T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro
The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbo...
Published in: | Marine Pollution Bulletin |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2018
|
Subjects: | |
Online Access: | https://espace.inrs.ca/id/eprint/7462/ https://doi.org/10.1016/j.marpolbul.2017.12.001 |
id |
ftinrsquebec:oai:espace.inrs.ca:7462 |
---|---|
record_format |
openpolar |
spelling |
ftinrsquebec:oai:espace.inrs.ca:7462 2023-05-15T16:33:03+02:00 T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro Kleinert, Christine Lacaze, Emilie Fortier, Marlène Hammil, Mike De Guise, Sylvain Fournier, Michel 2018-02 https://espace.inrs.ca/id/eprint/7462/ https://doi.org/10.1016/j.marpolbul.2017.12.001 unknown Kleinert, Christine, Lacaze, Emilie, Fortier, Marlène, Hammil, Mike, De Guise, Sylvain et Fournier, Michel (2018). T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro Marine Pollution Bulletin , vol. 127 . p. 225-234. DOI:10.1016/j.marpolbul.2017.12.001 <https://doi.org/10.1016/j.marpolbul.2017.12.001>. doi:10.1016/j.marpolbul.2017.12.001 Immunotoxicity Marine mammal Pharmaceuticals Phoca vitulina Article Évalué par les pairs 2018 ftinrsquebec https://doi.org/10.1016/j.marpolbul.2017.12.001 2023-02-10T11:44:34Z The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals. Article in Journal/Newspaper harbor seal Phoca vitulina Institut national de la recherche scientifique, Québec: Espace INRS Marine Pollution Bulletin 127 225 234 |
institution |
Open Polar |
collection |
Institut national de la recherche scientifique, Québec: Espace INRS |
op_collection_id |
ftinrsquebec |
language |
unknown |
topic |
Immunotoxicity Marine mammal Pharmaceuticals Phoca vitulina |
spellingShingle |
Immunotoxicity Marine mammal Pharmaceuticals Phoca vitulina Kleinert, Christine Lacaze, Emilie Fortier, Marlène Hammil, Mike De Guise, Sylvain Fournier, Michel T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
topic_facet |
Immunotoxicity Marine mammal Pharmaceuticals Phoca vitulina |
description |
The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals. |
format |
Article in Journal/Newspaper |
author |
Kleinert, Christine Lacaze, Emilie Fortier, Marlène Hammil, Mike De Guise, Sylvain Fournier, Michel |
author_facet |
Kleinert, Christine Lacaze, Emilie Fortier, Marlène Hammil, Mike De Guise, Sylvain Fournier, Michel |
author_sort |
Kleinert, Christine |
title |
T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_short |
T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_full |
T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_fullStr |
T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_full_unstemmed |
T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro |
title_sort |
t lymphocyte-proliferative responses of harbor seal (phoca vitulina) peripheral blood mononuclear cells (pbmcs) exposed to pharmaceuticals in vitro |
publishDate |
2018 |
url |
https://espace.inrs.ca/id/eprint/7462/ https://doi.org/10.1016/j.marpolbul.2017.12.001 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_relation |
Kleinert, Christine, Lacaze, Emilie, Fortier, Marlène, Hammil, Mike, De Guise, Sylvain et Fournier, Michel (2018). T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro Marine Pollution Bulletin , vol. 127 . p. 225-234. DOI:10.1016/j.marpolbul.2017.12.001 <https://doi.org/10.1016/j.marpolbul.2017.12.001>. doi:10.1016/j.marpolbul.2017.12.001 |
op_doi |
https://doi.org/10.1016/j.marpolbul.2017.12.001 |
container_title |
Marine Pollution Bulletin |
container_volume |
127 |
container_start_page |
225 |
op_container_end_page |
234 |
_version_ |
1766022768769368064 |