Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus).
Yelloweye rockfish (Sebastes ruberrimus) is an extremely long-lived species (up to ∼120 years) of fish, which inhabits the coastal waters of Alaska. Due to their long lifespans, yelloweye are known to accumulate high levels of mercury, and potentially other trace elements, in their tissues. Relative...
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Online Access: | https://espace.inrs.ca/id/eprint/7653/ https://espace.inrs.ca/id/eprint/7653/1/P3364.pdf https://doi.org/10.1016/j.envpol.2018.06.077 |
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ftinrsquebec:oai:espace.inrs.ca:7653 2023-05-15T18:03:32+02:00 Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). Barst, Benjamin D. Rosabal, Maikel Drevnick, Paul E. Campbell, Peter G. C. Basu, Niladri 2018 application/pdf https://espace.inrs.ca/id/eprint/7653/ https://espace.inrs.ca/id/eprint/7653/1/P3364.pdf https://doi.org/10.1016/j.envpol.2018.06.077 en eng https://espace.inrs.ca/id/eprint/7653/1/P3364.pdf Barst, Benjamin D., Rosabal, Maikel, Drevnick, Paul E., Campbell, Peter G. C. et Basu, Niladri (2018). Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). Environmental Pollution , vol. 242 . p. 63-72. DOI:10.1016/j.envpol.2018.06.077 <https://doi.org/10.1016/j.envpol.2018.06.077>. doi:10.1016/j.envpol.2018.06.077 cc_by_nc_nd_4 CC-BY-NC-ND subcellular partitioning trace elements mercury detoxification yelloweye rockfish Alaska Article Évalué par les pairs 2018 ftinrsquebec https://doi.org/10.1016/j.envpol.2018.06.077 2023-02-10T11:44:42Z Yelloweye rockfish (Sebastes ruberrimus) is an extremely long-lived species (up to ∼120 years) of fish, which inhabits the coastal waters of Alaska. Due to their long lifespans, yelloweye are known to accumulate high levels of mercury, and potentially other trace elements, in their tissues. Relatively little is known about the subcellular distribution of trace elements in the tissues of yelloweye rockfish; such information can provide important insights into detoxification/toxicity mechanisms at the subcellular level. To address this, we collected yelloweye rockfish (n = 8) from the eastern coast of Prince of Wales Island, Alaska in 2014. We determined the subcellular partitioning of trace elements (cadmium (Cd), lead (Pb), arsenic (As), total mercury (Hg), and selenium (Se)) in yelloweye livers with a partitioning procedure designed to separate liver cells into putative metal-sensitive fractions (cytosolic enzymes, organelles) and detoxified metal fractions (metallothionein or metallothionein-like proteins and peptides, granule-like structures) using differential centrifugation, NaOH digestion, and heat denaturation steps. The resulting fractions were then analyzed for total Hg with a direct Hg analyzer and for trace element concentrations by inductively coupled plasma-mass spectrometry (ICP-MS). For Cd, Pb, and As, the greatest contributions were found in the detoxified fractions, whereas the majority of total Hg was found in sensitive fractions. Selenium, an essential trace element, was distributed to a similar degree between the sensitive and detoxified compartments. Results indicate that although yelloweye sequestered and immobilized potentially toxic elements in detoxified fractions, the extent of binding differed among elements and followed the order: Cd > As > Pb > Hg. In yelloweye rockfish livers, the accumulation of non-essential elements at sensitive sites could lead to deleterious effects at the subcellular level, which should be evaluated in future studies. Article in Journal/Newspaper Prince of Wales Island Alaska Institut national de la recherche scientifique, Québec: Espace INRS Prince of Wales Island ENVELOPE(-99.001,-99.001,72.668,72.668) Environmental Pollution 242 63 72 |
institution |
Open Polar |
collection |
Institut national de la recherche scientifique, Québec: Espace INRS |
op_collection_id |
ftinrsquebec |
language |
English |
topic |
subcellular partitioning trace elements mercury detoxification yelloweye rockfish Alaska |
spellingShingle |
