New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish
International audience The diversity of O-polysaccharides (O-antigens) among 28 Aeromonas salmonicida strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new str...
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ftinsu:oai:HAL:anses-04693995v1 2024-09-30T14:45:30+00:00 New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish Ucieklak, Karolina Wojtys-Tekiel, Sylwia Leroy, Garance Le Devendec, Laëtitia Baron, Sandrine Kaszowska, Marta Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences (PAN) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Ploufragan-Plouzané-Niort ANSES Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Mycoplasmologie, Bactériologie et Antibiorésistance (MBA) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) This study was financed through the «ANTIBIOFISH» research project supported by both French ministries in charge of environment and agriculture. This project is part of the national effort to reduce antimicrobial resistance in veterinary medicine called “EcoAntibio2017”. This work was supported by the Polish National Agency for Academic Exchange (NAWA), mobility grant number PPN/BFR/2020/1/00031 and 46907VD-PHC Polonium 2021. This work was supported by the statutory funds of the Laboratory of Microbial Immunochemistry and Vaccines of the Hirszfeld Institute of Immunology & Experimental Therapy, Polish Academy of Sciences. 2024-08-01 https://anses.hal.science/anses-04693995 https://doi.org/10.3390/microorganisms12081575 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms12081575 info:eu-repo/semantics/altIdentifier/pmid/39203417 anses-04693995 https://anses.hal.science/anses-04693995 doi:10.3390/microorganisms12081575 PUBMED: 39203417 PUBMEDCENTRAL: PMC11355949 http://creativecommons.org/licenses/by/ ISSN: 2076-2607 Microorganisms https://anses.hal.science/anses-04693995 Microorganisms, 2024, 12 (8), pp.1575. ⟨10.3390/microorganisms12081575⟩ https://www.mdpi.com/2076-2607/12/8/1575 Aeromonas salmonicida O-polysaccharide diversity HR MAS NMR lipopolysaccharide [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2024 ftinsu https://doi.org/10.3390/microorganisms12081575 2024-09-19T00:31:58Z International audience The diversity of O-polysaccharides (O-antigens) among 28 Aeromonas salmonicida strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by 1H and 13C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The following structure of the linear hexasaccharide repeating unit of A. salmonicida O-antigen has been established: →3)-α-L-Rhap-(1→3)-α-D-ManpNAc-(1→2)-β-D-Glcp-(1→3)-α-L-Rhap2OAc4OAc-(1→3)-β-D-ManpNAc-(1→3)-α-D-Glcp-(1→. This new A. salmonicida O-polysaccharide was detected among two isolates collected from trout and turbot fish in 2010 and 2011, respectively. Further investigations should be conducted to evaluate the distribution of this new O-polysaccharide among a larger collection of isolates, depending on their geographic origin, the species of fish, and the health status of the fish. Article in Journal/Newspaper Turbot Institut national des sciences de l'Univers: HAL-INSU Microorganisms 12 8 1575 |
institution |
Open Polar |
collection |
Institut national des sciences de l'Univers: HAL-INSU |
op_collection_id |
ftinsu |
language |
English |
topic |
Aeromonas salmonicida O-polysaccharide diversity HR MAS NMR lipopolysaccharide [SDV]Life Sciences [q-bio] |
spellingShingle |
Aeromonas salmonicida O-polysaccharide diversity HR MAS NMR lipopolysaccharide [SDV]Life Sciences [q-bio] Ucieklak, Karolina Wojtys-Tekiel, Sylwia Leroy, Garance Le Devendec, Laëtitia Baron, Sandrine Kaszowska, Marta New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish |
topic_facet |
Aeromonas salmonicida O-polysaccharide diversity HR MAS NMR lipopolysaccharide [SDV]Life Sciences [q-bio] |
description |
International audience The diversity of O-polysaccharides (O-antigens) among 28 Aeromonas salmonicida strains isolated from ill fish has been determined by using high-resolution magic angle spinning (HR MAS) NMR spectroscopy. The new O-polysaccharide has been identified in two isolates. This new structure was investigated by 1H and 13C NMR spectroscopy and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The following structure of the linear hexasaccharide repeating unit of A. salmonicida O-antigen has been established: →3)-α-L-Rhap-(1→3)-α-D-ManpNAc-(1→2)-β-D-Glcp-(1→3)-α-L-Rhap2OAc4OAc-(1→3)-β-D-ManpNAc-(1→3)-α-D-Glcp-(1→. This new A. salmonicida O-polysaccharide was detected among two isolates collected from trout and turbot fish in 2010 and 2011, respectively. Further investigations should be conducted to evaluate the distribution of this new O-polysaccharide among a larger collection of isolates, depending on their geographic origin, the species of fish, and the health status of the fish. |
author2 |
Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences (PAN) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Ploufragan-Plouzané-Niort ANSES Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Mycoplasmologie, Bactériologie et Antibiorésistance (MBA) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) This study was financed through the «ANTIBIOFISH» research project supported by both French ministries in charge of environment and agriculture. This project is part of the national effort to reduce antimicrobial resistance in veterinary medicine called “EcoAntibio2017”. This work was supported by the Polish National Agency for Academic Exchange (NAWA), mobility grant number PPN/BFR/2020/1/00031 and 46907VD-PHC Polonium 2021. This work was supported by the statutory funds of the Laboratory of Microbial Immunochemistry and Vaccines of the Hirszfeld Institute of Immunology & Experimental Therapy, Polish Academy of Sciences. |
format |
Article in Journal/Newspaper |
author |
Ucieklak, Karolina Wojtys-Tekiel, Sylwia Leroy, Garance Le Devendec, Laëtitia Baron, Sandrine Kaszowska, Marta |
author_facet |
Ucieklak, Karolina Wojtys-Tekiel, Sylwia Leroy, Garance Le Devendec, Laëtitia Baron, Sandrine Kaszowska, Marta |
author_sort |
Ucieklak, Karolina |
title |
New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish |
title_short |
New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish |
title_full |
New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish |
title_fullStr |
New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish |
title_full_unstemmed |
New Structure of Aeromonas salmonicida O-Polysaccharide Isolated from Ill Farmed Fish |
title_sort |
new structure of aeromonas salmonicida o-polysaccharide isolated from ill farmed fish |
publisher |
HAL CCSD |
publishDate |
2024 |
url |
https://anses.hal.science/anses-04693995 https://doi.org/10.3390/microorganisms12081575 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
ISSN: 2076-2607 Microorganisms https://anses.hal.science/anses-04693995 Microorganisms, 2024, 12 (8), pp.1575. ⟨10.3390/microorganisms12081575⟩ https://www.mdpi.com/2076-2607/12/8/1575 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms12081575 info:eu-repo/semantics/altIdentifier/pmid/39203417 anses-04693995 https://anses.hal.science/anses-04693995 doi:10.3390/microorganisms12081575 PUBMED: 39203417 PUBMEDCENTRAL: PMC11355949 |
op_rights |
http://creativecommons.org/licenses/by/ |
op_doi |
https://doi.org/10.3390/microorganisms12081575 |
container_title |
Microorganisms |
container_volume |
12 |
container_issue |
8 |
container_start_page |
1575 |
_version_ |
1811646120692023296 |