Large-Scale Metagenomic-Based Study of Antibiotic Resistance in the Environment
International audience Antibiotic resistance, including multiresistance acquisition and dissemination by pathogens, is a critical healthcare issue threatening our management of infectious diseases [ 1-3 ]. Rapid accumulation of resistance phenotypes implies a reservoir of transferable antibiotic res...
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ftinsalyonhal:oai:HAL:hal-00988678v1 2023-11-12T04:24:34+01:00 Large-Scale Metagenomic-Based Study of Antibiotic Resistance in the Environment Nesme, Joseph Cecillon, Sébastien Delmont, Tom Monier, Jean-Michel Vogel, Timothy, M. Simonet, Pascal Ampère, Département Bioingénierie (BioIng) Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) This work was financed by grants from the French Agency for the Environment and Energy Management (ADEME, project Générique) and from French Agency for Food, Environmental and Occupational Health and Safety (ANSES, project Impactance, convention number EST-2012/2/28). The French Ministry of Higher Education and Research funded J.N. T.O.D. was supported by a doctoral fellowship from the Rhône Alpes Région. 2014-05-08 https://hal.science/hal-00988678 https://doi.org/10.1016/j.cub.2014.03.036 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cub.2014.03.036 hal-00988678 https://hal.science/hal-00988678 doi:10.1016/j.cub.2014.03.036 ISSN: 0960-9822 EISSN: 1879-0445 Current Biology - CB https://hal.science/hal-00988678 Current Biology - CB, 2014, pp.voir DOI. ⟨10.1016/j.cub.2014.03.036⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2014 ftinsalyonhal https://doi.org/10.1016/j.cub.2014.03.036 2023-11-01T17:28:14Z International audience Antibiotic resistance, including multiresistance acquisition and dissemination by pathogens, is a critical healthcare issue threatening our management of infectious diseases [ 1-3 ]. Rapid accumulation of resistance phenotypes implies a reservoir of transferable antibiotic resistance gene determinants (ARGDs) selected in response to inhibition of antibiotic concentrations, as found in hospitals [ 1, 3-5 ]. Antibiotic resistance genes were found in environmental isolates, soil DNA [ 4-6 ], secluded caves [ 6, 7 ], and permafrost DNA [ 7, 8 ]. Antibiotics target essential and ubiquitous cell functions, and resistance is a common characteristic of environmental bacteria [ 8-11 ]. Environmental ARGDs are an abundant reservoir of potentially transferable resistance for pathogens [ 9-12 ]. Using metagenomic sequences, we show that ARGDs can be detected in all (n = 71) environments analyzed. Soil metagenomes had the most diverse pool of ARGDs. The most common types of resistances found in environmental metagenomes were efflux pumps and genes conferring resistance to vancomycin, tetracycline, or β-lactam antibiotics used in veterinary and human healthcare. Our study describes the diverse and abundant antibiotic resistance genes in nonclinical environments and shows that these genes are not randomly distributed among different environments (e.g., soil, oceans or human feces). Article in Journal/Newspaper permafrost INSA Lyon HAL (Institut National des Sciences Appliquées) Current Biology 24 10 1096 1100 |
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INSA Lyon HAL (Institut National des Sciences Appliquées) |
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[SDE]Environmental Sciences |
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[SDE]Environmental Sciences Nesme, Joseph Cecillon, Sébastien Delmont, Tom Monier, Jean-Michel Vogel, Timothy, M. Simonet, Pascal Large-Scale Metagenomic-Based Study of Antibiotic Resistance in the Environment |
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[SDE]Environmental Sciences |
description |
International audience Antibiotic resistance, including multiresistance acquisition and dissemination by pathogens, is a critical healthcare issue threatening our management of infectious diseases [ 1-3 ]. Rapid accumulation of resistance phenotypes implies a reservoir of transferable antibiotic resistance gene determinants (ARGDs) selected in response to inhibition of antibiotic concentrations, as found in hospitals [ 1, 3-5 ]. Antibiotic resistance genes were found in environmental isolates, soil DNA [ 4-6 ], secluded caves [ 6, 7 ], and permafrost DNA [ 7, 8 ]. Antibiotics target essential and ubiquitous cell functions, and resistance is a common characteristic of environmental bacteria [ 8-11 ]. Environmental ARGDs are an abundant reservoir of potentially transferable resistance for pathogens [ 9-12 ]. Using metagenomic sequences, we show that ARGDs can be detected in all (n = 71) environments analyzed. Soil metagenomes had the most diverse pool of ARGDs. The most common types of resistances found in environmental metagenomes were efflux pumps and genes conferring resistance to vancomycin, tetracycline, or β-lactam antibiotics used in veterinary and human healthcare. Our study describes the diverse and abundant antibiotic resistance genes in nonclinical environments and shows that these genes are not randomly distributed among different environments (e.g., soil, oceans or human feces). |
author2 |
Ampère, Département Bioingénierie (BioIng) Ampère (AMPERE) École Centrale de Lyon (ECL) Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL) Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) This work was financed by grants from the French Agency for the Environment and Energy Management (ADEME, project Générique) and from French Agency for Food, Environmental and Occupational Health and Safety (ANSES, project Impactance, convention number EST-2012/2/28). The French Ministry of Higher Education and Research funded J.N. T.O.D. was supported by a doctoral fellowship from the Rhône Alpes Région. |
format |
Article in Journal/Newspaper |
author |
Nesme, Joseph Cecillon, Sébastien Delmont, Tom Monier, Jean-Michel Vogel, Timothy, M. Simonet, Pascal |
author_facet |
Nesme, Joseph Cecillon, Sébastien Delmont, Tom Monier, Jean-Michel Vogel, Timothy, M. Simonet, Pascal |
author_sort |
Nesme, Joseph |
title |
Large-Scale Metagenomic-Based Study of Antibiotic Resistance in the Environment |
title_short |
Large-Scale Metagenomic-Based Study of Antibiotic Resistance in the Environment |
title_full |
Large-Scale Metagenomic-Based Study of Antibiotic Resistance in the Environment |
title_fullStr |
Large-Scale Metagenomic-Based Study of Antibiotic Resistance in the Environment |
title_full_unstemmed |
Large-Scale Metagenomic-Based Study of Antibiotic Resistance in the Environment |
title_sort |
large-scale metagenomic-based study of antibiotic resistance in the environment |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.science/hal-00988678 https://doi.org/10.1016/j.cub.2014.03.036 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
ISSN: 0960-9822 EISSN: 1879-0445 Current Biology - CB https://hal.science/hal-00988678 Current Biology - CB, 2014, pp.voir DOI. ⟨10.1016/j.cub.2014.03.036⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cub.2014.03.036 hal-00988678 https://hal.science/hal-00988678 doi:10.1016/j.cub.2014.03.036 |
op_doi |
https://doi.org/10.1016/j.cub.2014.03.036 |
container_title |
Current Biology |
container_volume |
24 |
container_issue |
10 |
container_start_page |
1096 |
op_container_end_page |
1100 |
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1782339057284546560 |