Survey of Brucella spp. and Leptospira spp. Antibodies in cetaceans and manatees of the Amazon basin and Atlantic Ocean, Brazil
Aquatic mammals can act as sentinels of emerging and resurging pathogens in the environment. Brucella spp. and Leptospira spp. are 2 zoonotic pathogens relevant to aquatic mammals, and their detection can be used to assess pathogen exposure. In this study, serum from 84 individuals — 63 cetaceans (f...
Published in: | Diseases of Aquatic Organisms |
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Diseases of Aquatic Organisms
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Online Access: | https://repositorio.inpa.gov.br/handle/1/16985 https://doi.org/10.3354/dao03312 |
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ftinstnpamazon:oai:repositorio:1/16985 2023-05-15T17:03:40+02:00 Survey of Brucella spp. and Leptospira spp. Antibodies in cetaceans and manatees of the Amazon basin and Atlantic Ocean, Brazil Sánchez-Sarmiento, Angélica María Carvalho, Vítor Luz Meirelles, Ana Carolina Oliveira de Gravena, Waleska Marigo, Juliana Sacristán, Carlos Costa-Silva, Samira Groch, Kátia Regina dos Santos Silva, Nairléia Neto, Josè Soares Ferreira Catão-Dias, José Luiz 2018 https://repositorio.inpa.gov.br/handle/1/16985 https://doi.org/10.3354/dao03312 en eng Diseases of Aquatic Organisms Volume 132, Número 1, Pags. 1-11 https://repositorio.inpa.gov.br/handle/1/16985 doi:10.3354/dao03312 Restrito Antibody Bacterium Cetacean Epidemiology Infectious Disease Marine Mammal Pathogen Serum Testing Method Amazon Basin Atlantic Ocean Brasil Brucella Cetacea Delphinidae Feresa Feresa Attenuata Globicephala Macrorhynchus Iniidae Kogiidae Leptospira Mammalia Nia Peponocephala Peponocephala Electra Stenella Clymene Trichechidae Trichechus Manatus Tursiops Tursiops Truncatus Bacterium Antibody Animals Brucellosis Questionnaires Trichechus Animal Antibodies Bacterial Surveys And Questionnaires Artigo 2018 ftinstnpamazon https://doi.org/10.3354/dao03312 2020-08-26T12:24:01Z Aquatic mammals can act as sentinels of emerging and resurging pathogens in the environment. Brucella spp. and Leptospira spp. are 2 zoonotic pathogens relevant to aquatic mammals, and their detection can be used to assess pathogen exposure. In this study, serum from 84 individuals — 63 cetaceans (families Iniidae, n = 37; Delphinidae, n = 22; and Kogiidae, n = 4) and 21 West Indian manatees Trichechus manatus — was tested by the Rose Bengal Test (RBT) and a commercial competitive enzyme-linked immunosorbent assay (c-ELISA) for detecting Brucella spp. antibodies, and the microscopic agglutination test (MAT) for screening Leptospira spp. exposure. Overall, 4.8 % (3/63) of cetaceans were positive by RBT and 15.9 % (10/63) by c-ELISA for Brucella spp. Serum from 8 c-ELISA positive cetaceans (with available serum) was further tested via serum agglutination test (SAT) and 1 individual was positive. c-ELISA was more sensitive than RBT. Exposure to Brucella spp. was found in 5 cetacean species: Clymene dolphin Stenella clymene, short-finned pilot whale Globicephala macrorhynchus, pygmy killer whale Feresa attenuata, melon-headed whale Peponocephala electra and Atlantic bottlenose dolphin Tursiops truncatus in the Atlantic Ocean, Brazil, expanding the range of known Brucella seropositive aquatic hosts. No evidence of Brucella spp. exposure was found in Iniidae and Kogiidae odontocetes and manatees. Antibodies against Leptospira spp. were not detected in cetaceans and sire-nians by MAT. These results contribute to the evaluation of different Brucella spp. serological methods in cetaceans and manatees and highlight the epidemiology of zoonotic pathogens in aquatic mammals of the southwestern Atlantic Ocean and the Amazon basin. © Inter-Research 2018. Article in Journal/Newspaper Killer Whale Killer whale Repositório do INPA Indian Diseases of Aquatic Organisms 132 1 1 11 |
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Repositório do INPA |
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ftinstnpamazon |
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English |
topic |
Antibody Bacterium Cetacean Epidemiology Infectious Disease Marine Mammal Pathogen Serum Testing Method Amazon Basin Atlantic Ocean Brasil Brucella Cetacea Delphinidae Feresa Feresa Attenuata Globicephala Macrorhynchus Iniidae Kogiidae Leptospira Mammalia Nia Peponocephala Peponocephala Electra Stenella Clymene Trichechidae Trichechus Manatus Tursiops Tursiops Truncatus Bacterium Antibody Animals Brucellosis Questionnaires Trichechus Animal Antibodies Bacterial Surveys And Questionnaires |
spellingShingle |
Antibody Bacterium Cetacean Epidemiology Infectious Disease Marine Mammal Pathogen Serum Testing Method Amazon Basin Atlantic Ocean Brasil Brucella Cetacea Delphinidae Feresa Feresa Attenuata Globicephala Macrorhynchus Iniidae Kogiidae Leptospira Mammalia Nia Peponocephala Peponocephala Electra Stenella Clymene Trichechidae Trichechus Manatus Tursiops Tursiops Truncatus Bacterium Antibody Animals Brucellosis Questionnaires Trichechus Animal Antibodies Bacterial Surveys And Questionnaires Sánchez-Sarmiento, Angélica María Carvalho, Vítor Luz Meirelles, Ana Carolina Oliveira de Gravena, Waleska Marigo, Juliana Sacristán, Carlos Costa-Silva, Samira Groch, Kátia Regina dos Santos Silva, Nairléia Neto, Josè Soares Ferreira Catão-Dias, José Luiz Survey of Brucella spp. and Leptospira spp. Antibodies in cetaceans and manatees of the Amazon basin and Atlantic Ocean, Brazil |
