In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas
International audience We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ...
Published in: | Marine Biotechnology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hal.univ-brest.fr/hal-00724496 https://doi.org/10.1007/s10126-011-9421-4 |
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ftinsu:oai:HAL:hal-00724496v1 2024-02-11T10:03:02+01:00 In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas Huvet, Arnaud Fleury, Elodie Corporeau, Charlotte Quillien, Virgile Daniel, Jean-Yves Rivière, Guillaume Boudry, Pierre Fabioux, Caroline Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL) Unité Littoral (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2012 https://hal.univ-brest.fr/hal-00724496 https://doi.org/10.1007/s10126-011-9421-4 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-011-9421-4 hal-00724496 https://hal.univ-brest.fr/hal-00724496 doi:10.1007/s10126-011-9421-4 ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://hal.univ-brest.fr/hal-00724496 Marine Biotechnology, 2012, 14 (4), pp.402-410. ⟨10.1007/s10126-011-9421-4⟩ Marine bivalve Crassostrea gigas Reproduction RNA interference Transforming growth factor-beta [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftinsu https://doi.org/10.1007/s10126-011-9421-4 2024-01-24T17:23:31Z International audience We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated into the gonad, likely as part of the active resorption of degenerating germ cells. Conversely, males showed a normal phenotype at the cellular level, with spermatids and spermatozoids observed in the gonads of control and injected males. As a result, og-TGF beta appears to play an essential role in C. gigas germ cell development by functioning as an activator of germ cell proliferation in both male and female oysters and vitellogenesis in females. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Marine Biotechnology 14 4 402 410 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Marine bivalve Crassostrea gigas Reproduction RNA interference Transforming growth factor-beta [SDE]Environmental Sciences |
spellingShingle |
Marine bivalve Crassostrea gigas Reproduction RNA interference Transforming growth factor-beta [SDE]Environmental Sciences Huvet, Arnaud Fleury, Elodie Corporeau, Charlotte Quillien, Virgile Daniel, Jean-Yves Rivière, Guillaume Boudry, Pierre Fabioux, Caroline In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
topic_facet |
Marine bivalve Crassostrea gigas Reproduction RNA interference Transforming growth factor-beta [SDE]Environmental Sciences |
description |
International audience We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated into the gonad, likely as part of the active resorption of degenerating germ cells. Conversely, males showed a normal phenotype at the cellular level, with spermatids and spermatozoids observed in the gonads of control and injected males. As a result, og-TGF beta appears to play an essential role in C. gigas germ cell development by functioning as an activator of germ cell proliferation in both male and female oysters and vitellogenesis in females. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL) Unité Littoral (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Huvet, Arnaud Fleury, Elodie Corporeau, Charlotte Quillien, Virgile Daniel, Jean-Yves Rivière, Guillaume Boudry, Pierre Fabioux, Caroline |
author_facet |
Huvet, Arnaud Fleury, Elodie Corporeau, Charlotte Quillien, Virgile Daniel, Jean-Yves Rivière, Guillaume Boudry, Pierre Fabioux, Caroline |
author_sort |
Huvet, Arnaud |
title |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_short |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_full |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_fullStr |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_sort |
in vivo rna interference of a gonad-specific transforming growth factor-beta in the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00724496 https://doi.org/10.1007/s10126-011-9421-4 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://hal.univ-brest.fr/hal-00724496 Marine Biotechnology, 2012, 14 (4), pp.402-410. ⟨10.1007/s10126-011-9421-4⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-011-9421-4 hal-00724496 https://hal.univ-brest.fr/hal-00724496 doi:10.1007/s10126-011-9421-4 |
op_doi |
https://doi.org/10.1007/s10126-011-9421-4 |
container_title |
Marine Biotechnology |
container_volume |
14 |
container_issue |
4 |
container_start_page |
402 |
op_container_end_page |
410 |
_version_ |
1790599175804551168 |