In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs
International audience Harmful algal blooms (HABs) can have both lethal and sublethal impacts on shellfish. To understand the possible roles of haemocytes in bivalve immune responses to HABs and how the algae are affected by these cells (haemocytes), in vitro tests between cultured harmful algal spe...
Published in: | Cell Biology and Toxicology |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2011
|
Subjects: | |
Online Access: | https://hal.univ-brest.fr/hal-00635820 https://doi.org/10.1007/s10565-011-9186-6 |
id |
ftinsu:oai:HAL:hal-00635820v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Bivalve Haemocyte Harmful algae HAB Immune response Phagocytosis [SDE]Environmental Sciences |
spellingShingle |
Bivalve Haemocyte Harmful algae HAB Immune response Phagocytosis [SDE]Environmental Sciences Hegaret, Helene da Silva, Patricia Mirella Wikfors, Gary, H. Haberkorn, Hansy Shumway, Sandra, E. Soudant, Philippe In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs |
topic_facet |
Bivalve Haemocyte Harmful algae HAB Immune response Phagocytosis [SDE]Environmental Sciences |
description |
International audience Harmful algal blooms (HABs) can have both lethal and sublethal impacts on shellfish. To understand the possible roles of haemocytes in bivalve immune responses to HABs and how the algae are affected by these cells (haemocytes), in vitro tests between cultured harmful algal species and haemocytes of the northern quahog (= hard clam) Mercenaria mercenaria, the soft-shell clam Mya arenaria, the eastern and Pacific oysters Crassostrea virginica and Crassostrea gigas and the Manila clam Ruditapes philippinarum were carried out. Within their respective ranges of distribution, these shellfish species can experience blooms of several HAB species, including Prorocentrum minimum, Heterosigma akashiwo, Alexandrium fundyense, Alexandrium minutum and Karenia spp.; thus, these algal species were chosen for testing. Possible differences in haemocyte variables attributable to harmful algae and also effects of haemolymph and haemocytes on the algae themselves were measured. Using microscopic and flow cytometric observations, changes were measured in haemocytes, including cell morphology, mortality, phagocytosis, adhesion and reactive oxygen species (ROS) production, as well as changes in the physiology and the characteristics of the algal cells, including mortality, size, internal complexity and chlorophyll fluorescence. These experiments suggest different effects of the several species of harmful algae upon bivalve haemocytes. Some harmful algae act as immunostimulants, whereas others are immunosuppressive. P. minimum appears to activate haemocytes, but the other harmful algal species tested seem to cause a suppression of immune functions, generally consisting of decreases in phagocytosis, production of ROS and cell adhesion and besides cause an increase in the percentage of dead haemocytes, which could be attributable to the action of chemical toxins. Microalgal cells exposed to shellfish haemolymph generally showed evidence of algal degradation, e.g. loss of chlorophyll fluorescence and modification ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Department of Marine Sciences University of Connecticut (UCONN) Milford Laboratory Northeast Fisheries Science Center (NEFSC) NOAA National Marine Fisheries Service (NMFS) National Oceanic and Atmospheric Administration (NOAA)-National Oceanic and Atmospheric Administration (NOAA)-NOAA National Marine Fisheries Service (NMFS) National Oceanic and Atmospheric Administration (NOAA)-National Oceanic and Atmospheric Administration (NOAA) |
format |
Article in Journal/Newspaper |
author |
Hegaret, Helene da Silva, Patricia Mirella Wikfors, Gary, H. Haberkorn, Hansy Shumway, Sandra, E. Soudant, Philippe |
author_facet |
Hegaret, Helene da Silva, Patricia Mirella Wikfors, Gary, H. Haberkorn, Hansy Shumway, Sandra, E. Soudant, Philippe |
author_sort |
Hegaret, Helene |
title |
In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs |
title_short |
In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs |
title_full |
In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs |
title_fullStr |
In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs |
title_full_unstemmed |
