Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui)
Cytochrome P450 1A (CYP1A) induction is used widely as a biomarker of exposure to pollutants, such as petroleum hydrocarbons, yet CYP1A inducibility has been characterized in few tropical fish. Using Western blot analysis, catalytic assay, and immunohistochemistry, we evaluated CYP1A induction in an...
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Environmental Science and Technology
2006
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Online Access: | https://repositorio.inpa.gov.br/handle/1/18752 https://doi.org/10.1021/es052437i |
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ftinstnpamazon:oai:repositorio:1/18752 2023-05-15T17:58:35+02:00 Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui) Matsuo, Aline Y.O. Woodin, Bruce R. Reddy, Christoper M. Val, Adalberto Luis Stegeman, John J. 2006 https://repositorio.inpa.gov.br/handle/1/18752 https://doi.org/10.1021/es052437i en eng Environmental Science and Technology Volume 40, Número 8, Pags. 2851-2858 https://repositorio.inpa.gov.br/handle/1/18752 doi:10.1021/es052437i Restrito Bioassay Biomarkers Catalysts Concentration (process) Crude Petroleum Environmental Impact Cytochrome P450 1a Fish Humic Substances Immunohistochemistry River Pollution Aromatic Hydrocarbon Receptor Biological Marker Petroleum Biomarker Crude Oil Cytochrome Humic Substance Pollutant Animals Experiment Animals Tissue Bioavailability Biotransformation Catalysis Colossoma Macropomum Concentration (parameters) Controlled Study Environmental Exposure Enzyme Activity Enzyme Induction Marine Environment Nonhuman Protein Expression Tambaqui Western Blotting Animal Brasil Cytochrome P-450 Cyp1a1 Cytochrome P-450 Cyp2b1 Fishes Gills Liver Microsomes Rivers Amazon River South America Animalsia Colossoma Marcopomum Artigo 2006 ftinstnpamazon https://doi.org/10.1021/es052437i 2020-08-26T12:18:38Z Cytochrome P450 1A (CYP1A) induction is used widely as a biomarker of exposure to pollutants, such as petroleum hydrocarbons, yet CYP1A inducibility has been characterized in few tropical fish. Using Western blot analysis, catalytic assay, and immunohistochemistry, we evaluated CYP1A induction in an Amazonian fish (tambaqui; Colossoma macropomum) acclimated to humic substances (HS) and acutely exposed to crude oil. HS are ubiquitous in Amazonian waters, and they are known to affect the bioavailability of pollutants. CYP1A activity was also measured in fish exposed for 10 days to a range of concentrations of HS from both natural and commercial sources. Crude oil induced CYP1A expression in tambaqui, as expected. Exposure to both HS and crude oil resulted in greater levels of CYP1A expression relative to that in fish exposed to petroleum alone. Interestingly, CYP1A induction was also observed in fish exposed to HS alone. Induction by HS was concentration-dependent, and activity was higher in fish exposed to HS from the commercial source than in fish exposed to the HS from the natural source. The use of CYP1A as a biomarker of exposure to pollutants such as petroleum hydrocarbons in fish living in environments rich in humic substances should be considered with caution given that HS themselves induce CYP1A expression. Our results suggest that there may be as yet unknown CYP1A inducing components (aryl hydrocarbon receptor agonists) in humic substances. © 2006 American Chemical Society. Article in Journal/Newspaper Petroleum Hydrocarbons in Fish Repositório do INPA Environmental Science & Technology 40 8 2851 2858 |
institution |
Open Polar |
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Repositório do INPA |
op_collection_id |
ftinstnpamazon |
language |
English |
topic |
Bioassay Biomarkers Catalysts Concentration (process) Crude Petroleum Environmental Impact Cytochrome P450 1a Fish Humic Substances Immunohistochemistry River Pollution Aromatic Hydrocarbon Receptor Biological Marker Petroleum Biomarker Crude Oil Cytochrome Humic Substance Pollutant Animals Experiment Animals Tissue Bioavailability Biotransformation Catalysis Colossoma Macropomum Concentration (parameters) Controlled Study Environmental Exposure Enzyme Activity Enzyme Induction Marine Environment Nonhuman Protein Expression Tambaqui Western Blotting Animal Brasil Cytochrome P-450 Cyp1a1 Cytochrome P-450 Cyp2b1 Fishes Gills Liver Microsomes Rivers Amazon River South America Animalsia Colossoma Marcopomum |
spellingShingle |
