Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): comparison with salmonid species and analysis of CpG methylation.
International audience Marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (HUFA) in comparison to strict freshwater and anadromous species. It is admitted that the Delta(6) desaturase (FADS2) is a key enzyme in the HUFA biosynthetic pathway. We investigated by qu...
Published in: | Marine Genomics |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hal.univ-brest.fr/hal-00788353 https://doi.org/10.1016/j.margen.2011.08.003 |
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ftinsu:oai:HAL:hal-00788353v1 2024-02-11T10:02:12+01:00 Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): comparison with salmonid species and analysis of CpG methylation. Geay, Florian Zambonino-Infante, Jose-Luis Reinhardt, Richard Kuhl, Heiner Santigosa, Ester Cahu, Chantal Mazurais, David Physiologie Fonctionnelle des Organismes Marins (PFOM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Max-Planck-Institut für Informatik (MPII) Max-Planck-Gesellschaft Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2012-03 https://hal.univ-brest.fr/hal-00788353 https://doi.org/10.1016/j.margen.2011.08.003 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1016/j.margen.2011.08.003 info:eu-repo/semantics/altIdentifier/pmid/22325717 hal-00788353 https://hal.univ-brest.fr/hal-00788353 doi:10.1016/j.margen.2011.08.003 PUBMED: 22325717 Mar Genomics https://hal.univ-brest.fr/hal-00788353 Mar Genomics, 2012, 5, pp.7-13. ⟨10.1016/j.margen.2011.08.003⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftinsu https://doi.org/10.1016/j.margen.2011.08.003 2024-01-24T17:23:14Z International audience Marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (HUFA) in comparison to strict freshwater and anadromous species. It is admitted that the Delta(6) desaturase (FADS2) is a key enzyme in the HUFA biosynthetic pathway. We investigated by quantitative PCR the relative amounts of FADS2 mRNA in European sea bass (Dicentrarchus labrax) in comparison with a salmonid species, the rainbow trout (Oncorhynchus mykiss L.). The analysis of the expression data was performed regarding the difference of the characteristics of a critical fragment of the fads2 gene promoter between sea bass and Atlantic salmon. The lower level of fads2 gene expression observed in sea bass suggested that fads2 gene putative promoter, which exhibited an E-box like Sterol Regulatory Element (SRE) site but lacked a Sp1 site, is less active in this marine species. The cytosine methylation of CpG sites in the putative promoter region including E-box like SRE and NF-Y binding sites of sea bass fads2 gene was also investigated following a nutritional conditioning of larvae. However, no significant difference of CpG methylation could be found for any of the 28 CpGs analysed between larvae fed diet with high or low HUFA contents. In conclusion, the present data revealed lower constitutive expression of the fads2 gene possibly related to different characteristics of gene promoter in sea bass in comparison with salmonid species, and indicated that long-term conditioning of fads2 gene expression did not influence the methylation of the gene promoter at potential SRE binding site. Article in Journal/Newspaper Atlantic salmon Institut national des sciences de l'Univers: HAL-INSU Marine Genomics 5 7 13 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Geay, Florian Zambonino-Infante, Jose-Luis Reinhardt, Richard Kuhl, Heiner Santigosa, Ester Cahu, Chantal Mazurais, David Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): comparison with salmonid species and analysis of CpG methylation. |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience Marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (HUFA) in comparison to strict freshwater and anadromous species. It is admitted that the Delta(6) desaturase (FADS2) is a key enzyme in the HUFA biosynthetic pathway. We investigated by quantitative PCR the relative amounts of FADS2 mRNA in European sea bass (Dicentrarchus labrax) in comparison with a salmonid species, the rainbow trout (Oncorhynchus mykiss L.). The analysis of the expression data was performed regarding the difference of the characteristics of a critical fragment of the fads2 gene promoter between sea bass and Atlantic salmon. The lower level of fads2 gene expression observed in sea bass suggested that fads2 gene putative promoter, which exhibited an E-box like Sterol Regulatory Element (SRE) site but lacked a Sp1 site, is less active in this marine species. The cytosine methylation of CpG sites in the putative promoter region including E-box like SRE and NF-Y binding sites of sea bass fads2 gene was also investigated following a nutritional conditioning of larvae. However, no significant difference of CpG methylation could be found for any of the 28 CpGs analysed between larvae fed diet with high or low HUFA contents. In conclusion, the present data revealed lower constitutive expression of the fads2 gene possibly related to different characteristics of gene promoter in sea bass in comparison with salmonid species, and indicated that long-term conditioning of fads2 gene expression did not influence the methylation of the gene promoter at potential SRE binding site. |
author2 |
Physiologie Fonctionnelle des Organismes Marins (PFOM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Max-Planck-Institut für Informatik (MPII) Max-Planck-Gesellschaft Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Geay, Florian Zambonino-Infante, Jose-Luis Reinhardt, Richard Kuhl, Heiner Santigosa, Ester Cahu, Chantal Mazurais, David |
author_facet |
Geay, Florian Zambonino-Infante, Jose-Luis Reinhardt, Richard Kuhl, Heiner Santigosa, Ester Cahu, Chantal Mazurais, David |
author_sort |
Geay, Florian |
title |
Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): comparison with salmonid species and analysis of CpG methylation. |
title_short |
Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): comparison with salmonid species and analysis of CpG methylation. |
title_full |
Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): comparison with salmonid species and analysis of CpG methylation. |
title_fullStr |
Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): comparison with salmonid species and analysis of CpG methylation. |
title_full_unstemmed |
Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): comparison with salmonid species and analysis of CpG methylation. |
title_sort |
characteristics of fads2 gene expression and putative promoter in european sea bass (dicentrarchus labrax): comparison with salmonid species and analysis of cpg methylation. |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00788353 https://doi.org/10.1016/j.margen.2011.08.003 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Mar Genomics https://hal.univ-brest.fr/hal-00788353 Mar Genomics, 2012, 5, pp.7-13. ⟨10.1016/j.margen.2011.08.003⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.margen.2011.08.003 info:eu-repo/semantics/altIdentifier/pmid/22325717 hal-00788353 https://hal.univ-brest.fr/hal-00788353 doi:10.1016/j.margen.2011.08.003 PUBMED: 22325717 |
op_doi |
https://doi.org/10.1016/j.margen.2011.08.003 |
container_title |
Marine Genomics |
container_volume |
5 |
container_start_page |
7 |
op_container_end_page |
13 |
_version_ |
1790598117435899904 |