Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas

00000 ăWOS:000383370300057 International audience This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS wit...

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Published in:Aquaculture
Main Authors: Asmani, Katia, Petton, Bruno, Le Grand, Jacqueline, Mounier, Jérôme, Robert, Rene, Nicolas, Jean-Louis
Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), IFR148 ScInBioS, European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2016
Subjects:
ras
ACL
Online Access:https://hal.science/hal-01483196
https://doi.org/10.1016/j.aquaculture.2016.07.020
id ftinsu:oai:HAL:hal-01483196v1
record_format openpolar
institution Open Polar
collection Institut national des sciences de l'Univers: HAL-INSU
op_collection_id ftinsu
language English
topic aquaculture
bacterial diversity
Bivalve larvae
communities
dynamics
hatchery
marine bivalves
Microbiota
Pyrosequencing
ras
recirculation
systems
temperature
vibrio-parahaemolyticus
ACL
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
spellingShingle aquaculture
bacterial diversity
Bivalve larvae
communities
dynamics
hatchery
marine bivalves
Microbiota
Pyrosequencing
ras
recirculation
systems
temperature
vibrio-parahaemolyticus
ACL
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Asmani, Katia
Petton, Bruno
Le Grand, Jacqueline
Mounier, Jérôme
Robert, Rene
Nicolas, Jean-Louis
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
topic_facet aquaculture
bacterial diversity
Bivalve larvae
communities
dynamics
hatchery
marine bivalves
Microbiota
Pyrosequencing
ras
recirculation
systems
temperature
vibrio-parahaemolyticus
ACL
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
description 00000 ăWOS:000383370300057 International audience This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5% in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 10(5) ml(-1)) and in larvae (10(3) larva(-1)) on day 15. Bacterial assemblages, characterised by 454 pyrosequencing of the V1-V3 region of 16S rRNA, were highly similar (50-65%) for compartments, regardless of rearing system and sampling time, but the compartments were clearly different from one another. At the beginning of rearing, larval microbiota was mostly composed of Proteobacteria (similar to 90%), with 47% Rhodobacteraceae (gamma-Proteobacteria). beta-Proteobacteria, including Pseudoalteromonas, Alteromonas and a few vibrios, declined in the rearing period (25% on day 7 to 9% on day 15). At the end of rearing, colonisation by two members of the Burkholderiales (beta-Proteobacteria), 45% on average on day 15, had decreased overall diversity. Seawater microbiota was more stable with in all batches as one unclassified bacterium present in all batches (27 +/- 7%), 42 OTUs of alpha-Proteobacteria (19 +/- 7%) and 26 of gamma-Proteobacteria (14%). Change was due notably to a species of Cryomorphaceae (Flavobacteria) that reached 15 +/- 7% on day 15. Predatory bacteria, Bdellovivrio spp. and Bacteriovorax spp. were present (3-12%) and could participate in the regulation of bacterial populations. Bacterial assemblages in RS bioreactors remained stable and were mainly composed of ...
author2 Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR)
Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM)
Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM)
Université de Brest (UBO)
IFR148 ScInBioS
European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010)
format Article in Journal/Newspaper
author Asmani, Katia
Petton, Bruno
Le Grand, Jacqueline
Mounier, Jérôme
Robert, Rene
Nicolas, Jean-Louis
author_facet Asmani, Katia
Petton, Bruno
Le Grand, Jacqueline
Mounier, Jérôme
Robert, Rene
Nicolas, Jean-Louis
author_sort Asmani, Katia
title Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
title_short Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
title_full Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
title_fullStr Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
title_full_unstemmed Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
title_sort establishment of microbiota in larval culture of pacific oyster, crassostrea gigas
publisher HAL CCSD
publishDate 2016
url https://hal.science/hal-01483196
https://doi.org/10.1016/j.aquaculture.2016.07.020
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
op_source ISSN: 0044-8486
EISSN: 1873-5622
Aquaculture
https://hal.science/hal-01483196
Aquaculture, 2016, 464, pp.434-444. ⟨10.1016/j.aquaculture.2016.07.020⟩
op_relation info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2016.07.020
info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED
hal-01483196
https://hal.science/hal-01483196
doi:10.1016/j.aquaculture.2016.07.020
op_doi https://doi.org/10.1016/j.aquaculture.2016.07.020
container_title Aquaculture
container_volume 464
container_start_page 434
op_container_end_page 444
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spelling ftinsu:oai:HAL:hal-01483196v1 2024-02-11T10:03:11+01:00 Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jérôme Robert, Rene Nicolas, Jean-Louis Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) IFR148 ScInBioS European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2016-11-01 https://hal.science/hal-01483196 https://doi.org/10.1016/j.aquaculture.2016.07.020 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2016.07.020 info:eu-repo/grantAgreement/EC/FP7/245119/EU/REsearch to improve PROduction of SEED of established and emerging bivalve species in European hatcheries/REPROSEED hal-01483196 https://hal.science/hal-01483196 doi:10.1016/j.aquaculture.2016.07.020 ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-01483196 Aquaculture, 2016, 464, pp.434-444. ⟨10.1016/j.aquaculture.2016.07.020⟩ aquaculture bacterial diversity Bivalve larvae communities dynamics hatchery marine bivalves Microbiota Pyrosequencing ras recirculation systems temperature vibrio-parahaemolyticus ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2016 ftinsu https://doi.org/10.1016/j.aquaculture.2016.07.020 2024-01-24T17:38:23Z 00000 ăWOS:000383370300057 International audience This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5% in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 10(5) ml(-1)) and in larvae (10(3) larva(-1)) on day 15. Bacterial assemblages, characterised by 454 pyrosequencing of the V1-V3 region of 16S rRNA, were highly similar (50-65%) for compartments, regardless of rearing system and sampling time, but the compartments were clearly different from one another. At the beginning of rearing, larval microbiota was mostly composed of Proteobacteria (similar to 90%), with 47% Rhodobacteraceae (gamma-Proteobacteria). beta-Proteobacteria, including Pseudoalteromonas, Alteromonas and a few vibrios, declined in the rearing period (25% on day 7 to 9% on day 15). At the end of rearing, colonisation by two members of the Burkholderiales (beta-Proteobacteria), 45% on average on day 15, had decreased overall diversity. Seawater microbiota was more stable with in all batches as one unclassified bacterium present in all batches (27 +/- 7%), 42 OTUs of alpha-Proteobacteria (19 +/- 7%) and 26 of gamma-Proteobacteria (14%). Change was due notably to a species of Cryomorphaceae (Flavobacteria) that reached 15 +/- 7% on day 15. Predatory bacteria, Bdellovivrio spp. and Bacteriovorax spp. were present (3-12%) and could participate in the regulation of bacterial populations. Bacterial assemblages in RS bioreactors remained stable and were mainly composed of ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Aquaculture 464 434 444