Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions.
International audience The molecular response to hypoxia stress in aquatic invertebrates remains relatively unknown. In this study, we investigated the response of the Pacific oyster Crassostrea gigas to hypoxia under experimental conditions and focused on the analysis of the differential expression...
Published in: | FEBS Journal |
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Main Authors: | , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2005
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Online Access: | https://hal.univ-brest.fr/hal-00750438 https://doi.org/10.1111/j.1742-4658.2005.04960.x |
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Open Polar |
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Institut national des sciences de l'Univers: HAL-INSU |
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ftinsu |
language |
English |
topic |
MESH: Animals MESH: Anoxia MESH: Gene Expression Regulation MESH: HSP70 Heat-Shock Proteins MESH: Metallothionein MESH: Ostreidae MESH: Pacific Ocean [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
spellingShingle |
MESH: Animals MESH: Anoxia MESH: Gene Expression Regulation MESH: HSP70 Heat-Shock Proteins MESH: Metallothionein MESH: Ostreidae MESH: Pacific Ocean [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] David, Elise Tanguy, Arnaud Pichavant, Karine Moraga, Dario Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. |
topic_facet |
MESH: Animals MESH: Anoxia MESH: Gene Expression Regulation MESH: HSP70 Heat-Shock Proteins MESH: Metallothionein MESH: Ostreidae MESH: Pacific Ocean [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
description |
International audience The molecular response to hypoxia stress in aquatic invertebrates remains relatively unknown. In this study, we investigated the response of the Pacific oyster Crassostrea gigas to hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific genes associated with hypoxia response. A suppression subtractive hybridization method was used to identify specific hypoxia up- and downregulated genes, in gills, mantle and digestive gland, after 7-10 days and 24 days of exposure. This method revealed 616 different sequences corresponding to 12 major physiological functions. The expression of eight potentially regulated genes was analysed by RT-PCR in different tissues at different sampling times over the time course of hypoxia. These genes are implicated in different physiological pathways such as respiration (carbonic anhydrase), carbohydrate metabolism (glycogen phosphorylase), lipid metabolism (delta-9 desaturase), oxidative metabolism and the immune system (glutathione peroxidase), protein regulation (BTF3, transcription factor), nucleic acid regulation (myc homologue), metal sequestration (putative metallothionein) and stress response (heat shock protein 70). Stress proteins (metallothioneins and heat shock proteins) were also quantified. This study contributes to the characterization of many potential genetic markers that could be used in future environmental monitoring, and could lead to explore new mechanisms of stress tolerance in marine mollusc species. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Optimisation des régulations physiologiques (ORPHY (EA 4324)) Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM) Université de Brest (UBO)-Université de Brest (UBO) |
format |
Article in Journal/Newspaper |
author |
David, Elise Tanguy, Arnaud Pichavant, Karine Moraga, Dario |
author_facet |
David, Elise Tanguy, Arnaud Pichavant, Karine Moraga, Dario |
author_sort |
David, Elise |
title |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. |
title_short |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. |
title_full |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. |
title_fullStr |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. |
title_full_unstemmed |
Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. |
title_sort |
response of the pacific oyster crassostrea gigas to hypoxia exposure under experimental conditions. |
publisher |
HAL CCSD |
publishDate |
2005 |
url |
https://hal.univ-brest.fr/hal-00750438 https://doi.org/10.1111/j.1742-4658.2005.04960.x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1742-464X EISSN: 1742-4658 FEBS Journal https://hal.univ-brest.fr/hal-00750438 FEBS Journal, 2005, 272 (21), pp.5635-52. ⟨10.1111/j.1742-4658.2005.04960.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1742-4658.2005.04960.x info:eu-repo/semantics/altIdentifier/pmid/16262701 hal-00750438 https://hal.univ-brest.fr/hal-00750438 doi:10.1111/j.1742-4658.2005.04960.x PUBMED: 16262701 |
op_doi |
https://doi.org/10.1111/j.1742-4658.2005.04960.x |
container_title |
FEBS Journal |
container_volume |
272 |
container_issue |
21 |
container_start_page |
5635 |
op_container_end_page |
5652 |
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1790599280632791040 |
spelling |
ftinsu:oai:HAL:hal-00750438v1 2024-02-11T10:03:07+01:00 Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. David, Elise Tanguy, Arnaud Pichavant, Karine Moraga, Dario Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Optimisation des régulations physiologiques (ORPHY (EA 4324)) Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM) Université de Brest (UBO)-Université de Brest (UBO) 2005-11 https://hal.univ-brest.fr/hal-00750438 https://doi.org/10.1111/j.1742-4658.2005.04960.x en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1742-4658.2005.04960.x info:eu-repo/semantics/altIdentifier/pmid/16262701 hal-00750438 https://hal.univ-brest.fr/hal-00750438 doi:10.1111/j.1742-4658.2005.04960.x PUBMED: 16262701 ISSN: 1742-464X EISSN: 1742-4658 FEBS Journal https://hal.univ-brest.fr/hal-00750438 FEBS Journal, 2005, 272 (21), pp.5635-52. ⟨10.1111/j.1742-4658.2005.04960.x⟩ MESH: Animals MESH: Anoxia MESH: Gene Expression Regulation MESH: HSP70 Heat-Shock Proteins MESH: Metallothionein MESH: Ostreidae MESH: Pacific Ocean [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] info:eu-repo/semantics/article Journal articles 2005 ftinsu https://doi.org/10.1111/j.1742-4658.2005.04960.x 2024-01-24T17:23:25Z International audience The molecular response to hypoxia stress in aquatic invertebrates remains relatively unknown. In this study, we investigated the response of the Pacific oyster Crassostrea gigas to hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific genes associated with hypoxia response. A suppression subtractive hybridization method was used to identify specific hypoxia up- and downregulated genes, in gills, mantle and digestive gland, after 7-10 days and 24 days of exposure. This method revealed 616 different sequences corresponding to 12 major physiological functions. The expression of eight potentially regulated genes was analysed by RT-PCR in different tissues at different sampling times over the time course of hypoxia. These genes are implicated in different physiological pathways such as respiration (carbonic anhydrase), carbohydrate metabolism (glycogen phosphorylase), lipid metabolism (delta-9 desaturase), oxidative metabolism and the immune system (glutathione peroxidase), protein regulation (BTF3, transcription factor), nucleic acid regulation (myc homologue), metal sequestration (putative metallothionein) and stress response (heat shock protein 70). Stress proteins (metallothioneins and heat shock proteins) were also quantified. This study contributes to the characterization of many potential genetic markers that could be used in future environmental monitoring, and could lead to explore new mechanisms of stress tolerance in marine mollusc species. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific FEBS Journal 272 21 5635 5652 |