Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles
International audience Notodiscus hookeri is the only native land snail of the sub-Antarctic present in Crozet Archipelago. Two shell ecophenotypes co-exist on Possession Island and feed exclusively on lichens [1] that are symbiotic organisms widely distributed in sub-Antarctic islands. To improve o...
Published in: | Planta Medica |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2016
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Online Access: | https://univ-rennes.hal.science/hal-01446729 https://doi.org/10.1055/s-0036-1596670 |
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ftinsarennhal:oai:HAL:hal-01446729v1 |
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openpolar |
institution |
Open Polar |
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INSA Rennes HAL (Institut National des Sciences Appliquées) |
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ftinsarennhal |
language |
English |
topic |
Sub-antarctic lichens metabolites profiling DART-MS Notodiscus hookeri chemical ecology [CHIM]Chemical Sciences |
spellingShingle |
Sub-antarctic lichens metabolites profiling DART-MS Notodiscus hookeri chemical ecology [CHIM]Chemical Sciences Gadea, Alice Lohézic-Le Dévéhat, Françoise Le Lamer, Anne-Cécile Le Pogam, P. Ertz, Damien Charrier, Maryvonne Boustie, Joël Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
topic_facet |
Sub-antarctic lichens metabolites profiling DART-MS Notodiscus hookeri chemical ecology [CHIM]Chemical Sciences |
description |
International audience Notodiscus hookeri is the only native land snail of the sub-Antarctic present in Crozet Archipelago. Two shell ecophenotypes co-exist on Possession Island and feed exclusively on lichens [1] that are symbiotic organisms widely distributed in sub-Antarctic islands. To improve our understanding of lichen-snail trophic interactions, the endemic tripartite lichen Argopsis friesiana was selected. The snail belonging to the organic ecophenotype occurs sympatrically with A. friesiana. Profiling and quantification of the major secondary metabolites in the whole lichen were carried out by LC-DAD-MS. Two main compounds were identified, the depside atranorin (8.2 mg g-1 DM) and the depsidone argopsin. Besides, a screening of primary metabolites was carried out to determine which compounds could be useful for the snail energetic demands. Glutamate, glutamine, gamma-aminobutyric acid and alpha-alanine were the main amino acids found in the lichen in low concentrations (0.4 mg·g-1 DM). Carbohydrates, including arabitol (10.2 mg g-1), mannitol (4.0.10-4 mg g-1) and sucrose (3.0.10-4 mg g-1), were also identified. In cultivation, N. hookeri snails were fed with fructified thalli of A. friesiana. Snails (n = 20) ate the podetia cortex and phyllocladia but they clearly avoided apothecia, the reproductive organs. This sectorial damage of the lichen by the snail suggested a differential distribution of the metabolites. Therefore, a profiling of secondary compounds was performed by Direct Analysis in Real Time – Mass Spectroscopy (DART-MS) [2] on each morphological part of the lichen (phyllocladia, apothecia, podetia and cephalodia). The identification and distribution of secondary metabolites, fatty acids, amino acids and carbohydrates should help us to determine whether metabolites affect snail feeding choice and which of them could be involved. |
author2 |
Institut des Sciences Chimiques de Rennes (ISCR) Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes, biodiversité, évolution Rennes (ECOBIO) Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) Pharmacochimie et Biologie pour le Développement (PHARMA-DEV) Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT) Meise Botanic Garden Belgium (Plantentuin) |
format |
Article in Journal/Newspaper |
author |
Gadea, Alice Lohézic-Le Dévéhat, Françoise Le Lamer, Anne-Cécile Le Pogam, P. Ertz, Damien Charrier, Maryvonne Boustie, Joël |
author_facet |
Gadea, Alice Lohézic-Le Dévéhat, Françoise Le Lamer, Anne-Cécile Le Pogam, P. Ertz, Damien Charrier, Maryvonne Boustie, Joël |
author_sort |
Gadea, Alice |
title |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_short |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_full |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_fullStr |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_full_unstemmed |
Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles |
title_sort |