In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs |
title_sort |
in vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.univ-brest.fr/hal-00635820 https://doi.org/10.1007/s10565-011-9186-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0742-2091 EISSN: 1573-6822 Cell Biology and Toxicology https://hal.univ-brest.fr/hal-00635820 Cell Biology and Toxicology, 2011, 27 (4), pp.249-266. ⟨10.1007/s10565-011-9186-6⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10565-011-9186-6 hal-00635820 https://hal.univ-brest.fr/hal-00635820 doi:10.1007/s10565-011-9186-6 |
op_doi |
https://doi.org/10.1007/s10565-011-9186-6 |
container_title |
Cell Biology and Toxicology |
container_volume |
27 |
container_issue |
4 |
container_start_page |
249 |
op_container_end_page |
266 |
_version_ |
1790599427371565056 |
spelling |
ftinsu:oai:HAL:hal-00635820v1 2024-02-11T10:03:14+01:00 In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs Hegaret, Helene da Silva, Patricia Mirella Wikfors, Gary, H. Haberkorn, Hansy Shumway, Sandra, E. Soudant, Philippe Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Department of Marine Sciences University of Connecticut (UCONN) Milford Laboratory Northeast Fisheries Science Center (NEFSC) NOAA National Marine Fisheries Service (NMFS) National Oceanic and Atmospheric Administration (NOAA)-National Oceanic and Atmospheric Administration (NOAA)-NOAA National Marine Fisheries Service (NMFS) National Oceanic and Atmospheric Administration (NOAA)-National Oceanic and Atmospheric Administration (NOAA) 2011-02-22 https://hal.univ-brest.fr/hal-00635820 https://doi.org/10.1007/s10565-011-9186-6 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10565-011-9186-6 hal-00635820 https://hal.univ-brest.fr/hal-00635820 doi:10.1007/s10565-011-9186-6 ISSN: 0742-2091 EISSN: 1573-6822 Cell Biology and Toxicology https://hal.univ-brest.fr/hal-00635820 Cell Biology and Toxicology, 2011, 27 (4), pp.249-266. ⟨10.1007/s10565-011-9186-6⟩ Bivalve Haemocyte Harmful algae HAB Immune response Phagocytosis [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2011 ftinsu https://doi.org/10.1007/s10565-011-9186-6 2024-01-24T17:23:56Z International audience Harmful algal blooms (HABs) can have both lethal and sublethal impacts on shellfish. To understand the possible roles of haemocytes in bivalve immune responses to HABs and how the algae are affected by these cells (haemocytes), in vitro tests between cultured harmful algal species and haemocytes of the northern quahog (= hard clam) Mercenaria mercenaria, the soft-shell clam Mya arenaria, the eastern and Pacific oysters Crassostrea virginica and Crassostrea gigas and the Manila clam Ruditapes philippinarum were carried out. Within their respective ranges of distribution, these shellfish species can experience blooms of several HAB species, including Prorocentrum minimum, Heterosigma akashiwo, Alexandrium fundyense, Alexandrium minutum and Karenia spp.; thus, these algal species were chosen for testing. Possible differences in haemocyte variables attributable to harmful algae and also effects of haemolymph and haemocytes on the algae themselves were measured. Using microscopic and flow cytometric observations, changes were measured in haemocytes, including cell morphology, mortality, phagocytosis, adhesion and reactive oxygen species (ROS) production, as well as changes in the physiology and the characteristics of the algal cells, including mortality, size, internal complexity and chlorophyll fluorescence. These experiments suggest different effects of the several species of harmful algae upon bivalve haemocytes. Some harmful algae act as immunostimulants, whereas others are immunosuppressive. P. minimum appears to activate haemocytes, but the other harmful algal species tested seem to cause a suppression of immune functions, generally consisting of decreases in phagocytosis, production of ROS and cell adhesion and besides cause an increase in the percentage of dead haemocytes, which could be attributable to the action of chemical toxins. Microalgal cells exposed to shellfish haemolymph generally showed evidence of algal degradation, e.g. loss of chlorophyll fluorescence and modification ... Article in Journal/Newspaper Crassostrea gigas Institut national des sciences de l'Univers: HAL-INSU Pacific Cell Biology and Toxicology 27 4 249 266 |