Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia
Background Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu la...
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Springer Nature
2023
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ftinstbiss:oai:radar.ibiss.bg.ac.rs:123456789/6016 2023-10-25T01:40:33+02:00 Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia Miljević, Milan Rajičić, Marija Umhang, Gerald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena 2023 http://radar.ibiss.bg.ac.rs/handle/123456789/6016 https://doi.org/10.1186/s13071-023-05879-x https://radar.ibiss.bg.ac.rs/bitstream/id/14551/bitstream_14551.pdf en eng Springer Nature info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// 1756-3305 http://radar.ibiss.bg.ac.rs/handle/123456789/6016 doi:10.1186/s13071-023-05879-x 37491284 https://radar.ibiss.bg.ac.rs/bitstream/id/14551/bitstream_14551.pdf openAccess https://creativecommons.org/licenses/by/4.0/ BY © 2023, The Author(s) Parasites and Vectors Parasite Taenia Rodents cox1 12S rDNA Phylogenetics article publishedVersion 2023 ftinstbiss https://doi.org/10.1186/s13071-023-05879-x 2023-09-26T16:28:06Z Background Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu lato (s.l.) species is difficult without the use of molecular methods. The aim of this study was to identify and explore the genetic diversity of Hydatigera and other taeniid species. Methods Ten different small mammals species (856 individuals) (Rattus rattus, three Apodemus, three Arvicolinae and three Soricidae species) were examined from 2013 to 2023. Captured animals were visually examined for cysts and visible lesions. Two markers were used for amplification and sequencing: cox1 and 12S rDNA. Results Molecular analysis of cysts and visible lesions revealed four taeniid species: Hydatigera kamiyai, H. taeniaeformis sensu stricto (s.s.), Taenia martis and T. crassiceps. Hydatigera kamiyai was found in Apodemus flavicollis, A. agrarius, Microtus arvalis and Crocidrua leucodon, while H. taeniaeformis s.s. is registered in R. rattus. Hydatigera kamiyai cox1 sequences clustered with European populations and showed at least 25 nucleotid differences compared to Asian, African, Australian and one of our isolates of H. taeniaeformis s.s acquired from a rat, followed by large sequence distances (9.4% to 12.9%), indicating clear molecular distinction of two species. Conclusions This is one of the few mitochondrial gene-based studies performed after the description of cryptic entities within the Hydatigera taeniaeformis s.l. complex and represents a valuable contribution to understanding of genetic diversity, host suitability and geographic distribution of these tapeworm species. Also, our study provides an important basis of molecular data from this part of Europe for further studies. We emphasize the importance of additional studies of intermediate hosts, especially rats from Europe and Apodemus spp. and voles from Asia and ... Article in Journal/Newspaper Microtus arvalis Rattus rattus RADaR - Digital Repository of Archived Publications Institute for Biological Research "Sinisa Stankovic" Parasites & Vectors 16 1 |
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Open Polar |
collection |
RADaR - Digital Repository of Archived Publications Institute for Biological Research "Sinisa Stankovic" |
op_collection_id |
ftinstbiss |
language |
English |
topic |
Parasite Taenia Rodents cox1 12S rDNA Phylogenetics |
spellingShingle |
Parasite Taenia Rodents cox1 12S rDNA Phylogenetics Miljević, Milan Rajičić, Marija Umhang, Gerald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
topic_facet |
Parasite Taenia Rodents cox1 12S rDNA Phylogenetics |
description |
Background Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu lato (s.l.) species is difficult without the use of molecular methods. The aim of this study was to identify and explore the genetic diversity of Hydatigera and other taeniid species. Methods Ten different small mammals species (856 individuals) (Rattus rattus, three Apodemus, three Arvicolinae and three Soricidae species) were examined from 2013 to 2023. Captured animals were visually examined for cysts and visible lesions. Two markers were used for amplification and sequencing: cox1 and 12S rDNA. Results Molecular analysis of cysts and visible lesions revealed four taeniid species: Hydatigera kamiyai, H. taeniaeformis sensu stricto (s.s.), Taenia martis and T. crassiceps. Hydatigera kamiyai was found in Apodemus flavicollis, A. agrarius, Microtus arvalis and Crocidrua leucodon, while H. taeniaeformis s.s. is registered in R. rattus. Hydatigera kamiyai cox1 sequences clustered with European populations and showed at least 25 nucleotid differences compared to Asian, African, Australian and one of our isolates of H. taeniaeformis s.s acquired from a rat, followed by large sequence distances (9.4% to 12.9%), indicating clear molecular distinction of two species. Conclusions This is one of the few mitochondrial gene-based studies performed after the description of cryptic entities within the Hydatigera taeniaeformis s.l. complex and represents a valuable contribution to understanding of genetic diversity, host suitability and geographic distribution of these tapeworm species. Also, our study provides an important basis of molecular data from this part of Europe for further studies. We emphasize the importance of additional studies of intermediate hosts, especially rats from Europe and Apodemus spp. and voles from Asia and ... |
format |
Article in Journal/Newspaper |
author |
Miljević, Milan Rajičić, Marija Umhang, Gerald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena |
author_facet |
Miljević, Milan Rajičić, Marija Umhang, Gerald Bajić, Branka Bjelić Čabrilo, Olivera Budinski, Ivana Blagojević, Jelena |
author_sort |
Miljević, Milan |
title |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_short |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_full |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_fullStr |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_full_unstemmed |
Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia |
title_sort |
cryptic species hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in serbia |
publisher |
Springer Nature |
publishDate |
2023 |
url |
http://radar.ibiss.bg.ac.rs/handle/123456789/6016 https://doi.org/10.1186/s13071-023-05879-x https://radar.ibiss.bg.ac.rs/bitstream/id/14551/bitstream_14551.pdf |
genre |
Microtus arvalis Rattus rattus |
genre_facet |
Microtus arvalis Rattus rattus |
op_source |
Parasites and Vectors |
op_relation |
info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// 1756-3305 http://radar.ibiss.bg.ac.rs/handle/123456789/6016 doi:10.1186/s13071-023-05879-x 37491284 https://radar.ibiss.bg.ac.rs/bitstream/id/14551/bitstream_14551.pdf |
op_rights |
openAccess https://creativecommons.org/licenses/by/4.0/ BY © 2023, The Author(s) |
op_doi |
https://doi.org/10.1186/s13071-023-05879-x |
container_title |
Parasites & Vectors |
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16 |
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1 |
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