Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality
International audience Large-scale mortality events have been observed in Pacific oyster Crassostrea gigas on the west coast of France since the early 1980s, particularly during summer. In order to understand the causes of this mortality, two generations of oysters from single-pair matings were stud...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2007
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Subjects: | |
Online Access: | https://hal.univ-brest.fr/hal-00667057 https://doi.org/10.1016/j.jembe.2007.05.016 |
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openpolar |
institution |
Open Polar |
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Institut national des sciences de l'Univers: HAL-INSU |
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ftinsu |
language |
English |
topic |
Crassostrea gigas Delta-9 desaturase Glutamine synthetase Mortality SSCP [SDE]Environmental Sciences |
spellingShingle |
Crassostrea gigas Delta-9 desaturase Glutamine synthetase Mortality SSCP [SDE]Environmental Sciences David, Elise Boudry, Pierre Degremont, Lionel Tanguy, Arnaud Quéré, Nolwenn Samain, Jean-François Moraga, Dario Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality |
topic_facet |
Crassostrea gigas Delta-9 desaturase Glutamine synthetase Mortality SSCP [SDE]Environmental Sciences |
description |
International audience Large-scale mortality events have been observed in Pacific oyster Crassostrea gigas on the west coast of France since the early 1980s, particularly during summer. In order to understand the causes of this mortality, two generations of oysters from single-pair matings were studied in three sites on the French Atlantic coast (Baie-des-Veys, Auray and Ronce-les-Bains). The present paper examines the role of two candidate genes in the susceptibility of oysters to summer mortality, and the selective pressure exerted by such mortality on their polymorphism. Glutamine synthetase (amino-acid metabolism) and delta-9 desaturase (lipid metabolism) genes were studied in the successive generations, using polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). Observed and expected genotypic frequencies were compared. Three different alleles were detected for the glutamine synthetase gene and two for delta-9 desaturase. Allele C of glutamine synthetase seemed to be counter-selected in some second generation families. Allele B of delta-9 desaturase gene was potentially counter-selected at Auray in the families showing higher mortality, and strong selection against BB homozygotes was observed. These observations led us to conclude that any selective effect of summer mortality on allele C of glutamine synthetase gene or allele B of delta-9 desaturase gene could be mediated either directly or via linkage to other loci involved in physiological pathways affecting susceptibility. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie (LGP) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Aquaculture genetics and Breeding Technology Center Virginia Institute of Marine Science (VIMS) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
David, Elise Boudry, Pierre Degremont, Lionel Tanguy, Arnaud Quéré, Nolwenn Samain, Jean-François Moraga, Dario |
author_facet |
David, Elise Boudry, Pierre Degremont, Lionel Tanguy, Arnaud Quéré, Nolwenn Samain, Jean-François Moraga, Dario |
author_sort |
David, Elise |
title |
Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality |
title_short |
Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality |
title_full |
Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality |
title_fullStr |
Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality |
title_full_unstemmed |
Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality |
title_sort |
genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of pacific oyster crassostrea gigas and susceptibility to summer mortality |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.univ-brest.fr/hal-00667057 https://doi.org/10.1016/j.jembe.2007.05.016 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.univ-brest.fr/hal-00667057 Journal of Experimental Marine Biology and Ecology, 2007, 349 (2), pp.272-283. ⟨10.1016/j.jembe.2007.05.016⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2007.05.016 hal-00667057 https://hal.univ-brest.fr/hal-00667057 doi:10.1016/j.jembe.2007.05.016 |
op_doi |
https://doi.org/10.1016/j.jembe.2007.05.016 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
349 |
container_issue |
2 |
container_start_page |
272 |
op_container_end_page |
283 |
_version_ |
1796941021824679936 |
spelling |
ftinsu:oai:HAL:hal-00667057v1 2024-04-21T08:00:33+00:00 Genetic polymorphism of glutamine synthetase and delta-9 desaturase in families of Pacific oyster Crassostrea gigas and susceptibility to summer mortality David, Elise Boudry, Pierre Degremont, Lionel Tanguy, Arnaud Quéré, Nolwenn Samain, Jean-François Moraga, Dario Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie (LGP) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Aquaculture genetics and Breeding Technology Center Virginia Institute of Marine Science (VIMS) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2007 https://hal.univ-brest.fr/hal-00667057 https://doi.org/10.1016/j.jembe.2007.05.016 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2007.05.016 hal-00667057 https://hal.univ-brest.fr/hal-00667057 doi:10.1016/j.jembe.2007.05.016 ISSN: 0022-0981 Journal of Experimental Marine Biology and Ecology https://hal.univ-brest.fr/hal-00667057 Journal of Experimental Marine Biology and Ecology, 2007, 349 (2), pp.272-283. ⟨10.1016/j.jembe.2007.05.016⟩ Crassostrea gigas Delta-9 desaturase Glutamine synthetase Mortality SSCP [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2007 ftinsu https://doi.org/10.1016/j.jembe.2007.05.016 2024-04-05T00:24:13Z International audience Large-scale mortality events have been observed in Pacific oyster Crassostrea gigas on the west coast of France since the early 1980s, particularly during summer. In order to understand the causes of this mortality, two generations of oysters from single-pair matings were studied in three sites on the French Atlantic coast (Baie-des-Veys, Auray and Ronce-les-Bains). The present paper examines the role of two candidate genes in the susceptibility of oysters to summer mortality, and the selective pressure exerted by such mortality on their polymorphism. Glutamine synthetase (amino-acid metabolism) and delta-9 desaturase (lipid metabolism) genes were studied in the successive generations, using polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). Observed and expected genotypic frequencies were compared. Three different alleles were detected for the glutamine synthetase gene and two for delta-9 desaturase. Allele C of glutamine synthetase seemed to be counter-selected in some second generation families. Allele B of delta-9 desaturase gene was potentially counter-selected at Auray in the families showing higher mortality, and strong selection against BB homozygotes was observed. These observations led us to conclude that any selective effect of summer mortality on allele C of glutamine synthetase gene or allele B of delta-9 desaturase gene could be mediated either directly or via linkage to other loci involved in physiological pathways affecting susceptibility. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Journal of Experimental Marine Biology and Ecology 349 2 272 283 |