Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions
International audience Within the framework of a national scientific program named "MORtalités ESTivales de l'huître creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassost...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2007
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Subjects: | |
Online Access: | https://hal.univ-brest.fr/hal-00667069 https://doi.org/10.1016/j.jembe.2007.09.003 |
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ftinsu:oai:HAL:hal-00667069v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Crassostrea gigas Genetic selection Hemocyte parameters Reactive oxygen species (ROS) Reproduction Summer mortality [SDE]Environmental Sciences |
spellingShingle |
Crassostrea gigas Genetic selection Hemocyte parameters Reactive oxygen species (ROS) Reproduction Summer mortality [SDE]Environmental Sciences Delaporte, Maryse Soudant, Philippe Lambert, Christophe Jegaden, Marine Moal, Jeanne Pouvreau, Stéphane Degremont, Lionel Boudry, Pierre Samain, Jean-François Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions |
topic_facet |
Crassostrea gigas Genetic selection Hemocyte parameters Reactive oxygen species (ROS) Reproduction Summer mortality [SDE]Environmental Sciences |
description |
International audience Within the framework of a national scientific program named "MORtalités ESTivales de l'huître creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassostrea gigas. As part of the MOREST project, the second generation of three resistant families and two susceptible families were chosen and pooled into two respective groups: "R" and "S". These two groups of oysters were conditioned for 6 months on two food levels (4% and 12% of oyster soft-tissue dry weight in algal dry weight per day) with a temperature gradient that mimicked the Marennes-Oléron natural cycle during the oyster reproductive period. Oyster mortality remained low for the first two months, but then rapidly increased in July when seawater temperature reached 19 °C and above. Mortality was higher in "S" oysters than in "R" oysters, and also higher in oysters fed the 12% diet than those fed 4%, resulting in a decreasing, relative order in cumulative mortality as follows; 12% "S" > 12% "R" > 4% "S" > 4% "R". Although the observed mortality rates were lower than those previously observed in the field, the mortality differential between "R" and "S" oysters was similar. Gonadal development, estimated by tissue lipid content, followed a relative order yielding a direct, positive relationship between reproductive effort and mortality as we reported precedently by quantitative histology. Regarding hemocyte parameters, one of the most striking observations was that reactive oxygen species (ROS) production was significantly higher in "S" oysters than in "R" oysters in May and June, regardless of food level. The absence of known environmental stress under these experimental conditions suggests that the ROS increase in "S" oyster could be related to their higher reproductive activity. Finally, a higher increase in hyalinocyte counts was observed for"S" oysters, compared to "R" oysters, in July, just before mortality. Taken ... |
author2 |
Laboratoire de Physiologie des Invertébrés (LPI) Physiologie Fonctionnelle des Organismes Marins (PFOM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie (LGP) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) |
format |
Article in Journal/Newspaper |
author |
Delaporte, Maryse Soudant, Philippe Lambert, Christophe Jegaden, Marine Moal, Jeanne Pouvreau, Stéphane Degremont, Lionel Boudry, Pierre Samain, Jean-François |
author_facet |
Delaporte, Maryse Soudant, Philippe Lambert, Christophe Jegaden, Marine Moal, Jeanne Pouvreau, Stéphane Degremont, Lionel Boudry, Pierre Samain, Jean-François |
author_sort |
Delaporte, Maryse |
title |
Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions |
title_short |
Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions |
title_full |
Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions |
title_fullStr |
Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions |
title_full_unstemmed |
Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions |
title_sort |
characterisation of physiological and immunological differences between pacific oysters (crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.univ-brest.fr/hal-00667069 https://doi.org/10.1016/j.jembe.2007.09.003 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.univ-brest.fr/hal-00667069 Journal of Experimental Marine Biology and Ecology, 2007, 353 (1), pp.45-57. ⟨10.1016/j.jembe.2007.09.003⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2007.09.003 hal-00667069 https://hal.univ-brest.fr/hal-00667069 doi:10.1016/j.jembe.2007.09.003 |
op_doi |
https://doi.org/10.1016/j.jembe.2007.09.003 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
353 |
container_issue |
1 |
container_start_page |
45 |
op_container_end_page |
57 |
_version_ |
1796941023399641088 |
spelling |
ftinsu:oai:HAL:hal-00667069v1 2024-04-21T08:00:34+00:00 Characterisation of physiological and immunological differences between Pacific oysters (Crassostrea gigas) genetically selected for high or low survival to summer mortalities and fed different rations under controlled conditions Delaporte, Maryse Soudant, Philippe Lambert, Christophe Jegaden, Marine Moal, Jeanne Pouvreau, Stéphane Degremont, Lionel Boudry, Pierre Samain, Jean-François Laboratoire de Physiologie des Invertébrés (LPI) Physiologie Fonctionnelle des Organismes Marins (PFOM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie (LGP) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) 2007 https://hal.univ-brest.fr/hal-00667069 https://doi.org/10.1016/j.jembe.2007.09.003 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2007.09.003 hal-00667069 https://hal.univ-brest.fr/hal-00667069 doi:10.1016/j.jembe.2007.09.003 ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.univ-brest.fr/hal-00667069 Journal of Experimental Marine Biology and Ecology, 2007, 353 (1), pp.45-57. ⟨10.1016/j.jembe.2007.09.003⟩ Crassostrea gigas Genetic selection Hemocyte parameters Reactive oxygen species (ROS) Reproduction Summer mortality [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2007 ftinsu https://doi.org/10.1016/j.jembe.2007.09.003 2024-04-05T00:24:13Z International audience Within the framework of a national scientific program named "MORtalités ESTivales de l'huître creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassostrea gigas. As part of the MOREST project, the second generation of three resistant families and two susceptible families were chosen and pooled into two respective groups: "R" and "S". These two groups of oysters were conditioned for 6 months on two food levels (4% and 12% of oyster soft-tissue dry weight in algal dry weight per day) with a temperature gradient that mimicked the Marennes-Oléron natural cycle during the oyster reproductive period. Oyster mortality remained low for the first two months, but then rapidly increased in July when seawater temperature reached 19 °C and above. Mortality was higher in "S" oysters than in "R" oysters, and also higher in oysters fed the 12% diet than those fed 4%, resulting in a decreasing, relative order in cumulative mortality as follows; 12% "S" > 12% "R" > 4% "S" > 4% "R". Although the observed mortality rates were lower than those previously observed in the field, the mortality differential between "R" and "S" oysters was similar. Gonadal development, estimated by tissue lipid content, followed a relative order yielding a direct, positive relationship between reproductive effort and mortality as we reported precedently by quantitative histology. Regarding hemocyte parameters, one of the most striking observations was that reactive oxygen species (ROS) production was significantly higher in "S" oysters than in "R" oysters in May and June, regardless of food level. The absence of known environmental stress under these experimental conditions suggests that the ROS increase in "S" oyster could be related to their higher reproductive activity. Finally, a higher increase in hyalinocyte counts was observed for"S" oysters, compared to "R" oysters, in July, just before mortality. Taken ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Journal of Experimental Marine Biology and Ecology 353 1 45 57 |