Prostaglandin E2 enhancement of interferon-gamma production by antigen-stimulated type 1 helper T cells.

• Published in: Cellular Immunology
• Main Authors: Bloom, D., Jabrane-Ferrat, N., Zeng, L., Wu, A., Li, L., Lo, D., Turck, C. W., An, S., Goetzl, E. J.
• Other Authors: Department of Medicine, Microbiology and Immunology (UCSF-HHMI), University of California San Francisco (UC San Francisco), University of California (UC)-University of California (UC)-Rosalind Russell Medical Research Center, Center for Research in Ceramic and Composite Materials (CICECO), Universidade de Aveiro, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Forest Ecology and Global Changes, School of Life Sciences
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 1999
• Subjects: MESH: Animals; MESH: Antigens; MESH: Interferon-gamma; MESH: Mice; MESH: Misoprostol; MESH: Myoglobin; MESH: Receptors; Prostaglandin E; EP1 Subtype; EP2 Subtype; EP3 Subtype; EP4 Subtype; MESH: Th1 Cells; MESH: Dinoprostone; MESH: Gene Expression; MESH: Hemagglutinin Glycoproteins; Influenza Virus; [SDV.CAN]Life Sciences [q-bio]/Cancer; Sperm whale
• Online Access: https://hal.science/hal-00641043; https://doi.org/10.1006/cimm.1999.1479

International audience Prostaglandin E2 (PGE2) is a potent mediator generated in immune tissues by cyclooxygenation of arachidonic acid. PGE2 affects T cell functions through four homologous G protein-coupled receptors termed EP1R, EP2R, EP3R, and EP4R that differ in tissue distribution and signaling. Antigen-evoked secretion of interferon-gamma (IFN-gamma) by sperm whale myoglobin-specific Th1 cells of DBA/2 mouse I-Ed-restricted clones, that express EP3Rs and EP4Rs, was enhanced a maximum of 3-fold by 10(-10) to 10(-8) M PGE2 and 2.5-fold each for the EP1R/EP3R-directed agonist sulprostone (10(-8) and 10(-7) M) and for the EP4R/EP3R/EP2R agonist misoprostol (10(-9) M). Neither PGE2 nor the synthetic analogs affected secretion of IFN-gamma by PMA plus ionomycin-stimulated clones of Th1 cells. Antigen-evoked secretion of IFN-gamma by influenza hemagglutinin-specific mouse lymph node Th1 cells, that also express EP3Rs and EP4Rs, was increased a maximum of 12-fold by 10(-9) to 10(-8) M PGE2, 14-fold by 10(-9) M sulprostone, and 10-fold by 10(-9) M misoprostol. Production of IFN-gamma by either type of Th1 cell was not affected significantly by 10(-6) M PGE2 alone. The generation of IFN-gamma by antigen-stimulated Th1 cells thus is significantly enhanced by physiologically relevant concentrations of PGE2.