Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1)
International audience Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-rel...
Published in: | Aquaculture Environment Interactions |
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ftinsu:oai:HAL:hal-01483190v1 2024-04-21T08:00:41+00:00 Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) Fuhrmann, Marine Petton, Bruno Quillien, Virgile Faury, Nicole Morga, Benjamin Pernet, Fabrice Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Génétique et Pathologie (LGP) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-12-AGRO-0001,GIGASSAT,Adaptation des écosystèmes ostréicoles au changement global(2012) 2016 https://hal.science/hal-01483190 https://doi.org/10.3354/aei00197 en eng HAL CCSD Inter-reseach science publisher info:eu-repo/semantics/altIdentifier/doi/10.3354/aei00197 hal-01483190 https://hal.science/hal-01483190 doi:10.3354/aei00197 ISSN: 1869-215X Aquaculture Environment Interactions https://hal.science/hal-01483190 Aquaculture Environment Interactions, 2016, 8, pp.543-552. ⟨10.3354/aei00197⟩ herpes-virus Herpesviridae haplosporidium-nelsoni msx Infection mu-var bivalve bivalve mollusks Disease transmission environments host Pacific oysters parasite pcr Risk analysis transmission ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2016 ftinsu https://doi.org/10.3354/aei00197 2024-04-05T00:47:53Z International audience Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35%, 21 degrees C). Water from these tanks was used to infect naive oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35% and (2) oysters acclimated to a salinity of 25%; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25%. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10% was \textgreater 95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field. Article in Journal/Newspaper Crassostrea gigas Institut national des sciences de l'Univers: HAL-INSU Aquaculture Environment Interactions 8 543 552 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
herpes-virus Herpesviridae haplosporidium-nelsoni msx Infection mu-var bivalve bivalve mollusks Disease transmission environments host Pacific oysters parasite pcr Risk analysis transmission ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
herpes-virus Herpesviridae haplosporidium-nelsoni msx Infection mu-var bivalve bivalve mollusks Disease transmission environments host Pacific oysters parasite pcr Risk analysis transmission ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology Fuhrmann, Marine Petton, Bruno Quillien, Virgile Faury, Nicole Morga, Benjamin Pernet, Fabrice Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
topic_facet |
herpes-virus Herpesviridae haplosporidium-nelsoni msx Infection mu-var bivalve bivalve mollusks Disease transmission environments host Pacific oysters parasite pcr Risk analysis transmission ACL [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35%, 21 degrees C). Water from these tanks was used to infect naive oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35% and (2) oysters acclimated to a salinity of 25%; the latter were exposed to OsHV-1 water diluted to a salinity of 10 or 25%. The survival of oysters exposed to OsHV-1 water and acclimated to a salinity of 10% was \textgreater 95%, compared to only 43 to 73% survival in oysters acclimated to higher salinities (Expt 1), reflecting differences in the levels of OsHV-1 DNA and viral gene expression (Expts 1 and 2). However, the survival of their non-acclimated counterparts was only 23% (Expt 2), and the levels of OsHV-1 DNA and the expression of 4 viral genes were low (Expt 1). Thus, OsHV-1 may not have been the ultimate cause of mortality in non-acclimated oysters weakened by a salinity shock. It appears that reducing disease risk by means of low salinity is unlikely in the field. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Génétique et Pathologie (LGP) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-12-AGRO-0001,GIGASSAT,Adaptation des écosystèmes ostréicoles au changement global(2012) |
format |
Article in Journal/Newspaper |
author |
Fuhrmann, Marine Petton, Bruno Quillien, Virgile Faury, Nicole Morga, Benjamin Pernet, Fabrice |
author_facet |
Fuhrmann, Marine Petton, Bruno Quillien, Virgile Faury, Nicole Morga, Benjamin Pernet, Fabrice |
author_sort |
Fuhrmann, Marine |
title |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_short |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_full |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_fullStr |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_full_unstemmed |
Salinity influences disease-induced mortality of the oyster Crassostrea gigas and infectivity of the ostreid herpesvirus 1 (OsHV-1) |
title_sort |
salinity influences disease-induced mortality of the oyster crassostrea gigas and infectivity of the ostreid herpesvirus 1 (oshv-1) |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.science/hal-01483190 https://doi.org/10.3354/aei00197 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1869-215X Aquaculture Environment Interactions https://hal.science/hal-01483190 Aquaculture Environment Interactions, 2016, 8, pp.543-552. ⟨10.3354/aei00197⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3354/aei00197 hal-01483190 https://hal.science/hal-01483190 doi:10.3354/aei00197 |
op_doi |
https://doi.org/10.3354/aei00197 |
container_title |
Aquaculture Environment Interactions |
container_volume |
8 |
container_start_page |
543 |
op_container_end_page |
552 |
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1796941119342247936 |