Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa
International audience Abstract - The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study ai...
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Online Access: | https://hal.science/hal-00856292 https://hal.science/hal-00856292/document https://hal.science/hal-00856292/file/Le_Goic_2013.pdf https://doi.org/10.1051/alr/2013047 |
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ftinsu:oai:HAL:hal-00856292v1 2024-02-11T10:03:16+01:00 Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa Le Goïc, Nelly Hegaret, Helene Fabioux, Caroline Miner, Philippe Suquet, Marc Lambert, Christophe Soudant, Philippe Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2013-08-05 https://hal.science/hal-00856292 https://hal.science/hal-00856292/document https://hal.science/hal-00856292/file/Le_Goic_2013.pdf https://doi.org/10.1051/alr/2013047 en eng HAL CCSD EDP Sciences info:eu-repo/semantics/altIdentifier/doi/10.1051/alr/2013047 hal-00856292 https://hal.science/hal-00856292 https://hal.science/hal-00856292/document https://hal.science/hal-00856292/file/Le_Goic_2013.pdf doi:10.1051/alr/2013047 info:eu-repo/semantics/OpenAccess ISSN: 0990-7440 EISSN: 1765-2952 Aquatic Living Resources https://hal.science/hal-00856292 Aquatic Living Resources, 2013, 26 (3), pp.221-228. ⟨10.1051/alr/2013047⟩ [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftinsu https://doi.org/10.1051/alr/2013047 2024-01-24T17:22:45Z International audience Abstract - The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only penetrates cells with intact membranes. Cell mortality was measured with propidium iodide (PI), which penetrates cells with membrane damage. Mitochondrial membrane potential, used as an estimate of mitochondrial function, was measured using JC-1 dye, which selectively enters into mitochondria and reversibly changes colour from green to orange as the membrane potential increases. To assess the effect of toxic algae on oyster sperm, broodstock (ripe oysters) were fed toxic (A. catenella) or non toxic (Heterocapsa triquetra) dinoflagellates at 250 cellml−1 for 9 days. After this exposure period, mature oysters were stripped and cellular responses of sperm analysed. Average DNA staining, as measured by SYBR-14, appeared lower and more variable in gametes from A. catenella-exposed oysters than in those from control oysters fed H. triquetra. Additionally, mitochondrial membrane potential of sperm from A. catenella-exposed oysters was significantly higher (1.5 fold) than that of sperm produced by oysters fed H. triquetra. Both the increase of mitochondrial membrane potential and the modification of DNA structure can be expected to impact spermatozoa ability to fertilize oocytes and could thus impact related reproductive processes. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Aquatic Living Resources 26 3 221 228 |
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Open Polar |
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Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
spellingShingle |
[SDV]Life Sciences [q-bio] Le Goïc, Nelly Hegaret, Helene Fabioux, Caroline Miner, Philippe Suquet, Marc Lambert, Christophe Soudant, Philippe Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
International audience Abstract - The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only penetrates cells with intact membranes. Cell mortality was measured with propidium iodide (PI), which penetrates cells with membrane damage. Mitochondrial membrane potential, used as an estimate of mitochondrial function, was measured using JC-1 dye, which selectively enters into mitochondria and reversibly changes colour from green to orange as the membrane potential increases. To assess the effect of toxic algae on oyster sperm, broodstock (ripe oysters) were fed toxic (A. catenella) or non toxic (Heterocapsa triquetra) dinoflagellates at 250 cellml−1 for 9 days. After this exposure period, mature oysters were stripped and cellular responses of sperm analysed. Average DNA staining, as measured by SYBR-14, appeared lower and more variable in gametes from A. catenella-exposed oysters than in those from control oysters fed H. triquetra. Additionally, mitochondrial membrane potential of sperm from A. catenella-exposed oysters was significantly higher (1.5 fold) than that of sperm produced by oysters fed H. triquetra. Both the increase of mitochondrial membrane potential and the modification of DNA structure can be expected to impact spermatozoa ability to fertilize oocytes and could thus impact related reproductive processes. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Le Goïc, Nelly Hegaret, Helene Fabioux, Caroline Miner, Philippe Suquet, Marc Lambert, Christophe Soudant, Philippe |
author_facet |
Le Goïc, Nelly Hegaret, Helene Fabioux, Caroline Miner, Philippe Suquet, Marc Lambert, Christophe Soudant, Philippe |
author_sort |
Le Goïc, Nelly |
title |
Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa |
title_short |
Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa |
title_full |
Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa |
title_fullStr |
Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa |
title_full_unstemmed |
Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa |
title_sort |
impact of the toxic dinoflagellate alexandrium catenella on pacific oyster reproductive output: application of flow cytometry assays on spermatozoa |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.science/hal-00856292 https://hal.science/hal-00856292/document https://hal.science/hal-00856292/file/Le_Goic_2013.pdf https://doi.org/10.1051/alr/2013047 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0990-7440 EISSN: 1765-2952 Aquatic Living Resources https://hal.science/hal-00856292 Aquatic Living Resources, 2013, 26 (3), pp.221-228. ⟨10.1051/alr/2013047⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1051/alr/2013047 hal-00856292 https://hal.science/hal-00856292 https://hal.science/hal-00856292/document https://hal.science/hal-00856292/file/Le_Goic_2013.pdf doi:10.1051/alr/2013047 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1051/alr/2013047 |
container_title |
Aquatic Living Resources |
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26 |
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3 |
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221 |
op_container_end_page |
228 |
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1790599467502665728 |