Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.
International audience As oxygen concentrations in marine coastal habitats can fluctuate rapidly and drastically, sessile marine organisms such as the oyster Crassostrea gigas can experience marked and rapid oxygen variations. In this study, we investigated the responses of oyster gill mitochondria...
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ftinsu:oai:HAL:hal-00823259v1 2024-02-11T10:03:08+01:00 Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas. Sussarellu, Rossana Dudognon, Tony Fabioux, Caroline Soudant, Philippe Moraga, Dario Kraffe, Edouard Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2013-05-01 https://hal.univ-brest.fr/hal-00823259 https://doi.org/10.1242/jeb.075879 en eng HAL CCSD The Company of Biologists info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.075879 info:eu-repo/semantics/altIdentifier/pmid/23307802 hal-00823259 https://hal.univ-brest.fr/hal-00823259 doi:10.1242/jeb.075879 PUBMED: 23307802 ISSN: 0022-0949 EISSN: 1477-9145 Journal of Experimental Biology https://hal.univ-brest.fr/hal-00823259 Journal of Experimental Biology, 2013, 216 (Pt 9), pp.1561-9. ⟨10.1242/jeb.075879⟩ hypoxia re-oxygenation oyster mitochondria respiration alternative oxidase [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2013 ftinsu https://doi.org/10.1242/jeb.075879 2024-01-24T17:23:01Z International audience As oxygen concentrations in marine coastal habitats can fluctuate rapidly and drastically, sessile marine organisms such as the oyster Crassostrea gigas can experience marked and rapid oxygen variations. In this study, we investigated the responses of oyster gill mitochondria to short-term hypoxia (3 and 12 h, at 1.7 mg O2 l(-1)) and subsequent re-oxygenation. Mitochondrial respiratory rates (states 3 and 4 stimulated by glutamate) and phosphorylation efficiency [respiratory control ratio (RCR) and the relationship between ADP and oxygen consumption (ADP/O)] were measured. Cytochrome c oxidase (CCO) activity and cytochrome concentrations (a, b, c1 and c) were measured to investigate the rearrangements of respiratory chain subunits. The potential implication of an alternative oxidase (AOX) was investigated using an inhibitor of the respiratory chain (antimycin A) and through gene expression analysis in gills and digestive gland. Results indicate a downregulation of mitochondrial capacity, with 60% inhibition of respiratory rates after 12 h of hypoxia. RCR remained stable, while ADP/O increased after 12 h of hypoxia and 1 h of re-oxygenation, suggesting increased phosphorylation efficiency. CCO showed a fast and remarkable increase of its catalytic activity only after 3 h of hypoxia. AOX mRNA levels showed similar patterns in gills and digestive gland, and were upregulated after 12 and 24 h of hypoxia and during re-oxygenation. Results suggest a set of controls regulating mitochondrial functions in response to oxygen fluctuations, and demonstrate the fast and extreme plasticity of oyster mitochondria in response to oxygen variations. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Journal of Experimental Biology |
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Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
hypoxia re-oxygenation oyster mitochondria respiration alternative oxidase [SDE]Environmental Sciences |
spellingShingle |
hypoxia re-oxygenation oyster mitochondria respiration alternative oxidase [SDE]Environmental Sciences Sussarellu, Rossana Dudognon, Tony Fabioux, Caroline Soudant, Philippe Moraga, Dario Kraffe, Edouard Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas. |
topic_facet |
hypoxia re-oxygenation oyster mitochondria respiration alternative oxidase [SDE]Environmental Sciences |
description |
International audience As oxygen concentrations in marine coastal habitats can fluctuate rapidly and drastically, sessile marine organisms such as the oyster Crassostrea gigas can experience marked and rapid oxygen variations. In this study, we investigated the responses of oyster gill mitochondria to short-term hypoxia (3 and 12 h, at 1.7 mg O2 l(-1)) and subsequent re-oxygenation. Mitochondrial respiratory rates (states 3 and 4 stimulated by glutamate) and phosphorylation efficiency [respiratory control ratio (RCR) and the relationship between ADP and oxygen consumption (ADP/O)] were measured. Cytochrome c oxidase (CCO) activity and cytochrome concentrations (a, b, c1 and c) were measured to investigate the rearrangements of respiratory chain subunits. The potential implication of an alternative oxidase (AOX) was investigated using an inhibitor of the respiratory chain (antimycin A) and through gene expression analysis in gills and digestive gland. Results indicate a downregulation of mitochondrial capacity, with 60% inhibition of respiratory rates after 12 h of hypoxia. RCR remained stable, while ADP/O increased after 12 h of hypoxia and 1 h of re-oxygenation, suggesting increased phosphorylation efficiency. CCO showed a fast and remarkable increase of its catalytic activity only after 3 h of hypoxia. AOX mRNA levels showed similar patterns in gills and digestive gland, and were upregulated after 12 and 24 h of hypoxia and during re-oxygenation. Results suggest a set of controls regulating mitochondrial functions in response to oxygen fluctuations, and demonstrate the fast and extreme plasticity of oyster mitochondria in response to oxygen variations. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Sussarellu, Rossana Dudognon, Tony Fabioux, Caroline Soudant, Philippe Moraga, Dario Kraffe, Edouard |
author_facet |
Sussarellu, Rossana Dudognon, Tony Fabioux, Caroline Soudant, Philippe Moraga, Dario Kraffe, Edouard |
author_sort |
Sussarellu, Rossana |
title |
Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas. |
title_short |
Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas. |
title_full |
Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas. |
title_fullStr |
Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas. |
title_full_unstemmed |
Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas. |
title_sort |
rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the pacific oyster, crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.univ-brest.fr/hal-00823259 https://doi.org/10.1242/jeb.075879 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-0949 EISSN: 1477-9145 Journal of Experimental Biology https://hal.univ-brest.fr/hal-00823259 Journal of Experimental Biology, 2013, 216 (Pt 9), pp.1561-9. ⟨10.1242/jeb.075879⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.075879 info:eu-repo/semantics/altIdentifier/pmid/23307802 hal-00823259 https://hal.univ-brest.fr/hal-00823259 doi:10.1242/jeb.075879 PUBMED: 23307802 |
op_doi |
https://doi.org/10.1242/jeb.075879 |
container_title |
Journal of Experimental Biology |
_version_ |
1790599304489992192 |