Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure.
International audience The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of sha...
Published in: | Cytometry Part A |
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Online Access: | https://hal.univ-brest.fr/hal-01096877 https://doi.org/10.1002/cyto.a.22577 |
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ftinsu:oai:HAL:hal-01096877v1 2024-02-11T10:03:05+01:00 Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure. Le Goïc, Nelly Hegaret, Helene Boulais, Myrina Béguel, Jean-Philippe Lambert, Christophe Fabioux, Caroline Soudant, Philippe Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie des Invertébrés (LPI) Physiologie Fonctionnelle des Organismes Marins (PFOM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2014-12 https://hal.univ-brest.fr/hal-01096877 https://doi.org/10.1002/cyto.a.22577 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1002/cyto.a.22577 info:eu-repo/semantics/altIdentifier/pmid/25327519 hal-01096877 https://hal.univ-brest.fr/hal-01096877 doi:10.1002/cyto.a.22577 PUBMED: 25327519 ISSN: 1552-4922 EISSN: 1552-4930 Cytometry Part A https://hal.univ-brest.fr/hal-01096877 Cytometry Part A, 2014, 85A (12), pp.1049-1056. ⟨10.1002/cyto.a.22577⟩ viability Pacific oyster ROS production gamete quality flow cytometry ecotoxicology ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2014 ftinsu https://doi.org/10.1002/cyto.a.22577 2024-01-24T17:40:20Z International audience The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Cytometry Part A 85 12 1049 1056 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
viability Pacific oyster ROS production gamete quality flow cytometry ecotoxicology ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
viability Pacific oyster ROS production gamete quality flow cytometry ecotoxicology ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Le Goïc, Nelly Hegaret, Helene Boulais, Myrina Béguel, Jean-Philippe Lambert, Christophe Fabioux, Caroline Soudant, Philippe Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure. |
topic_facet |
viability Pacific oyster ROS production gamete quality flow cytometry ecotoxicology ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physiologie des Invertébrés (LPI) Physiologie Fonctionnelle des Organismes Marins (PFOM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Le Goïc, Nelly Hegaret, Helene Boulais, Myrina Béguel, Jean-Philippe Lambert, Christophe Fabioux, Caroline Soudant, Philippe |
author_facet |
Le Goïc, Nelly Hegaret, Helene Boulais, Myrina Béguel, Jean-Philippe Lambert, Christophe Fabioux, Caroline Soudant, Philippe |
author_sort |
Le Goïc, Nelly |
title |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure. |
title_short |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure. |
title_full |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure. |
title_fullStr |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure. |
title_full_unstemmed |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure. |
title_sort |
flow cytometric assessment of morphology, viability, and production of reactive oxygen species of crassostrea gigas oocytes. application to toxic dinoflagellate (alexandrium minutum) exposure. |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.univ-brest.fr/hal-01096877 https://doi.org/10.1002/cyto.a.22577 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1552-4922 EISSN: 1552-4930 Cytometry Part A https://hal.univ-brest.fr/hal-01096877 Cytometry Part A, 2014, 85A (12), pp.1049-1056. ⟨10.1002/cyto.a.22577⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/cyto.a.22577 info:eu-repo/semantics/altIdentifier/pmid/25327519 hal-01096877 https://hal.univ-brest.fr/hal-01096877 doi:10.1002/cyto.a.22577 PUBMED: 25327519 |
op_doi |
https://doi.org/10.1002/cyto.a.22577 |
container_title |
Cytometry Part A |
container_volume |
85 |
container_issue |
12 |
container_start_page |
1049 |
op_container_end_page |
1056 |
_version_ |
1790599229252567040 |