sectorial land snail damage to the lichen argopsis friesiana could be explained by metabolite profiles |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://univ-rennes.hal.science/hal-01446729 https://doi.org/10.1055/s-0036-1596670 |
genre |
Antarc* Antarctic Possession Island |
genre_facet |
Antarc* Antarctic Possession Island |
op_source |
ISSN: 0032-0943 EISSN: 1439-0221 Planta Medica https://univ-rennes.hal.science/hal-01446729 Planta Medica, 2016, 81 (S 01), pp.S1-S381. ⟨10.1055/s-0036-1596670⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0036-1596670 info:eu-repo/semantics/altIdentifier/pmid/27975871 hal-01446729 https://univ-rennes.hal.science/hal-01446729 doi:10.1055/s-0036-1596670 PUBMED: 27975871 |
op_doi |
https://doi.org/10.1055/s-0036-1596670 |
container_title |
Planta Medica |
container_volume |
81 |
container_issue |
S 01 |
container_start_page |
S1 |
op_container_end_page |
S381 |
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1810492959292915712 |
spelling |
ftinsarennhal:oai:HAL:hal-01446729v1 2024-09-15T17:45:13+00:00 Sectorial land snail damage to the lichen Argopsis friesiana could be explained by metabolite profiles Gadea, Alice Lohézic-Le Dévéhat, Françoise Le Lamer, Anne-Cécile Le Pogam, P. Ertz, Damien Charrier, Maryvonne Boustie, Joël Institut des Sciences Chimiques de Rennes (ISCR) Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes) Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Ecosystèmes, biodiversité, évolution Rennes (ECOBIO) Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) Pharmacochimie et Biologie pour le Développement (PHARMA-DEV) Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT) Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) Université de Toulouse (UT) Meise Botanic Garden Belgium (Plantentuin) 2016 https://univ-rennes.hal.science/hal-01446729 https://doi.org/10.1055/s-0036-1596670 en eng HAL CCSD Georg Thieme Verlag info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0036-1596670 info:eu-repo/semantics/altIdentifier/pmid/27975871 hal-01446729 https://univ-rennes.hal.science/hal-01446729 doi:10.1055/s-0036-1596670 PUBMED: 27975871 ISSN: 0032-0943 EISSN: 1439-0221 Planta Medica https://univ-rennes.hal.science/hal-01446729 Planta Medica, 2016, 81 (S 01), pp.S1-S381. ⟨10.1055/s-0036-1596670⟩ Sub-antarctic lichens metabolites profiling DART-MS Notodiscus hookeri chemical ecology [CHIM]Chemical Sciences info:eu-repo/semantics/article Journal articles 2016 ftinsarennhal https://doi.org/10.1055/s-0036-1596670 2024-07-24T23:31:36Z International audience Notodiscus hookeri is the only native land snail of the sub-Antarctic present in Crozet Archipelago. Two shell ecophenotypes co-exist on Possession Island and feed exclusively on lichens [1] that are symbiotic organisms widely distributed in sub-Antarctic islands. To improve our understanding of lichen-snail trophic interactions, the endemic tripartite lichen Argopsis friesiana was selected. The snail belonging to the organic ecophenotype occurs sympatrically with A. friesiana. Profiling and quantification of the major secondary metabolites in the whole lichen were carried out by LC-DAD-MS. Two main compounds were identified, the depside atranorin (8.2 mg g-1 DM) and the depsidone argopsin. Besides, a screening of primary metabolites was carried out to determine which compounds could be useful for the snail energetic demands. Glutamate, glutamine, gamma-aminobutyric acid and alpha-alanine were the main amino acids found in the lichen in low concentrations (0.4 mg·g-1 DM). Carbohydrates, including arabitol (10.2 mg g-1), mannitol (4.0.10-4 mg g-1) and sucrose (3.0.10-4 mg g-1), were also identified. In cultivation, N. hookeri snails were fed with fructified thalli of A. friesiana. Snails (n = 20) ate the podetia cortex and phyllocladia but they clearly avoided apothecia, the reproductive organs. This sectorial damage of the lichen by the snail suggested a differential distribution of the metabolites. Therefore, a profiling of secondary compounds was performed by Direct Analysis in Real Time – Mass Spectroscopy (DART-MS) [2] on each morphological part of the lichen (phyllocladia, apothecia, podetia and cephalodia). The identification and distribution of secondary metabolites, fatty acids, amino acids and carbohydrates should help us to determine whether metabolites affect snail feeding choice and which of them could be involved. Article in Journal/Newspaper Antarc* Antarctic Possession Island INSA Rennes HAL (Institut National des Sciences Appliquées) Planta Medica 81 S 01 S1 S381 |