The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas
International audience In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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ftinsu:oai:HAL:hal-00670286v1 2024-02-11T10:03:07+01:00 The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas Séguineau, Catherine Racotta, Llie, S. Palacios, Elena Delaporte, Maryse Moal, Jeanne Soudant, Philippe Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Programa de Acuicultura CIBNOR Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2011 https://hal.univ-brest.fr/hal-00670286 https://doi.org/10.1016/j.cbpa.2011.05.011 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2011.05.011 hal-00670286 https://hal.univ-brest.fr/hal-00670286 doi:10.1016/j.cbpa.2011.05.011 ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal.univ-brest.fr/hal-00670286 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 2011, 160 (1), pp.87-93. ⟨10.1016/j.cbpa.2011.05.011⟩ Prostaglandins n-6 unsaturated fatty acids Lipid peroxidation Antioxidants ROS production [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2011 ftinsu https://doi.org/10.1016/j.cbpa.2011.05.011 2024-01-24T17:23:45Z International audience In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation of these cellular responses resulted from changes of ARA-related prostaglandin (PG) production, we analysed prostaglandin E2 metabolite (PGEM) content on the same oysters fed three levels of ARA. Dietary supply of polyunsaturated fatty acids (PUFA) could also induce an oxidative stress that could similarly increase cellular responses; therefore, two indicators of oxidative stress were analysed: peroxidation level and antioxidant defence status. Together the observed positive correlation between ARA and PGEM levels and the absence of lipid peroxidation and antioxidant activity changes supports the hypothesis of an immune stimulation via PG synthesis. Although ARA proportion in oyster tissues increased by up to 7-fold in response to ARA dietary supplementation, peroxidation index did not change because of a compensatory decrease in n-3 fatty acid proportion, mainly 22:6n-3. To further confirm the involvement of PG in the changes of haemocyte count, phagocytosis and ROS production upon ARA supplementation, it would be interesting to test cyclooxygenase and lipooxygenase inhibitors in similar experiments. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Pacific Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 160 1 87 93 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Prostaglandins n-6 unsaturated fatty acids Lipid peroxidation Antioxidants ROS production [SDE]Environmental Sciences |
spellingShingle |
Prostaglandins n-6 unsaturated fatty acids Lipid peroxidation Antioxidants ROS production [SDE]Environmental Sciences Séguineau, Catherine Racotta, Llie, S. Palacios, Elena Delaporte, Maryse Moal, Jeanne Soudant, Philippe The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
topic_facet |
Prostaglandins n-6 unsaturated fatty acids Lipid peroxidation Antioxidants ROS production [SDE]Environmental Sciences |
description |
International audience In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation of these cellular responses resulted from changes of ARA-related prostaglandin (PG) production, we analysed prostaglandin E2 metabolite (PGEM) content on the same oysters fed three levels of ARA. Dietary supply of polyunsaturated fatty acids (PUFA) could also induce an oxidative stress that could similarly increase cellular responses; therefore, two indicators of oxidative stress were analysed: peroxidation level and antioxidant defence status. Together the observed positive correlation between ARA and PGEM levels and the absence of lipid peroxidation and antioxidant activity changes supports the hypothesis of an immune stimulation via PG synthesis. Although ARA proportion in oyster tissues increased by up to 7-fold in response to ARA dietary supplementation, peroxidation index did not change because of a compensatory decrease in n-3 fatty acid proportion, mainly 22:6n-3. To further confirm the involvement of PG in the changes of haemocyte count, phagocytosis and ROS production upon ARA supplementation, it would be interesting to test cyclooxygenase and lipooxygenase inhibitors in similar experiments. |
author2 |
Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Programa de Acuicultura CIBNOR Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Séguineau, Catherine Racotta, Llie, S. Palacios, Elena Delaporte, Maryse Moal, Jeanne Soudant, Philippe |
author_facet |
Séguineau, Catherine Racotta, Llie, S. Palacios, Elena Delaporte, Maryse Moal, Jeanne Soudant, Philippe |
author_sort |
Séguineau, Catherine |
title |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_short |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_full |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_fullStr |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas |
title_sort |
influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.univ-brest.fr/hal-00670286 https://doi.org/10.1016/j.cbpa.2011.05.011 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://hal.univ-brest.fr/hal-00670286 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 2011, 160 (1), pp.87-93. ⟨10.1016/j.cbpa.2011.05.011⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2011.05.011 hal-00670286 https://hal.univ-brest.fr/hal-00670286 doi:10.1016/j.cbpa.2011.05.011 |
op_doi |
https://doi.org/10.1016/j.cbpa.2011.05.011 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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160 |
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