Evidence of recombination in Tula virus strains from Serbia
Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions a...
Published in: | Infection, Genetics and Evolution |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | https://radar.ibiss.bg.ac.rs/handle/123456789/2295 https://doi.org/10.1016/j.meegid.2013.08.020 |
id |
ftinstbiss:oai:radar.ibiss.bg.ac.rs:123456789/2295 |
---|---|
record_format |
openpolar |
spelling |
ftinstbiss:oai:radar.ibiss.bg.ac.rs:123456789/2295 2023-05-15T17:12:34+02:00 Evidence of recombination in Tula virus strains from Serbia Nikolic, Valentina Stajkovic, Novica Stamenković, Gorana Cekanac, Radovan Marusic, Predrag Siljic, Marina Gligic, Ana Stanojevic, Maja 2016-05-23T11:00:47Z https://radar.ibiss.bg.ac.rs/handle/123456789/2295 https://doi.org/10.1016/j.meegid.2013.08.020 English eng 1567-7257 https://radar.ibiss.bg.ac.rs/handle/123456789/2295 doi:10.1016/j.meegid.2013.08.020 2-s2.0-84893774623 000332273800056 restrictedAccess ARR Infection Genetics and Evolution Recombination Tula virus Phylogenetic analysis Molecular evolution article publishedVersion 2016 ftinstbiss https://doi.org/10.1016/j.meegid.2013.08.020 2023-03-08T15:01:23Z Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved. Ministry of Education and Science Republic of Serbia {[}175024] Article in Journal/Newspaper Microtus arvalis RADaR - Digital Repository of Archived Publications Institute for Biological Research "Sinisa Stankovic" Tula ENVELOPE(-65.650,-65.650,-65.517,-65.517) Infection, Genetics and Evolution 21 472 478 |
institution |
Open Polar |
collection |
RADaR - Digital Repository of Archived Publications Institute for Biological Research "Sinisa Stankovic" |
op_collection_id |
ftinstbiss |
language |
English |
topic |
Recombination Tula virus Phylogenetic analysis Molecular evolution |
spellingShingle |
Recombination Tula virus Phylogenetic analysis Molecular evolution Nikolic, Valentina Stajkovic, Novica Stamenković, Gorana Cekanac, Radovan Marusic, Predrag Siljic, Marina Gligic, Ana Stanojevic, Maja Evidence of recombination in Tula virus strains from Serbia |
topic_facet |
Recombination Tula virus Phylogenetic analysis Molecular evolution |
description |
Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved. Ministry of Education and Science Republic of Serbia {[}175024] |
format |
Article in Journal/Newspaper |
author |
Nikolic, Valentina Stajkovic, Novica Stamenković, Gorana Cekanac, Radovan Marusic, Predrag Siljic, Marina Gligic, Ana Stanojevic, Maja |
author_facet |
Nikolic, Valentina Stajkovic, Novica Stamenković, Gorana Cekanac, Radovan Marusic, Predrag Siljic, Marina Gligic, Ana Stanojevic, Maja |
author_sort |
Nikolic, Valentina |
title |
Evidence of recombination in Tula virus strains from Serbia |
title_short |
Evidence of recombination in Tula virus strains from Serbia |
title_full |
Evidence of recombination in Tula virus strains from Serbia |
title_fullStr |
Evidence of recombination in Tula virus strains from Serbia |
title_full_unstemmed |
Evidence of recombination in Tula virus strains from Serbia |
title_sort |
evidence of recombination in tula virus strains from serbia |
publishDate |
2016 |
url |
https://radar.ibiss.bg.ac.rs/handle/123456789/2295 https://doi.org/10.1016/j.meegid.2013.08.020 |
long_lat |
ENVELOPE(-65.650,-65.650,-65.517,-65.517) |
geographic |
Tula |
geographic_facet |
Tula |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Infection Genetics and Evolution |
op_relation |
1567-7257 https://radar.ibiss.bg.ac.rs/handle/123456789/2295 doi:10.1016/j.meegid.2013.08.020 2-s2.0-84893774623 000332273800056 |
op_rights |
restrictedAccess ARR |
op_doi |
https://doi.org/10.1016/j.meegid.2013.08.020 |
container_title |
Infection, Genetics and Evolution |
container_volume |
21 |
container_start_page |
472 |
op_container_end_page |
478 |
_version_ |
1766069361402970112 |