Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia
Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infe...
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Basel . MDPI
2024
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Online Access: | https://reponivs.nivs.rs/handle/123456789/946 https://doi.org/10.3390/ani14091255 http://reponivs.nivs.rs/bitstream/id/2561/animals-14-01255.pdf |
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ftinstvmserbia:oai:reponivs.nivs.rs:123456789/946 2024-06-09T07:50:01+00:00 Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia Šiljegović, Sara Mouillaud, Théo Jiolle, Davy Petrić, Dušan Ignjatović-Ćupina, Aleksandra Vasić, Ana Paupy, Christophe Kavran, Mihaela 2024 https://reponivs.nivs.rs/handle/123456789/946 https://doi.org/10.3390/ani14091255 http://reponivs.nivs.rs/bitstream/id/2561/animals-14-01255.pdf en eng Basel . MDPI info:eu-repo/grantAgreement/MESTD/inst-2020/200117/RS// COST Action CA17108 Secretariat for Urbanism and Environment Protection of Vojvodina Province (Serbia) Loznica Municipality 2076-2615 https://reponivs.nivs.rs/handle/123456789/946 doi:10.3390/ani14091255 http://reponivs.nivs.rs/bitstream/id/2561/animals-14-01255.pdf openAccess https://creativecommons.org/licenses/by/4.0/ BY Animals Dirofilaria sp Setaria tundra mosquito surveillance Cox1 gene article publishedVersion 2024 ftinstvmserbia https://doi.org/10.3390/ani14091255 2024-05-16T14:01:59Z Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to infect deer, has not yet been detected in Serbia but has been documented in neighboring countries. Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes of species found to be infected, in order to identify the potential source of parasite infection. A total of 2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene, 650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin (August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided insights into the preferences of mosquitoes that were positive ... Article in Journal/Newspaper Tundra REPONIVS - Repository of Scientific Institute for Veterinary Medicine of Serbia Animals 14 9 1255 |
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Open Polar |
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REPONIVS - Repository of Scientific Institute for Veterinary Medicine of Serbia |
op_collection_id |
ftinstvmserbia |
language |
English |
topic |
Dirofilaria sp Setaria tundra mosquito surveillance Cox1 gene |
spellingShingle |
Dirofilaria sp Setaria tundra mosquito surveillance Cox1 gene Šiljegović, Sara Mouillaud, Théo Jiolle, Davy Petrić, Dušan Ignjatović-Ćupina, Aleksandra Vasić, Ana Paupy, Christophe Kavran, Mihaela Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia |
topic_facet |
Dirofilaria sp Setaria tundra mosquito surveillance Cox1 gene |
description |
Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to infect deer, has not yet been detected in Serbia but has been documented in neighboring countries. Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes of species found to be infected, in order to identify the potential source of parasite infection. A total of 2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene, 650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin (August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided insights into the preferences of mosquitoes that were positive ... |
format |
Article in Journal/Newspaper |
author |
Šiljegović, Sara Mouillaud, Théo Jiolle, Davy Petrić, Dušan Ignjatović-Ćupina, Aleksandra Vasić, Ana Paupy, Christophe Kavran, Mihaela |
author_facet |
Šiljegović, Sara Mouillaud, Théo Jiolle, Davy Petrić, Dušan Ignjatović-Ćupina, Aleksandra Vasić, Ana Paupy, Christophe Kavran, Mihaela |
author_sort |
Šiljegović, Sara |
title |
Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia |
title_short |
Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia |
title_full |
Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia |
title_fullStr |
Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia |
title_full_unstemmed |
Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Maˇcva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia |
title_sort |
dirofilaria sp. and blood meal analysis in mosquitoes collected in vojvodina and maˇcva, and the first report of setaria tundra (issaitshikoff & rajewskaya, 1928) in serbia |
publisher |
Basel . MDPI |
publishDate |
2024 |
url |
https://reponivs.nivs.rs/handle/123456789/946 https://doi.org/10.3390/ani14091255 http://reponivs.nivs.rs/bitstream/id/2561/animals-14-01255.pdf |
genre |
Tundra |
genre_facet |
Tundra |
op_source |
Animals |
op_relation |
info:eu-repo/grantAgreement/MESTD/inst-2020/200117/RS// COST Action CA17108 Secretariat for Urbanism and Environment Protection of Vojvodina Province (Serbia) Loznica Municipality 2076-2615 https://reponivs.nivs.rs/handle/123456789/946 doi:10.3390/ani14091255 http://reponivs.nivs.rs/bitstream/id/2561/animals-14-01255.pdf |
op_rights |
openAccess https://creativecommons.org/licenses/by/4.0/ BY |
op_doi |
https://doi.org/10.3390/ani14091255 |
container_title |
Animals |
container_volume |
14 |
container_issue |
9 |
container_start_page |
1255 |
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1801382977240825856 |