subcellular partitioning trace elements mercury detoxification yelloweye rockfish Alaska Barst, Benjamin D. Rosabal, Maikel Drevnick, Paul E. Campbell, Peter G. C. Basu, Niladri Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). |
topic_facet |
subcellular partitioning trace elements mercury detoxification yelloweye rockfish Alaska |
description |
Yelloweye rockfish (Sebastes ruberrimus) is an extremely long-lived species (up to ∼120 years) of fish, which inhabits the coastal waters of Alaska. Due to their long lifespans, yelloweye are known to accumulate high levels of mercury, and potentially other trace elements, in their tissues. Relatively little is known about the subcellular distribution of trace elements in the tissues of yelloweye rockfish; such information can provide important insights into detoxification/toxicity mechanisms at the subcellular level. To address this, we collected yelloweye rockfish (n = 8) from the eastern coast of Prince of Wales Island, Alaska in 2014. We determined the subcellular partitioning of trace elements (cadmium (Cd), lead (Pb), arsenic (As), total mercury (Hg), and selenium (Se)) in yelloweye livers with a partitioning procedure designed to separate liver cells into putative metal-sensitive fractions (cytosolic enzymes, organelles) and detoxified metal fractions (metallothionein or metallothionein-like proteins and peptides, granule-like structures) using differential centrifugation, NaOH digestion, and heat denaturation steps. The resulting fractions were then analyzed for total Hg with a direct Hg analyzer and for trace element concentrations by inductively coupled plasma-mass spectrometry (ICP-MS). For Cd, Pb, and As, the greatest contributions were found in the detoxified fractions, whereas the majority of total Hg was found in sensitive fractions. Selenium, an essential trace element, was distributed to a similar degree between the sensitive and detoxified compartments. Results indicate that although yelloweye sequestered and immobilized potentially toxic elements in detoxified fractions, the extent of binding differed among elements and followed the order: Cd > As > Pb > Hg. In yelloweye rockfish livers, the accumulation of non-essential elements at sensitive sites could lead to deleterious effects at the subcellular level, which should be evaluated in future studies. |
format |
Article in Journal/Newspaper |
author |
Barst, Benjamin D. Rosabal, Maikel Drevnick, Paul E. Campbell, Peter G. C. Basu, Niladri |
author_facet |
Barst, Benjamin D. Rosabal, Maikel Drevnick, Paul E. Campbell, Peter G. C. Basu, Niladri |
author_sort |
Barst, Benjamin D. |
title |
Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). |
title_short |
Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). |
title_full |
Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). |
title_fullStr |
Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). |
title_full_unstemmed |
Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). |
title_sort |
subcellular distributions of trace elements (cd, pb, as, hg, se) in the livers of alaskan yelloweye rockfish (sebastes ruberrimus). |
publishDate |
2018 |
url |
https://espace.inrs.ca/id/eprint/7653/ https://espace.inrs.ca/id/eprint/7653/1/P3364.pdf https://doi.org/10.1016/j.envpol.2018.06.077 |
long_lat |
ENVELOPE(-99.001,-99.001,72.668,72.668) |
geographic |
Prince of Wales Island |
geographic_facet |
Prince of Wales Island |
genre |
Prince of Wales Island Alaska |
genre_facet |
Prince of Wales Island Alaska |
op_relation |
https://espace.inrs.ca/id/eprint/7653/1/P3364.pdf Barst, Benjamin D., Rosabal, Maikel, Drevnick, Paul E., Campbell, Peter G. C. et Basu, Niladri (2018). Subcellular distributions of trace elements (Cd, Pb, As, Hg, Se) in the livers of Alaskan yelloweye rockfish (Sebastes ruberrimus). Environmental Pollution , vol. 242 . p. 63-72. DOI:10.1016/j.envpol.2018.06.077 <https://doi.org/10.1016/j.envpol.2018.06.077>. doi:10.1016/j.envpol.2018.06.077 |
op_rights |
cc_by_nc_nd_4 |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.envpol.2018.06.077 |
container_title |
Environmental Pollution |
container_volume |
242 |
container_start_page |
63 |
op_container_end_page |
72 |
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1766174419784302592 |