In vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the Atlantic holothurian Cucumaria frondosa
International audience tHerpes Simplex virus 1 (HSV-1), responsible for the common cold sore, can also lead to serious infectionsin immunocompromised people. Current antiviral chemotherapies face obstacles including the toxicityof therapeutic molecules, interference with normal cellular metabolism,...
Published in: | Process Biochemistry |
---|---|
Main Authors: | , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2015
|
Subjects: | |
Online Access: | https://hal.science/hal-01370479 https://doi.org/10.1016/j.procbio.2015.02.012 |
id |
ftinsu:oai:HAL:hal-01370479v1 |
---|---|
record_format |
openpolar |
spelling |
ftinsu:oai:HAL:hal-01370479v1 2024-02-11T10:03:17+01:00 In vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the Atlantic holothurian Cucumaria frondosa Tripoteau, Ludovic Bedoux, Gilles Gagnon, Jacques Bourgougnon, Nathalie Laboratoire de Biotechnologie et Chimie Marines (LBCM) Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut de Recherche sur les Zones Cotières (IRZC) Université de Moncton 2015-02-23 https://hal.science/hal-01370479 https://doi.org/10.1016/j.procbio.2015.02.012 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2015.02.012 hal-01370479 https://hal.science/hal-01370479 doi:10.1016/j.procbio.2015.02.012 ISSN: 1359-5113 Process Biochemistry https://hal.science/hal-01370479 Process Biochemistry, 2015, 50 (867), ⟨10.1016/j.procbio.2015.02.012⟩ Cucumaria frondosa Enzymatic hydrolysis Herpes simplex virus type 1 Byproducts [CHIM]Chemical Sciences info:eu-repo/semantics/article Journal articles 2015 ftinsu https://doi.org/10.1016/j.procbio.2015.02.012 2024-01-24T17:38:55Z International audience tHerpes Simplex virus 1 (HSV-1), responsible for the common cold sore, can also lead to serious infectionsin immunocompromised people. Current antiviral chemotherapies face obstacles including the toxicityof therapeutic molecules, interference with normal cellular metabolism, genetic variability and the incur-able nature of latent infections. Therefore, the search for new treatments is a public health issue. Marineinvertebrates have held great potential for finding novel antiviral compounds. Little is known, about theantiviral activities of compounds isolated from holothurians. In New Brunswick, holothurian is fishedfor its edible bodywall and muscle, but its processing generates high amounts of byproducts. In vitroevaluation of the anti-HSV-1 activity by cell viability was performed on nine hydrolysates obtained byenzyme-assisted extraction and four solvent extractions from aquapharyngeal bulb and internal organsof Cucumaria frondosa at an MOI of 0.001 ID50/cells. After 72 h, four enzymatic hydrolysates from theaquapharyngeal bulb presented effective antiherpetic activities (EC50= 7.2–15.2 g/mL). After evalua-tion at a higher MOI (0.01 ID50/cells), the most efficient extract (Papain hydrolysate) was fractionatedto identify the active fraction. The fraction superior to 100 kDa showed the highest antiherpetic activity(EC50: 18.2 g/mL). In conclusion, upgrading byproducts of sea cucumber fisheries offers new sources ofbioactive molecules. Article in Journal/Newspaper Cucumaria frondosa Institut national des sciences de l'Univers: HAL-INSU Process Biochemistry 50 5 867 875 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Cucumaria frondosa Enzymatic hydrolysis Herpes simplex virus type 1 Byproducts [CHIM]Chemical Sciences |
spellingShingle |
Cucumaria frondosa Enzymatic hydrolysis Herpes simplex virus type 1 Byproducts [CHIM]Chemical Sciences Tripoteau, Ludovic Bedoux, Gilles Gagnon, Jacques Bourgougnon, Nathalie In vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the Atlantic holothurian Cucumaria frondosa |
topic_facet |
Cucumaria frondosa Enzymatic hydrolysis Herpes simplex virus type 1 Byproducts [CHIM]Chemical Sciences |
description |
International audience tHerpes Simplex virus 1 (HSV-1), responsible for the common cold sore, can also lead to serious infectionsin immunocompromised people. Current antiviral chemotherapies face obstacles including the toxicityof therapeutic molecules, interference with normal cellular metabolism, genetic variability and the incur-able nature of latent infections. Therefore, the search for new treatments is a public health issue. Marineinvertebrates have held great potential for finding novel antiviral compounds. Little is known, about theantiviral activities of compounds isolated from holothurians. In New Brunswick, holothurian is fishedfor its edible bodywall and muscle, but its processing generates high amounts of byproducts. In vitroevaluation of the anti-HSV-1 activity by cell viability was performed on nine hydrolysates obtained byenzyme-assisted extraction and four solvent extractions from aquapharyngeal bulb and internal organsof Cucumaria frondosa at an MOI of 0.001 ID50/cells. After 72 h, four enzymatic hydrolysates from theaquapharyngeal bulb presented effective antiherpetic activities (EC50= 7.2–15.2 g/mL). After evalua-tion at a higher MOI (0.01 ID50/cells), the most efficient extract (Papain hydrolysate) was fractionatedto identify the active fraction. The fraction superior to 100 kDa showed the highest antiherpetic activity(EC50: 18.2 g/mL). In conclusion, upgrading byproducts of sea cucumber fisheries offers new sources ofbioactive molecules. |
author2 |
Laboratoire de Biotechnologie et Chimie Marines (LBCM) Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut de Recherche sur les Zones Cotières (IRZC) Université de Moncton |
format |
Article in Journal/Newspaper |
author |
Tripoteau, Ludovic Bedoux, Gilles Gagnon, Jacques Bourgougnon, Nathalie |
author_facet |
Tripoteau, Ludovic Bedoux, Gilles Gagnon, Jacques Bourgougnon, Nathalie |
author_sort |
Tripoteau, Ludovic |
title |
In vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the Atlantic holothurian Cucumaria frondosa |
title_short |
In vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the Atlantic holothurian Cucumaria frondosa |
title_full |
In vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the Atlantic holothurian Cucumaria frondosa |
title_fullStr |
In vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the Atlantic holothurian Cucumaria frondosa |
title_full_unstemmed |
In vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the Atlantic holothurian Cucumaria frondosa |
title_sort |
in vitro antiviral activities of enzymatic hydrolysates extracted frombyproducts of the atlantic holothurian cucumaria frondosa |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-01370479 https://doi.org/10.1016/j.procbio.2015.02.012 |
genre |
Cucumaria frondosa |
genre_facet |
Cucumaria frondosa |
op_source |
ISSN: 1359-5113 Process Biochemistry https://hal.science/hal-01370479 Process Biochemistry, 2015, 50 (867), ⟨10.1016/j.procbio.2015.02.012⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2015.02.012 hal-01370479 https://hal.science/hal-01370479 doi:10.1016/j.procbio.2015.02.012 |
op_doi |
https://doi.org/10.1016/j.procbio.2015.02.012 |
container_title |
Process Biochemistry |
container_volume |
50 |
container_issue |
5 |
container_start_page |
867 |
op_container_end_page |
875 |
_version_ |
1790599505224138752 |