Integrative study of physiological changes associated with bacterial infection in pacific oyster larvae.
International audience BACKGROUND: Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at lar...
Published in: | PLoS ONE |
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Main Authors: | , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2013
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Online Access: | https://hal.univ-brest.fr/hal-00833831 https://hal.univ-brest.fr/hal-00833831/document https://hal.univ-brest.fr/hal-00833831/file/PLOS-Genard.pdf https://doi.org/10.1371/journal.pone.0064534 |
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Open Polar |
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Institut national des sciences de l'Univers: HAL-INSU |
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ftinsu |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Genard, Bertrand Miner, Philippe Nicolas, Jean-Louis Moraga, Dario Boudry, Pierre Pernet, Fabrice Tremblay, Réjean Integrative study of physiological changes associated with bacterial infection in pacific oyster larvae. |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience BACKGROUND: Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus. FINDINGS: Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase of larvae mortality compared with controls, (2) declined feeding activity, which resulted in energy status changes (i.e. reserve consumption, β-oxidation, decline of metabolic rate), (3) fatty acid remodeling of polar lipids (changes in phosphatidylinositol and lysophosphatidylcholine composition', non-methylene-interrupted fatty acids accumulation, lower content of major C20 polyunsaturated fatty acids as well as activation of desaturases, phospholipase and lipoxygenase), (4) activation of antioxidant defenses (catalase, superoxide dismutase, peroxiredoxin) and cytoprotective processes (heat shock protein 70, pernin), and (5) activation of the immune response (non-self recognition, NF-κκ signaling pathway, haematopoiesis, eiconosoids and lysophosphatidyl acid synthesis, inhibitor of metalloproteinase and antimicrobial peptides). CONCLUSION: Overall, our results allowed us to propose an integrative view of changes induced by a bacterial infection in Pacific oyster larvae, opening new perspectives on the response of marine bivalve larvae to infections. |
author2 |
Institut des Sciences de la MER de Rimouski (ISMER) Université du Québec à Rimouski (UQAR) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Environnement Ressources Languedoc Roussillon (LERLR) Unité Littoral (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut des Sciences de la Mer |
format |
Article in Journal/Newspaper |
author |
Genard, Bertrand Miner, Philippe Nicolas, Jean-Louis Moraga, Dario Boudry, Pierre Pernet, Fabrice Tremblay, Réjean |
author_facet |
Genard, Bertrand Miner, Philippe Nicolas, Jean-Louis Moraga, Dario Boudry, Pierre Pernet, Fabrice Tremblay, Réjean |
author_sort |
Genard, Bertrand |
title |
Integrative study of physiological changes associated with bacterial infection in pacific oyster larvae. |
title_short |
Integrative study of physiological changes associated with bacterial infection in pacific oyster larvae. |
title_full |
Integrative study of physiological changes associated with bacterial infection in pacific oyster larvae. |
title_fullStr |
Integrative study of physiological changes associated with bacterial infection in pacific oyster larvae. |
title_full_unstemmed |
Integrative study of physiological changes associated with bacterial infection in pacific oyster larvae. |
title_sort |
integrative study of physiological changes associated with bacterial infection in pacific oyster larvae. |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.univ-brest.fr/hal-00833831 https://hal.univ-brest.fr/hal-00833831/document https://hal.univ-brest.fr/hal-00833831/file/PLOS-Genard.pdf https://doi.org/10.1371/journal.pone.0064534 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.univ-brest.fr/hal-00833831 PLoS ONE, 2013, 8 (5), pp.e64534. ⟨10.1371/journal.pone.0064534⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0064534 info:eu-repo/semantics/altIdentifier/pmid/23704993 hal-00833831 https://hal.univ-brest.fr/hal-00833831 https://hal.univ-brest.fr/hal-00833831/document https://hal.univ-brest.fr/hal-00833831/file/PLOS-Genard.pdf doi:10.1371/journal.pone.0064534 PUBMED: 23704993 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0064534 |
container_title |
PLoS ONE |
container_volume |
8 |
container_issue |
5 |
container_start_page |
e64534 |
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1790599409048748032 |
spelling |
ftinsu:oai:HAL:hal-00833831v1 2024-02-11T10:03:13+01:00 Integrative study of physiological changes associated with bacterial infection in pacific oyster larvae. Genard, Bertrand Miner, Philippe Nicolas, Jean-Louis Moraga, Dario Boudry, Pierre Pernet, Fabrice Tremblay, Réjean Institut des Sciences de la MER de Rimouski (ISMER) Université du Québec à Rimouski (UQAR) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Environnement Ressources Languedoc Roussillon (LERLR) Unité Littoral (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Institut des Sciences de la Mer 2013 https://hal.univ-brest.fr/hal-00833831 https://hal.univ-brest.fr/hal-00833831/document https://hal.univ-brest.fr/hal-00833831/file/PLOS-Genard.pdf https://doi.org/10.1371/journal.pone.0064534 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0064534 info:eu-repo/semantics/altIdentifier/pmid/23704993 hal-00833831 https://hal.univ-brest.fr/hal-00833831 https://hal.univ-brest.fr/hal-00833831/document https://hal.univ-brest.fr/hal-00833831/file/PLOS-Genard.pdf doi:10.1371/journal.pone.0064534 PUBMED: 23704993 info:eu-repo/semantics/OpenAccess ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.univ-brest.fr/hal-00833831 PLoS ONE, 2013, 8 (5), pp.e64534. ⟨10.1371/journal.pone.0064534⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2013 ftinsu https://doi.org/10.1371/journal.pone.0064534 2024-01-24T17:22:55Z International audience BACKGROUND: Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus. FINDINGS: Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase of larvae mortality compared with controls, (2) declined feeding activity, which resulted in energy status changes (i.e. reserve consumption, β-oxidation, decline of metabolic rate), (3) fatty acid remodeling of polar lipids (changes in phosphatidylinositol and lysophosphatidylcholine composition', non-methylene-interrupted fatty acids accumulation, lower content of major C20 polyunsaturated fatty acids as well as activation of desaturases, phospholipase and lipoxygenase), (4) activation of antioxidant defenses (catalase, superoxide dismutase, peroxiredoxin) and cytoprotective processes (heat shock protein 70, pernin), and (5) activation of the immune response (non-self recognition, NF-κκ signaling pathway, haematopoiesis, eiconosoids and lysophosphatidyl acid synthesis, inhibitor of metalloproteinase and antimicrobial peptides). CONCLUSION: Overall, our results allowed us to propose an integrative view of changes induced by a bacterial infection in Pacific oyster larvae, opening new perspectives on the response of marine bivalve larvae to infections. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific PLoS ONE 8 5 e64534 |