First report of recently described cryptic species Hydatigera kamiyai in Serbia

• Main Authors: Miljević, Milan, Rajičić, Marija, Umhang, Gerald, Bjelić Čabrilo, Olivera, Bajić, Branka, Budinski, Ivana, Blagojević, Jelena
• Other Authors: Petrović, Tamaš
• Format: Conference Object
• Language: srp; English
• Published: Belgrade: Veterinary Association of Serbia 2023
• Subjects: Hydatigera kamiyai; rodents; Serbia; parasites; glodari; Srbija; paraziti; Microtus arvalis; Siberia
• Online Access: http://radar.ibiss.bg.ac.rs/handle/123456789/6033; https://radar.ibiss.bg.ac.rs/bitstream/id/14406/BookodAbstracts25thSymposiumofepizootiologistandepidemiologist-pages-2-4,75-76.pdf; https://hdl.handle.net/21.15107/rcub_ibiss_6033

The family Taeniidae recently consists of 4 genera, with a newly described cryptic species H. kamiyai within the Hydatigera genus. H. kamiyai is distributed across Europe to western Siberia, whereas H. taeniaeformis s.s. probably originated in Asia but has spread worldwide. Despite the extraordinary genetic divergence, the two species differ little morphologically, making it questionable whether morphological identification is even relevant in this case. Considering the geographical location of the studied area and the intermediate hosts targeted, we expected the presence of H. kamiyai, and our aim was to confirm this assumption by genetic analyses. From March to October 2013 to 2022, a total of 846 small mammals (Apodemus agrarius (152), Apodemus flavicollis (521), Apodemus sylvaticus (33), Microtus arvalis (48), Microtus subteraneus (17), Myodes glareolus (52), Crocidura suaveolens (13), Crocidura leucodon (7) and Sorex araneus (3)) were captured from 45 different localities in Serbia. Cysts and visible lesions in the body cavity and various organs were examined. Two markers (cox1 and 12S rDNA) were used for amplification and sequencing. A total of 21 strobilocercus of H. kamiyai were identified corresponding to thirteen sequences of the cox1 gene (318 bp) and eight of 12S rDNA (238 bp). H. kamiyai is found in 17 A. flavicollis, one A. agrarius, two M. arvalis, and one C. leucodon. According to the median-joining network, the 13 H. kamiyai cox1 sequences from this study clustered with isolates from Europe and western Siberia differing up to 2.7%. These results confirm and highlight the different geographic distribution and molecular differences in the Hydatigera genus. The results also confirm host suitability for this species. This is the first report of this newly described cryptic species in our country confirmed by genetic analysis. Фамилија Taeniidae од недавно се састоји од 4 рода, са ново-описаном криптичном врстом Hydatigera kamiyai у оквиру рода Hydatigera. H. kamiyai врста је распрострањена широм ...