In vitro mechanistic differences in benzo[a]pyrene–DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems
Turbot (Scophthalmus maximus) and mussel (Mytilus edulis) microsomes were incubated with DNA to examine if microsomal in vitro metabolism of BaP could result in DNA adducts detected by 32P-postlabelling. Turbot DNA was incubated with benzo[a]pyrene (BaP), NADPH and microsomal activating systems prep...
Published in: | Marine Environmental Research |
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Online Access: | https://repository.icr.ac.uk/handle/internal/2728 https://doi.org/10.1016/S0141-1136(02)00190-3 |
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ftinstcancerres:oai:repository.icr.ac.uk:internal/2728 2023-05-15T18:15:50+02:00 In vitro mechanistic differences in benzo[a]pyrene–DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems Peters, LD Telli-Karakoç, F Hewer, A Phillips, DH Phillips, David Hunter 2018-09-17T15:14:35Z - 503 https://repository.icr.ac.uk/handle/internal/2728 https://doi.org/10.1016/S0141-1136(02)00190-3 eng eng 3 Marine Environmental Research, 2002, 54 pp. 499 - 503 0141-1136 https://repository.icr.ac.uk/handle/internal/2728 doi:10.1016/S0141-1136(02)00190-3 Journal Article 2018 ftinstcancerres https://doi.org/10.1016/S0141-1136(02)00190-3 2022-12-18T08:58:28Z Turbot (Scophthalmus maximus) and mussel (Mytilus edulis) microsomes were incubated with DNA to examine if microsomal in vitro metabolism of BaP could result in DNA adducts detected by 32P-postlabelling. Turbot DNA was incubated with benzo[a]pyrene (BaP), NADPH and microsomal activating systems prepared from either livers of unexposed turbot, turbot exposed to BaP or β-naphthoflavone (ß-NF) or digestive glands from mussels. The β-NF activating system generated the highest levels of DNA adducts detected in this study (451.7 adducts per 108 nucleotides) and were distributed in three discrete adduct TLC spots, one of which (97% of the total adducts) co-migrated with the 32P-postlabelled BaP 7,8-diol, 9,10-epoxide-N2-guanine adduct. Fewer adducts (P <0.05) were generated by BaP-induced microsomes (9.4–30.6 adducts per 108 nucleotides) but levels were higher (P <0.05) than those generated from untreated fish (3.5 adducts per 108 nucleotides). Co-incubation with 500 μM α-naphthoflavone (α-NF) resulted in 97–99% inhibition in adduct formation implicating cytochrome P450-dependent (CYP) bioactivation however there was some evidence for carry over of BaP in the liver microsomal preparations from BaP injected fish. In contrast to the fish activating systems, no DNA adducts were observed when mussel microsomes were incubated with BaP, DNA and NADPH. Article in Journal/Newspaper Scophthalmus maximus Turbot The Institute of Cancer Research (ICR): Publications Repository Marine Environmental Research 54 3-5 499 503 |
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Open Polar |
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The Institute of Cancer Research (ICR): Publications Repository |
op_collection_id |
ftinstcancerres |
language |
English |
description |
Turbot (Scophthalmus maximus) and mussel (Mytilus edulis) microsomes were incubated with DNA to examine if microsomal in vitro metabolism of BaP could result in DNA adducts detected by 32P-postlabelling. Turbot DNA was incubated with benzo[a]pyrene (BaP), NADPH and microsomal activating systems prepared from either livers of unexposed turbot, turbot exposed to BaP or β-naphthoflavone (ß-NF) or digestive glands from mussels. The β-NF activating system generated the highest levels of DNA adducts detected in this study (451.7 adducts per 108 nucleotides) and were distributed in three discrete adduct TLC spots, one of which (97% of the total adducts) co-migrated with the 32P-postlabelled BaP 7,8-diol, 9,10-epoxide-N2-guanine adduct. Fewer adducts (P <0.05) were generated by BaP-induced microsomes (9.4–30.6 adducts per 108 nucleotides) but levels were higher (P <0.05) than those generated from untreated fish (3.5 adducts per 108 nucleotides). Co-incubation with 500 μM α-naphthoflavone (α-NF) resulted in 97–99% inhibition in adduct formation implicating cytochrome P450-dependent (CYP) bioactivation however there was some evidence for carry over of BaP in the liver microsomal preparations from BaP injected fish. In contrast to the fish activating systems, no DNA adducts were observed when mussel microsomes were incubated with BaP, DNA and NADPH. |
author2 |
Phillips, David Hunter |
format |
Article in Journal/Newspaper |
author |
Peters, LD Telli-Karakoç, F Hewer, A Phillips, DH |
spellingShingle |
Peters, LD Telli-Karakoç, F Hewer, A Phillips, DH In vitro mechanistic differences in benzo[a]pyrene–DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems |
author_facet |
Peters, LD Telli-Karakoç, F Hewer, A Phillips, DH |
author_sort |
Peters, LD |
title |
In vitro mechanistic differences in benzo[a]pyrene–DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems |
title_short |
In vitro mechanistic differences in benzo[a]pyrene–DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems |
title_full |
In vitro mechanistic differences in benzo[a]pyrene–DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems |
title_fullStr |
In vitro mechanistic differences in benzo[a]pyrene–DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems |
title_full_unstemmed |
In vitro mechanistic differences in benzo[a]pyrene–DNA adduct formation using fish liver and mussel digestive gland microsomal activating systems |
title_sort |
in vitro mechanistic differences in benzo[a]pyrene–dna adduct formation using fish liver and mussel digestive gland microsomal activating systems |
publishDate |
2018 |
url |
https://repository.icr.ac.uk/handle/internal/2728 https://doi.org/10.1016/S0141-1136(02)00190-3 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
3 Marine Environmental Research, 2002, 54 pp. 499 - 503 0141-1136 https://repository.icr.ac.uk/handle/internal/2728 doi:10.1016/S0141-1136(02)00190-3 |
op_doi |
https://doi.org/10.1016/S0141-1136(02)00190-3 |
container_title |
Marine Environmental Research |
container_volume |
54 |
container_issue |
3-5 |
container_start_page |
499 |
op_container_end_page |
503 |
_version_ |
1766189059541041152 |