Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths
International audience We describe a procedure for measuring the thickness and mass of calcite particles that works for most calcite particles <4.5-mu m thick. The calcite particles are observed in cross-polarized light, which enables the light transmitted through the calcite particles to be corr...
Published in: | Nature Protocols |
---|---|
Main Authors: | , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2014
|
Subjects: | |
Online Access: | https://hal.science/hal-01458300 https://doi.org/10.1038/nprot.2014.028 |
id |
ftinsu:oai:HAL:hal-01458300v1 |
---|---|
record_format |
openpolar |
spelling |
ftinsu:oai:HAL:hal-01458300v1 2023-12-17T10:47:58+01:00 Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths Beaufort, Luc, L Barbarin, Nicolas Gally, Yves Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE) Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) 2014-03 https://hal.science/hal-01458300 https://doi.org/10.1038/nprot.2014.028 en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/nprot.2014.028 hal-01458300 https://hal.science/hal-01458300 doi:10.1038/nprot.2014.028 ISSN: 1750-2799 EISSN: 1750-2799 Nature Protocols https://hal.science/hal-01458300 Nature Protocols, 2014, 9 (3), pp.633-642. ⟨10.1038/nprot.2014.028⟩ [SDU.STU]Sciences of the Universe [physics]/Earth Sciences info:eu-repo/semantics/article Journal articles 2014 ftinsu https://doi.org/10.1038/nprot.2014.028 2023-11-22T17:49:27Z International audience We describe a procedure for measuring the thickness and mass of calcite particles that works for most calcite particles <4.5-mu m thick. The calcite particles are observed in cross-polarized light, which enables the light transmitted through the calcite particles to be correlated with their thickness. Three polarizing planes are used to minimize the darkening of crystals at some orientations (black cross). This allows direct measurement of the thickness without recourse to a transfer function. This procedure has been used recently to determine the degree of calcification of coccoliths, which provides an indicator of ocean acidification. It takes only a few minutes per sample, and it is an improvement over the former protocol, which did not allow measurement of the thickness and mass of particles thicker than 1.5 mu m. Article in Journal/Newspaper Ocean acidification Institut national des sciences de l'Univers: HAL-INSU Nature Protocols 9 3 633 642 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
[SDU.STU]Sciences of the Universe [physics]/Earth Sciences |
spellingShingle |
[SDU.STU]Sciences of the Universe [physics]/Earth Sciences Beaufort, Luc, L Barbarin, Nicolas Gally, Yves Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths |
topic_facet |
[SDU.STU]Sciences of the Universe [physics]/Earth Sciences |
description |
International audience We describe a procedure for measuring the thickness and mass of calcite particles that works for most calcite particles <4.5-mu m thick. The calcite particles are observed in cross-polarized light, which enables the light transmitted through the calcite particles to be correlated with their thickness. Three polarizing planes are used to minimize the darkening of crystals at some orientations (black cross). This allows direct measurement of the thickness without recourse to a transfer function. This procedure has been used recently to determine the degree of calcification of coccoliths, which provides an indicator of ocean acidification. It takes only a few minutes per sample, and it is an improvement over the former protocol, which did not allow measurement of the thickness and mass of particles thicker than 1.5 mu m. |
author2 |
Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE) Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Beaufort, Luc, L Barbarin, Nicolas Gally, Yves |
author_facet |
Beaufort, Luc, L Barbarin, Nicolas Gally, Yves |
author_sort |
Beaufort, Luc, L |
title |
Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths |
title_short |
Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths |
title_full |
Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths |
title_fullStr |
Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths |
title_full_unstemmed |
Optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths |
title_sort |
optical measurements to determine the thickness of calcite crystals and the mass of thin carbonate particles such as coccoliths |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.science/hal-01458300 https://doi.org/10.1038/nprot.2014.028 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
ISSN: 1750-2799 EISSN: 1750-2799 Nature Protocols https://hal.science/hal-01458300 Nature Protocols, 2014, 9 (3), pp.633-642. ⟨10.1038/nprot.2014.028⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/nprot.2014.028 hal-01458300 https://hal.science/hal-01458300 doi:10.1038/nprot.2014.028 |
op_doi |
https://doi.org/10.1038/nprot.2014.028 |
container_title |
Nature Protocols |
container_volume |
9 |
container_issue |
3 |
container_start_page |
633 |
op_container_end_page |
642 |
_version_ |
1785572000759021568 |