudant, Philippe Nicolas, Jean-Louis Miner, Philippe Le Grand, Jacqueline Lambert, Christophe Le Goïc, Nelly Hegaret, Helene Fabioux, Caroline Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-13-CESA-0019,ACCUTOX,De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire pour l'homme dans son contexte sociétal(2013) 2016-04 https://hal.science/hal-01483232 https://doi.org/10.1016/j.fsi.2016.02.017 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2016.02.017 hal-01483232 https://hal.science/hal-01483232 doi:10.1016/j.fsi.2016.02.017 ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.science/hal-01483232 Fish and Shellfish Immunology, 2016, 51, pp.104-115. ⟨10.1016/j.fsi.2016.02.017⟩ ACL Alexandrium Crassostrea gigas Harmful algal blooms hemocyte microbial challenge [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2016 ftinsu https://doi.org/10.1016/j.fsi.2016.02.017 2024-01-24T17:38:23Z International audience The Pacific oyster Crassostrea gigas is an important commercial species cultured throughout the world. Oyster production practices often include transfers of animals into new environments that can be stressful, especially at young ages. This study was undertaken to determine if a toxic Alexandrium bloom, occurring repeatedly in French oyster beds, could modulate juvenile oyster cellular immune responses (i.e. hemocyte variables). We simulated planting on commercial beds by conducting a cohabitation exposure of juvenile, “specific pathogen-free” (SPF) oysters (naïve from the environment) with previously field-exposed oysters to induce interactions with new microorganisms. Indeed, toxic Alexandrium spp. exposures have been reported to modulate bivalve interaction with specific pathogens, as well as physiological and immunological variables in bivalves. In summary, SPF oysters were subjected to an artificial bloom of A. catenella, simultaneously with a cohabitation challenge.ăăExposure to A. catenella, and thus to the paralytic shellfish toxins (PSTs) and extracellular bioactive compounds produced by this alga, induced higher concentration, size, complexity and reactive oxygen species (ROS) production of circulating hemocytes. Challenge by cohabitation with field-exposed oysters also activated these hemocyte responses, suggesting a defense response to new microorganism exposure. These hemocyte responses to cohabitation challenge, however, were partially inhibited by A. catenella exposure, which enhanced hemocyte mortality, suggesting either detrimental effects of the interaction of both stressors on immune capacity, or the implementation of an alternative immune strategy through apoptosis. Indeed, no infection with specific pathogens (herpesvirus OsHV-1 or Vibrio aesturianus) was detected. Additionally, lower PST accumulation in challenged oysters suggests a physiological impairment through alteration of feeding-related processes. Overall, results of this study show that a short-term ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Fish & Shellfish Immunology 51 104 115 |