Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas
International audience Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isol...
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ftinsu:oai:HAL:hal-00663786v1 2024-04-21T08:00:34+00:00 Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas Tanguy, Arnaud Boutet, Isabelle Boudry, Pierre Degremont, Lionel Laroche, Jean Moraga, Dario Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie (LGP) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2006 https://hal.univ-brest.fr/hal-00663786 https://doi.org/10.1016/j.gene.2006.06.005 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2006.06.005 hal-00663786 https://hal.univ-brest.fr/hal-00663786 doi:10.1016/j.gene.2006.06.005 ISSN: 0378-1119 EISSN: 1879-0038 Gene https://hal.univ-brest.fr/hal-00663786 Gene, 2006, 382 (1 Novembre 2006), pp.20-27. ⟨10.1016/j.gene.2006.06.005⟩ PGM PhosphoGlucoMutase UTR unTranslated Region ADI Atrazine-Diuron-Isoprouron [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2006 ftinsu https://doi.org/10.1016/j.gene.2006.06.005 2024-04-05T00:24:13Z International audience Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA expression in oyster tissues in response to xenobiotics (i.e hydrocarbons and pesticides). The results obtained showed that PGM mRNA expression is mostly up-regulated in the first steps of the response to pollutant exposure and is xenobiotic-dependant. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institut national des sciences de l'Univers: HAL-INSU Gene 382 20 27 |
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Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
PGM PhosphoGlucoMutase UTR unTranslated Region ADI Atrazine-Diuron-Isoprouron [SDE]Environmental Sciences |
spellingShingle |
PGM PhosphoGlucoMutase UTR unTranslated Region ADI Atrazine-Diuron-Isoprouron [SDE]Environmental Sciences Tanguy, Arnaud Boutet, Isabelle Boudry, Pierre Degremont, Lionel Laroche, Jean Moraga, Dario Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas |
topic_facet |
PGM PhosphoGlucoMutase UTR unTranslated Region ADI Atrazine-Diuron-Isoprouron [SDE]Environmental Sciences |
description |
International audience Phosphoglucomutase is a key enzyme in glycolysis and has been widely studied in vertebrates and some invertebrates but no molecular information is available in marine invertebrates despite the importance of this marker in ecological and genetical studies. In this work, we isolated a cDNA and the corresponding genomic sequence that encode PGM-2 locus in the Pacific oyster Crassostrea gigas. We used sequences drawn from the database to construct an evolutionary framework for examining the position of mollusc PGM sequences among prokaryotic and eukaryotic homologues and showed that oyster PGM gene organization was closer to vertebrates PGM genes than other invertebrates as previously found in other Lophotrochozoa species. We also investigated PGM mRNA expression in oyster tissues in response to xenobiotics (i.e hydrocarbons and pesticides). The results obtained showed that PGM mRNA expression is mostly up-regulated in the first steps of the response to pollutant exposure and is xenobiotic-dependant. |
author2 |
Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Génétique et Pathologie (LGP) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Tanguy, Arnaud Boutet, Isabelle Boudry, Pierre Degremont, Lionel Laroche, Jean Moraga, Dario |
author_facet |
Tanguy, Arnaud Boutet, Isabelle Boudry, Pierre Degremont, Lionel Laroche, Jean Moraga, Dario |
author_sort |
Tanguy, Arnaud |
title |
Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas |
title_short |
Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas |
title_full |
Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas |
title_fullStr |
Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Molecular identification and expression of the phosphoglucomutase (PGM) gene from the Pacific oyster Crassostrea gigas |
title_sort |
molecular identification and expression of the phosphoglucomutase (pgm) gene from the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2006 |
url |
https://hal.univ-brest.fr/hal-00663786 https://doi.org/10.1016/j.gene.2006.06.005 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0378-1119 EISSN: 1879-0038 Gene https://hal.univ-brest.fr/hal-00663786 Gene, 2006, 382 (1 Novembre 2006), pp.20-27. ⟨10.1016/j.gene.2006.06.005⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2006.06.005 hal-00663786 https://hal.univ-brest.fr/hal-00663786 doi:10.1016/j.gene.2006.06.005 |
op_doi |
https://doi.org/10.1016/j.gene.2006.06.005 |
container_title |
Gene |
container_volume |
382 |
container_start_page |
20 |
op_container_end_page |
27 |
_version_ |
1796941035821072384 |