Bioassay Biomarkers Catalysts Concentration (process) Crude Petroleum Environmental Impact Cytochrome P450 1a Fish Humic Substances Immunohistochemistry River Pollution Aromatic Hydrocarbon Receptor Biological Marker Petroleum Biomarker Crude Oil Cytochrome Humic Substance Pollutant Animals Experiment Animals Tissue Bioavailability Biotransformation Catalysis Colossoma Macropomum Concentration (parameters) Controlled Study Environmental Exposure Enzyme Activity Enzyme Induction Marine Environment Nonhuman Protein Expression Tambaqui Western Blotting Animal Brasil Cytochrome P-450 Cyp1a1 Cytochrome P-450 Cyp2b1 Fishes Gills Liver Microsomes Rivers Amazon River South America Animalsia Colossoma Marcopomum Matsuo, Aline Y.O. Woodin, Bruce R. Reddy, Christoper M. Val, Adalberto Luis Stegeman, John J. Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui) |
topic_facet |
Bioassay Biomarkers Catalysts Concentration (process) Crude Petroleum Environmental Impact Cytochrome P450 1a Fish Humic Substances Immunohistochemistry River Pollution Aromatic Hydrocarbon Receptor Biological Marker Petroleum Biomarker Crude Oil Cytochrome Humic Substance Pollutant Animals Experiment Animals Tissue Bioavailability Biotransformation Catalysis Colossoma Macropomum Concentration (parameters) Controlled Study Environmental Exposure Enzyme Activity Enzyme Induction Marine Environment Nonhuman Protein Expression Tambaqui Western Blotting Animal Brasil Cytochrome P-450 Cyp1a1 Cytochrome P-450 Cyp2b1 Fishes Gills Liver Microsomes Rivers Amazon River South America Animalsia Colossoma Marcopomum |
description |
Cytochrome P450 1A (CYP1A) induction is used widely as a biomarker of exposure to pollutants, such as petroleum hydrocarbons, yet CYP1A inducibility has been characterized in few tropical fish. Using Western blot analysis, catalytic assay, and immunohistochemistry, we evaluated CYP1A induction in an Amazonian fish (tambaqui; Colossoma macropomum) acclimated to humic substances (HS) and acutely exposed to crude oil. HS are ubiquitous in Amazonian waters, and they are known to affect the bioavailability of pollutants. CYP1A activity was also measured in fish exposed for 10 days to a range of concentrations of HS from both natural and commercial sources. Crude oil induced CYP1A expression in tambaqui, as expected. Exposure to both HS and crude oil resulted in greater levels of CYP1A expression relative to that in fish exposed to petroleum alone. Interestingly, CYP1A induction was also observed in fish exposed to HS alone. Induction by HS was concentration-dependent, and activity was higher in fish exposed to HS from the commercial source than in fish exposed to the HS from the natural source. The use of CYP1A as a biomarker of exposure to pollutants such as petroleum hydrocarbons in fish living in environments rich in humic substances should be considered with caution given that HS themselves induce CYP1A expression. Our results suggest that there may be as yet unknown CYP1A inducing components (aryl hydrocarbon receptor agonists) in humic substances. © 2006 American Chemical Society. |
format |
Article in Journal/Newspaper |
author |
Matsuo, Aline Y.O. Woodin, Bruce R. Reddy, Christoper M. Val, Adalberto Luis Stegeman, John J. |
author_facet |
Matsuo, Aline Y.O. Woodin, Bruce R. Reddy, Christoper M. Val, Adalberto Luis Stegeman, John J. |
author_sort |
Matsuo, Aline Y.O. |
title |
Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui) |
title_short |
Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui) |
title_full |
Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui) |
title_fullStr |
Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui) |
title_full_unstemmed |
Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui) |
title_sort |
humic substances and crude oil induce cytochrome p450 1a expression in the amazonian fish species colossoma macropomum (tambaqui) |
publisher |
Environmental Science and Technology |
publishDate |
2006 |
url |
https://repositorio.inpa.gov.br/handle/1/18752 https://doi.org/10.1021/es052437i |
genre |
Petroleum Hydrocarbons in Fish |
genre_facet |
Petroleum Hydrocarbons in Fish |
op_relation |
Volume 40, Número 8, Pags. 2851-2858 https://repositorio.inpa.gov.br/handle/1/18752 doi:10.1021/es052437i |
op_rights |
Restrito |
op_doi |
https://doi.org/10.1021/es052437i |
container_title |
Environmental Science & Technology |
container_volume |
40 |
container_issue |
8 |
container_start_page |
2851 |
op_container_end_page |
2858 |
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1766167260126248960 |