In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes
International audience Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reac...
Main Authors: | , , , , , , |
---|---|
Other Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2007
|
Subjects: | |
Online Access: | https://hal.science/hal-00616832 https://hal.science/hal-00616832/document https://hal.science/hal-00616832/file/Lambert_et_al_2007_Aquaculture_270_413-421_-auteurs.pdf |
id |
ftinsu:oai:HAL:hal-00616832v1 |
---|---|
record_format |
openpolar |
spelling |
ftinsu:oai:HAL:hal-00616832v1 2024-02-11T10:03:05+01:00 In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes Lambert, Christophe Soudant, Philippe Jegaden, Marine Delaporte, Maryse Labreuche, Yannick Moal, Jeanne Samain, Jean-François Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2007 https://hal.science/hal-00616832 https://hal.science/hal-00616832/document https://hal.science/hal-00616832/file/Lambert_et_al_2007_Aquaculture_270_413-421_-auteurs.pdf en eng HAL CCSD Elsevier hal-00616832 https://hal.science/hal-00616832 https://hal.science/hal-00616832/document https://hal.science/hal-00616832/file/Lambert_et_al_2007_Aquaculture_270_413-421_-auteurs.pdf info:eu-repo/semantics/OpenAccess ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-00616832 Aquaculture, 2007, 270, pp.413-421 Crassostrea gigas Hemocytes Reactive oxygen intermediate (ROI) Reactive nitrogen intermediate (RNI) Flow cytometry NADPHoxidase NO-synthase [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/article Journal articles 2007 ftinsu 2024-01-24T17:24:00Z International audience Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types used NADPH-oxidase- and NO-synthase-like pathways to produce these molecules; the NO-synthase pathway seemed relatively more dominant in hyalinocytes and NADPH-oxidase appeared more effective in granulocytes. These results provide new insights for interpreting the modulation of ROI/RNI production by untreated hemocytes shown by other studies, relative to environmental conditions or physiological status of the oysters. Article in Journal/Newspaper Crassostrea gigas Institut national des sciences de l'Univers: HAL-INSU |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Crassostrea gigas Hemocytes Reactive oxygen intermediate (ROI) Reactive nitrogen intermediate (RNI) Flow cytometry NADPHoxidase NO-synthase [SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
Crassostrea gigas Hemocytes Reactive oxygen intermediate (ROI) Reactive nitrogen intermediate (RNI) Flow cytometry NADPHoxidase NO-synthase [SDV.BA]Life Sciences [q-bio]/Animal biology Lambert, Christophe Soudant, Philippe Jegaden, Marine Delaporte, Maryse Labreuche, Yannick Moal, Jeanne Samain, Jean-François In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
topic_facet |
Crassostrea gigas Hemocytes Reactive oxygen intermediate (ROI) Reactive nitrogen intermediate (RNI) Flow cytometry NADPHoxidase NO-synthase [SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
International audience Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types used NADPH-oxidase- and NO-synthase-like pathways to produce these molecules; the NO-synthase pathway seemed relatively more dominant in hyalinocytes and NADPH-oxidase appeared more effective in granulocytes. These results provide new insights for interpreting the modulation of ROI/RNI production by untreated hemocytes shown by other studies, relative to environmental conditions or physiological status of the oysters. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Lambert, Christophe Soudant, Philippe Jegaden, Marine Delaporte, Maryse Labreuche, Yannick Moal, Jeanne Samain, Jean-François |
author_facet |
Lambert, Christophe Soudant, Philippe Jegaden, Marine Delaporte, Maryse Labreuche, Yannick Moal, Jeanne Samain, Jean-François |
author_sort |
Lambert, Christophe |
title |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_short |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_full |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_fullStr |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_full_unstemmed |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_sort |
in vitro modulation of reactive oxygen and nitrogen intermediate (roi/rni) production in crassostrea gigas hemocytes |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.science/hal-00616832 https://hal.science/hal-00616832/document https://hal.science/hal-00616832/file/Lambert_et_al_2007_Aquaculture_270_413-421_-auteurs.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0044-8486 EISSN: 1873-5622 Aquaculture https://hal.science/hal-00616832 Aquaculture, 2007, 270, pp.413-421 |
op_relation |
hal-00616832 https://hal.science/hal-00616832 https://hal.science/hal-00616832/document https://hal.science/hal-00616832/file/Lambert_et_al_2007_Aquaculture_270_413-421_-auteurs.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1790599232574455808 |