Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas

• Main Author: Meistertzheim, Anne-Leila
• Other Authors: Centre de formation et de recherche en environnement marin (CEFREM), Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
• Format: Book Part
• Language: English
• Published: HAL CCSD 2013
• Subjects: Crassostrea gigas; ELISA; Gonad; Heat Shock Proteins; Maturation stage; Metallothioneins; Repartition area; Reproduction; Sex; Temperature; Thermal limit; [SDV]Life Sciences [q-bio]; Pacific; Pacific oyster
• Online Access: https://hal.univ-brest.fr/hal-01025439

International audience Heat Shock Protein 70 (HSP70) are generalist stress proteins expressed in response to numerous environmental stresses. However, the baselines of these constitutive proteins are still unknown in many organisms and in particular marine intertidal species such as the Pacific oyster, Crassostrea gigas. I demonstrate that levels of heat shock proteins (HSP) and other stress proteins (metallothioneins, MTs) quantified by ELISA, remained similar in gills, mantle and digestive gland between oysters inhabiting cold and hot sites. In contrast, endogenous HSPs and MTs levels in gonad changed significantly during gametogenesis. In female gonads, the constitutive form of HSP70 and the MTs increased from immature to mature stages (about more than 3-fold) and decreased after spawning. In male gonads, the same expression patterns were observed, whereas protein levels were lower and decreased once fully mature. I hypothesize that the high level of stress proteins in eggs may increase survival of oyster progeny