Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow?
International audience The effect of nutrients on microbial interactions, including competition and collaboration, has mainly been studied in laboratories, but their potential application to complex ecosystems is unknown. Here, we examined the effect of changes in organic acids among other parameter...
Published in: | Frontiers in Microbiology |
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Main Authors: | , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2019
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Online Access: | https://hal.science/hal-02415106 https://hal.science/hal-02415106/document https://hal.science/hal-02415106/file/fmicb-10-02492.pdf https://doi.org/10.3389/fmicb.2019.02492 |
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ftinsalyonhal:oai:HAL:hal-02415106v1 |
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Open Polar |
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INSA Lyon HAL (Institut National des Sciences Appliquées) |
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ftinsalyonhal |
language |
English |
topic |
[SDV]Life Sciences [q-bio] [SPI]Engineering Sciences [physics] |
spellingShingle |
[SDV]Life Sciences [q-bio] [SPI]Engineering Sciences [physics] Bergk Pinto, Benoît Maccario, Lorrie Dommergue, Aurélien Vogel, Timothy, M. Larose, Catherine Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
topic_facet |
[SDV]Life Sciences [q-bio] [SPI]Engineering Sciences [physics] |
description |
International audience The effect of nutrients on microbial interactions, including competition and collaboration, has mainly been studied in laboratories, but their potential application to complex ecosystems is unknown. Here, we examined the effect of changes in organic acids among other parameters on snow microbial communities in situ over 2 months. We compared snow bacterial communities from a low organic acid content period to that from a higher organic acid period. We hypothesized that an increase in organic acids would shift the dominant microbial interaction from collaboration to competition. To evaluate microbial interactions, we built taxonomic co-variance networks from OTUs obtained from 16S rRNA gene sequencing. In addition, we tracked marker genes of microbial cooperation (plasmid backbone genes) and competition (antibiotic resistance genes) across both sampling periods in metagenomes and metatranscriptomes. Our results showed a decrease in the average connectivity of the network during late spring compared to the early spring that we interpreted as a decrease of cooperation. This observation was strengthened by the significantly more abundant plasmid backbone genes in the metagenomes from the early spring. The modularity of the network from the late spring was also found to be higher than the one from the early spring, which is another possible indicator of increased competition. Antibiotic resistance genes were significantly more abundant in the late spring metagenomes. In addition, antibiotic resistance genes were also positively correlated to the organic acid concentration of the snow across both seasons. Snow organic acid content might be responsible for this change in bacterial interactions in the Arctic snow community. |
author2 |
Ampère, Département Bioingénierie (BioIng) Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Institut des Géosciences de l’Environnement (IGE) Institut de Recherche pour le Développement (IRD)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 ) |
format |
Article in Journal/Newspaper |
author |
Bergk Pinto, Benoît Maccario, Lorrie Dommergue, Aurélien Vogel, Timothy, M. Larose, Catherine |
author_facet |
Bergk Pinto, Benoît Maccario, Lorrie Dommergue, Aurélien Vogel, Timothy, M. Larose, Catherine |
author_sort |
Bergk Pinto, Benoît |
title |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_short |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_full |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_fullStr |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_full_unstemmed |
Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? |
title_sort |
do organic substrates drive microbial community interactions in arctic snow? |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02415106 https://hal.science/hal-02415106/document https://hal.science/hal-02415106/file/fmicb-10-02492.pdf https://doi.org/10.3389/fmicb.2019.02492 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal.science/hal-02415106 Frontiers in Microbiology, 2019, 10, ⟨10.3389/fmicb.2019.02492⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02492 hal-02415106 https://hal.science/hal-02415106 https://hal.science/hal-02415106/document https://hal.science/hal-02415106/file/fmicb-10-02492.pdf doi:10.3389/fmicb.2019.02492 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3389/fmicb.2019.02492 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1801372759708663808 |
spelling |
ftinsalyonhal:oai:HAL:hal-02415106v1 2024-06-09T07:43:56+00:00 Do Organic Substrates Drive Microbial Community Interactions in Arctic Snow? Bergk Pinto, Benoît Maccario, Lorrie Dommergue, Aurélien Vogel, Timothy, M. Larose, Catherine Ampère, Département Bioingénierie (BioIng) Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Institut des Géosciences de l’Environnement (IGE) Institut de Recherche pour le Développement (IRD)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 ) 2019-10-31 https://hal.science/hal-02415106 https://hal.science/hal-02415106/document https://hal.science/hal-02415106/file/fmicb-10-02492.pdf https://doi.org/10.3389/fmicb.2019.02492 en eng HAL CCSD Frontiers Media info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02492 hal-02415106 https://hal.science/hal-02415106 https://hal.science/hal-02415106/document https://hal.science/hal-02415106/file/fmicb-10-02492.pdf doi:10.3389/fmicb.2019.02492 info:eu-repo/semantics/OpenAccess ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology https://hal.science/hal-02415106 Frontiers in Microbiology, 2019, 10, ⟨10.3389/fmicb.2019.02492⟩ [SDV]Life Sciences [q-bio] [SPI]Engineering Sciences [physics] info:eu-repo/semantics/article Journal articles 2019 ftinsalyonhal https://doi.org/10.3389/fmicb.2019.02492 2024-05-16T14:23:06Z International audience The effect of nutrients on microbial interactions, including competition and collaboration, has mainly been studied in laboratories, but their potential application to complex ecosystems is unknown. Here, we examined the effect of changes in organic acids among other parameters on snow microbial communities in situ over 2 months. We compared snow bacterial communities from a low organic acid content period to that from a higher organic acid period. We hypothesized that an increase in organic acids would shift the dominant microbial interaction from collaboration to competition. To evaluate microbial interactions, we built taxonomic co-variance networks from OTUs obtained from 16S rRNA gene sequencing. In addition, we tracked marker genes of microbial cooperation (plasmid backbone genes) and competition (antibiotic resistance genes) across both sampling periods in metagenomes and metatranscriptomes. Our results showed a decrease in the average connectivity of the network during late spring compared to the early spring that we interpreted as a decrease of cooperation. This observation was strengthened by the significantly more abundant plasmid backbone genes in the metagenomes from the early spring. The modularity of the network from the late spring was also found to be higher than the one from the early spring, which is another possible indicator of increased competition. Antibiotic resistance genes were significantly more abundant in the late spring metagenomes. In addition, antibiotic resistance genes were also positively correlated to the organic acid concentration of the snow across both seasons. Snow organic acid content might be responsible for this change in bacterial interactions in the Arctic snow community. Article in Journal/Newspaper Arctic INSA Lyon HAL (Institut National des Sciences Appliquées) Arctic Frontiers in Microbiology 10 |