topic_facet |
Antibody Bacterium Cetacean Epidemiology Infectious Disease Marine Mammal Pathogen Serum Testing Method Amazon Basin Atlantic Ocean Brasil Brucella Cetacea Delphinidae Feresa Feresa Attenuata Globicephala Macrorhynchus Iniidae Kogiidae Leptospira Mammalia Nia Peponocephala Peponocephala Electra Stenella Clymene Trichechidae Trichechus Manatus Tursiops Tursiops Truncatus Bacterium Antibody Animals Brucellosis Questionnaires Trichechus Animal Antibodies Bacterial Surveys And Questionnaires |
description |
Aquatic mammals can act as sentinels of emerging and resurging pathogens in the environment. Brucella spp. and Leptospira spp. are 2 zoonotic pathogens relevant to aquatic mammals, and their detection can be used to assess pathogen exposure. In this study, serum from 84 individuals — 63 cetaceans (families Iniidae, n = 37; Delphinidae, n = 22; and Kogiidae, n = 4) and 21 West Indian manatees Trichechus manatus — was tested by the Rose Bengal Test (RBT) and a commercial competitive enzyme-linked immunosorbent assay (c-ELISA) for detecting Brucella spp. antibodies, and the microscopic agglutination test (MAT) for screening Leptospira spp. exposure. Overall, 4.8 % (3/63) of cetaceans were positive by RBT and 15.9 % (10/63) by c-ELISA for Brucella spp. Serum from 8 c-ELISA positive cetaceans (with available serum) was further tested via serum agglutination test (SAT) and 1 individual was positive. c-ELISA was more sensitive than RBT. Exposure to Brucella spp. was found in 5 cetacean species: Clymene dolphin Stenella clymene, short-finned pilot whale Globicephala macrorhynchus, pygmy killer whale Feresa attenuata, melon-headed whale Peponocephala electra and Atlantic bottlenose dolphin Tursiops truncatus in the Atlantic Ocean, Brazil, expanding the range of known Brucella seropositive aquatic hosts. No evidence of Brucella spp. exposure was found in Iniidae and Kogiidae odontocetes and manatees. Antibodies against Leptospira spp. were not detected in cetaceans and sire-nians by MAT. These results contribute to the evaluation of different Brucella spp. serological methods in cetaceans and manatees and highlight the epidemiology of zoonotic pathogens in aquatic mammals of the southwestern Atlantic Ocean and the Amazon basin. © Inter-Research 2018. |
format |
Article in Journal/Newspaper |
author |
Sánchez-Sarmiento, Angélica María Carvalho, Vítor Luz Meirelles, Ana Carolina Oliveira de Gravena, Waleska Marigo, Juliana Sacristán, Carlos Costa-Silva, Samira Groch, Kátia Regina dos Santos Silva, Nairléia Neto, Josè Soares Ferreira Catão-Dias, José Luiz |
author_facet |
Sánchez-Sarmiento, Angélica María Carvalho, Vítor Luz Meirelles, Ana Carolina Oliveira de Gravena, Waleska Marigo, Juliana Sacristán, Carlos Costa-Silva, Samira Groch, Kátia Regina dos Santos Silva, Nairléia Neto, Josè Soares Ferreira Catão-Dias, José Luiz |
author_sort |
Sánchez-Sarmiento, Angélica María |
title |
Survey of Brucella spp. and Leptospira spp. Antibodies in cetaceans and manatees of the Amazon basin and Atlantic Ocean, Brazil |
title_short |
Survey of Brucella spp. and Leptospira spp. Antibodies in cetaceans and manatees of the Amazon basin and Atlantic Ocean, Brazil |
title_full |
Survey of Brucella spp. and Leptospira spp. Antibodies in cetaceans and manatees of the Amazon basin and Atlantic Ocean, Brazil |
title_fullStr |
Survey of Brucella spp. and Leptospira spp. Antibodies in cetaceans and manatees of the Amazon basin and Atlantic Ocean, Brazil |
title_full_unstemmed |
Survey of Brucella spp. and Leptospira spp. Antibodies in cetaceans and manatees of the Amazon basin and Atlantic Ocean, Brazil |
title_sort |
survey of brucella spp. and leptospira spp. antibodies in cetaceans and manatees of the amazon basin and atlantic ocean, brazil |
publisher |
Diseases of Aquatic Organisms |
publishDate |
2018 |
url |
https://repositorio.inpa.gov.br/handle/1/16985 https://doi.org/10.3354/dao03312 |
geographic |
Indian |
geographic_facet |
Indian |
genre |
Killer Whale Killer whale |
genre_facet |
Killer Whale Killer whale |
op_relation |
Volume 132, Número 1, Pags. 1-11 https://repositorio.inpa.gov.br/handle/1/16985 doi:10.3354/dao03312 |
op_rights |
Restrito |
op_doi |
https://doi.org/10.3354/dao03312 |
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Diseases of Aquatic Organisms |
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132 |
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1 |
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1 |
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11 |
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1766057574835159040 |